# Human Microbiota-Associated Pig Models for Translational Microbiome Research: A Scoping Review

## TL;DR

This review explores how human microbiota-associated pig models can help bridge preclinical and translational research, focusing on methods and challenges.

## Contribution

The study provides a systematic evaluation and proposes a minimal reporting framework to standardize HMA pig model protocols.

## Key findings

- HMA pig models show promise for translational microbiome research but require standardized protocols.
- Variation in establishment methods affects microbiota engraftment outcomes in pigs.
- Factors influencing colonization efficiency and model reproducibility are identified.

## Abstract

The human microbiota-associated (HMA) pig model provides a physiologically relevant platform that bridges preclinical and translational research. However, its use remains limited, with existing studies showing considerable variation in establishment methods. This scoping review systematically evaluates methodological frameworks, engraftment outcomes, and research applications of HMA pig models. Additionally, it highlights their strengths, limitations, and implications for future studies. We conducted a comprehensive literature search in PubMed, Web of Science, Scopus, and Directory of Open Access Journals, following PRISMA guidelines for Scoping Reviews. The review examines the methodological foundations of HMA pig model generation and proposes a minimal reporting framework to promote standardization. It synthesizes studies on human microbiota engraftment in pigs, identifying factors that influence colonization efficiency. Finally, it summarizes current applications, discusses persistent limitations and translational challenges, and outlines opportunities for future research. Overall, these integrated insights aim to foster standardized, reproducible protocols for HMA pig model preparation and guide advancements in the field.