# Partial Replacement of Soybean Protein (30%) with Nannochloropsis oceanica in Broiler Diets: Effects on Growth Performance and Meat Quality

**Authors:** Fabio Fanari, Joel Gonzalez, Anna Claret, Luis Guerrero, Borja Vilà, Massimo Castellari

PMC · DOI: 10.3390/foods15040760 · Foods · 2026-02-19

## TL;DR

Replacing 30% of soybean protein with microalgae in chicken feed slightly reduces growth but improves meat's n-3 fatty acids and carotenoids.

## Contribution

This study evaluates the effects of replacing soybean protein with Nannochloropsis oceanica in broiler diets.

## Key findings

- Microalgae diet slightly reduced animal growth due to lower feed digestibility.
- Meat from microalgae-fed chickens had higher n-3 fatty acids and carotenoids.
- No differences in shelf life or physicochemical parameters, except for meat color and exudate.

## Abstract

The use of human-edible materials like soy in animal feed raises several concerns, as it contributes to high greenhouse gas emissions and requires significant land and water use for agriculture. For this reason, research is exploring alternative ingredients rich in proteins like microalgae, which offer potential nutritional and environmental benefits. Species like Nannochloropsis are promising since their use for human consumption is very limited, making them non-competitive with human food. This article aims to formulate a poultry feed in which 30% of the crude protein from soybean meal is replaced by Nannochloropsis oceanica single-cell ingredients. Growth parameters have been evaluated in comparison with a diet based on soy protein. Additionally, the effect on meat quality was assessed by evaluating nutritional, texture, stability, and sensory parameters. Results showed that the microalgae diet caused a slight reduction in animal growth due to lower digestibility of the feed. Considering the quality parameters of the meat, no differences were found in terms of shelf life and physicochemical parameters, except for the color. The microalgae diet significantly increased the content of n-3 fatty acids and carotenoids in the meat. Finally, regarding sensory properties, the only change detected was in the amount of exudate.

## Linked entities

- **Chemicals:** carotenoids (PubChem CID 11227325)
- **Species:** Nannochloropsis oceanica (taxon 145522)

## Full-text entities

- **Diseases:** visual deterioration (MESH:C531604), food insecurity (MESH:D005517), Drip loss (MESH:C000726767), weight gain (MESH:D015430), atrophy (MESH:D001284), growth depression (MESH:D006130), injury to (MESH:D014947), muscle hypertrophy (MESH:C536106)
- **Chemicals:** Propyl gallate (MESH:D011435), biotin (MESH:D001710), essential amino acid (MESH:D000601), Cu (MESH:D003300), BHT (MESH:D002084), Fe (MESH:D007501), vitamin A (MESH:D014801), npP (MESH:C063701), EPA (MESH:D015118), water (MESH:D014867), ALA (MESH:D017962), Carotenoid (MESH:D002338), essential fatty acids (MESH:D005228), TAG (MESH:D014280), chlorophyll (MESH:D002734), tripentadecanoin (MESH:C000724032), Agar (MESH:D000362), polysaccharides (MESH:D011134), n-3 fatty acid (MESH:D015525), N2 (MESH:D009584), vitamin E (MESH:D014810), salt (MESH:D012492), Zn (MESH:D015032), O2 (MESH:D010100), calcium carbonate (MESH:D002119), methanol (MESH:D000432), vitamin K3 (MESH:D024483), NaCl (MESH:D012965), Mn (MESH:D008345), ether (MESH:D004986), FA (MESH:D005492), Ca (MESH:D002118), vitamin B6 (MESH:D025101), TBARS (MESH:D017392), DHA (MESH:D004281), chloroform (MESH:D002725), Lipid (MESH:D008055), vitamin B12 (MESH:D014805), I (MESH:D007455), calcium D-pantothenate (MESH:D010205), CO2 (MESH:D002245), citric acid (MESH:D019343), trichloroacetic acid (MESH:D014238), vitamin B2 (MESH:D012256), starch (MESH:D013213), MUFA (MESH:D005229), MDA (MESH:D008315), Fatty Acids (MESH:D005227), oils (MESH:D009821), acetone (MESH:D000096), Se (MESH:D012643), Minerals (MESH:D008903), nicotinic acid (MESH:D009525), vitamin B1 (MESH:D013831), vitamin D3 (MESH:D002762), amino acid (MESH:D000596), PUFA (MESH:D005231), hexane (MESH:D006586), sulfur amino acids (MESH:D000603), DM (-)
- **Species:** Spirulina (suborder) [taxon 551299], Sus scrofa (pig, species) [taxon 9823], Chlorella sp. (species) [taxon 3079], Enterovirus B (no rank) [taxon 138949], Escherichia coli (E. coli, species) [taxon 562], Glycine max (soybean, species) [taxon 3847], Malus domestica (apple, species) [taxon 3750], Nannochloropsis oceanica (species) [taxon 145522], Tetraselmis sp. (species) [taxon 2812566], Dunaliella (genus) [taxon 3044], Limnospira platensis (species) [taxon 118562], Nannochloropsis sp. (species) [taxon 52230], Gallus gallus (bantam, species) [taxon 9031], PX clade (clade) [taxon 569578], Enterobacteriaceae (enterobacteria, family) [taxon 543], Homo sapiens (human, species) [taxon 9606], Leptospira sp. AB (species) [taxon 103236]

## Full text

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## Figures

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## References

74 references — full list in the complete paper: https://tomesphere.com/paper/PMC12940817/full.md

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Source: https://tomesphere.com/paper/PMC12940817