# Anatomical and Transcriptomic Analyses Revealed the Key Genes Associated with Tuber Expansion in Cyperus esculentus L

## TL;DR

This study identifies key genes involved in tuber expansion in Cyperus esculentus, a tuber oil crop, using transcriptomic and anatomical analyses.

## Contribution

The study identifies 822 differentially expressed genes and 57 transcription factors linked to tuber expansion in Cyperus esculentus.

## Key findings

- Tuber expansion in Cyperus esculentus is primarily driven by cell expansion rather than cell number.
- Five genes encoding expansin proteins are significantly associated with cell wall loosening during tuber expansion.
- 822 differentially expressed genes and 57 transcription factors were identified as key regulators of tuber expansion.

## Abstract

Background: Cyperus esculentus L. is a unique tuber oil crop, in which tuber size directly determines both yield and oil storage capacity. It is crucial to clarify the tuber expansion pattern and explore the key genes associated with tuber expansion in Cyperus esculentus for crop improvement. Methods: This study conducted comprehensive morphological and cytological observations as well as transcriptomic analysis of tubers at multiple developmental stages. Tubers at 1, 5, 10, and 15 d were collected for transcriptome sequencing to identify differentially expressed genes (DEGs) and differentially expressed transcription factors. Gene ontology (GO) enrichment analysis was used to determine key functional categories. RT-qPCR was employed to verify the expression patterns of key genes. Results: Cyperus esculentus tubers expanded rapidly from 1 d to 15 d after initial tuber formation, and the expansion rate exhibited a trend of increasing first (1~5 d) and then decreasing (5~15 d). Cell expansion, rather than number, mainly contributed to tuber expansion. By combining the analysis of differential expression and the variation pattern of tuber expansion rate, 822 DEGs were identified to be associated with tuber expansion. GO enrichment analysis revealed that 20 genes were significantly enriched in GO:0043231 (cell wall), especially five remarkable genes encoding expansin, which exercise the function of cell wall loosening and have been proven to be associated with cell expansion. In addition, 57 differentially expressed TFs were further identified to be associated with tuber expansion. Conclusions: This study revealed the tuber expansion pattern of Cyperus esculentus and identified several key genes and TFs, which will facilitate the construction of the regulatory network and the analysis of the mechanism of tuber expansion in Cyperus esculentus.