Targeting TRPA1 with Novel Synthetic Compounds Based on Different Scaffolds to Reduce Acute and Chronic Pain

TL;DR

Researchers developed new compounds targeting the TRPA1 receptor to potentially treat acute and chronic pain.

Contribution

A novel library of TRPA1-targeting synthetic compounds was designed and tested for analgesic effects.

Findings

Two compounds showed agonist activity and analgesic effects in a formalin-induced pain model.

Molecular docking helped explain the interaction of the compounds with TRPA1.

Modifications to the amide head and chain length influenced compound efficacy.

Abstract

The transient receptor potential ankyrin 1 (TRPA1) channel is a nonselective cation channel that detects noxious stimuli. Due to its role in acute and chronic pain transmission, interest in this receptor as a potential therapeutic target has grown. Among the natural compounds tested, δ-sanshool proved to be a promising modulator of TRPA1 due to its interaction with specific receptor cysteines. Starting from this polyunsaturated amide, we designed and prepared a small library of derivatives in which different amide heads were introduced and the length of the unsaturated chain was changed. The newly synthesized compounds were tested in vitro, and the results were rationalized by a molecular docking approach. Two of them, characterized by an agonist profile, were evaluated in vivo in the formalin-induced nociceptive response test, exhibiting promising analgesic properties.

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Full text

1. Introduction

Pain is a complex condition that the International Association for the Study of Pain defines as an unpleasant sensory and emotional experience associated with, or resembling that associated with, actual or potential tissue damage [1]. Undoubtedly, pain is a personal experience where biological, psychological, and social factors play a fundamental role [2]. Because of the complexity of pathophysiological mechanisms and heterogeneity of clinical conditions, pain is a significant unmet clinical need whose management is fundamental to avoid damage to biological systems and to allow the maintenance of a good quality of life [3].

Despite the availability of several analgesic and anti-inflammatory drugs, none of them can be considered sufficiently efficient and safe to play the role. Indeed, nonsteroidal anti-inflammatory drugs (NSAIDs) show varying but typically low levels of analgesic efficacy, while opioids, which represent the most effective class of analgesics, are associated with tolerance and dependence and induce behavioral addiction [2]. Advances in our understanding of the neurobiology of pain have contributed to define possible targets for the development of novel therapeutic strategies such as ion channels, enzymes and G-protein-coupled receptors (GPCRs) [4].

The family of transient receptor potential cation channels (TRPs) has progressively acquired interest over the years due to their key role in various intracellular pathways, such as protein expression regulation, membrane excitability, regulation of intracellular calcium levels and other homeostatic functions [5]. Among the seven different types of TRPs [6], the TRPA1 subfamily is considered a potential therapeutic target, since its expression in sensory neurons and epithelial cells has a crucial role in pathophysiological processes in numerous organ systems [7].

TRPA1 is a nonselective ionotropic channel whose structural organization is closely linked to its functional roles. Its N-terminal region contains an extended ankyrin repeat domain involved in protein–protein interactions, regulation of plasma membrane localization, channel trafficking, and an enhanced capacity for intracellular Ca^2+^ binding compared with other TRP channels [6]. Collectively, these structural and functional features underpin the role of TRPA1 as a sensor of cellular stress and tissue damage [8].

TRPA1 can be activated by mechanical, thermal, or chemical stimuli [9]. Chemical activation occurs through two distinct classes of agonists. Electrophilic agonists activate the channel by forming covalent adducts with critical cysteine residues (C621, C641, and C665) located in the pre-TM1 region, leading to conformational changes that promote channel opening. In contrast, non-electrophilic agonists activate TRPA1 and modulate channel gating without direct covalent interaction with the receptor [7]. Electrophilic agonists can be further classified into endogenous compounds, such as acrolein and reactive oxygen species, and exogenous compounds, including allyl isothiocyanate (AITC), cinnamaldehyde (CA), and allicin (Figure 1).

For its role in chronic inflammation and hyperalgesia, TRPA1 has been identified as a target for the development of analgesics, beyond a potential target in cancer and airway disease [10]. Several studies modulating TRPA1 activity have shown that this receptor is involved in inflammatory and immune responses [8,11]. In pain-associated pathological states, including diabetic neuropathy [12], osteoarthritis [13], and rheumatoid arthritis [14], endogenous agonists of TRPA1 are markedly increased. On the other hand, genetic ablation or pharmacological inhibition of TRPA1 has been shown to alleviate behavioral manifestations of allodynia and hyperalgesia in a variety of rodent pain models [8].

Several studies have shown that TRPA1 agonists not only activate the channel but also induce rapid functional desensitization, which can contribute to antinociceptive effects. For instance, newly reported non-electrophilic TRPA1 agonists have been shown to evoke TRPA1 activation followed by rapid current desensitization and significant analgesia in animal models, illustrating how agonist-driven desensitization can be exploited for pain modulation [15]. Agonist-induced TRPA1 desensitization has also been observed in sensory neurons with various chemical stimulants, supporting the dual role of TRPA1 ligands in initial activation and subsequent desensitization, which may underline peripheral antinociceptive mechanisms [16].

Among the numerous small-molecule TRPA1 antagonists reported since the discovery of the first prototypical TRPA1 antagonist, HC-030031, in 2007 [17], only ISC-17536 [18] and LY3526318 [19] have completed clinical proof-of-concept studies in patients with chronic pain conditions, highlighting the challenges associated with translating preclinical findings into clinical success. This complexity arises from multiple factors, including species-dependent differences in the physiological roles and tissue expression patterns of TRPA1, as well as the limitations of commonly used animal models, which primarily assess responses to acute noxious stimuli or evoked pain following nerve injury and may therefore fail to adequately reflect the ongoing spontaneous pain experienced by patients [20,21].

Unsaturated amides inspired by natural products have long been recognized as a relevant chemotype for TRPA1 modulation. In particular, Zanthoxylum-derived sanshools (e.g., hydroxy-α-sanshool) were among the first polyunsaturated amides shown to activate TRPA1 (and TRPV1) in sensory neurons, providing a key link between polyenic amide structures and TRPA1-mediated sensory responses [22,23]. Subsequent medicinal chemistry efforts expanded this concept through the synthesis of sanshool-derived alkylamides, demonstrating that the configuration and length of the conjugated double-bond system govern TRPA1 selectivity [24]. In parallel, N-cinnamoylanthranilates have been reported as human TRPA1 modulators and used to probe structure–activity relationships and binding-site determinants [25]. However, the direct use of long polyunsaturated chains poses practical limitations, including oxidative instability and formulation challenges, which can hinder systematic optimization [26].

Starting from this background and considering our interest in the identification of new TRPA1 modulators [27,28] and the experience gained in the chemistry of δ-sanshool [29], the objective of the present work was a rational, stability-oriented and systematically parameterized design of sanshool-inspired TRPA1 ligands, in which (i) the labile polyunsaturated tail is simplified and/or replaced with aromatic motifs to enhance chemical robustness, while retaining key unsaturation elements important for activity [25,30]; and (ii) distinct amide head groups (isobutyl, adamantyl, and hydroxyadamantyl) are combined with three complementary scaffold families to explicitly test steric, conformational, and polarity-driven hypotheses relevant to TRPA1 engagement.

On the basis of these design considerations, three complementary families of compounds were conceived to systematically investigate how variations in unsaturation pattern, linker architecture, and amide head group influence TRPA1 functional modulation (Figure 2):

Within each family, the carboxamide head group was varied among isobutyl, adamantyl, and hydroxyadamantyl substituents, while substitution patterns on the aromatic tail were systematically selected to systematically explore steric and electronic effects, including variations in substitution pattern, size, and electronic properties. In particular, meta-substituted aromatics were preferentially selected to mimic low-energy conformations of δ-sanshool [25], while additional substituents were introduced to probe hydrophobic and electronic interactions potentially relevant for TRPA1 modulation.

2. Results and Discussion

2.1. Chemistry

2.1.1. Family A

In a convergent synthetic approach, an appropriate aldehyde was reacted with a phosphonate to generate the trans diene system (Scheme 1).

The aldehyde 1, though commercially available, was synthesized by a Suzuki reaction between 4-bromobenzaldehyde and 4-pyridineboronic acid (Scheme 2), while the aldehydes 2–5 were the result of the alkylation of 3-hydroxybenzaldehyde with the appropriate halide derivative.

The phosphonates 6–8 (Scheme 3) were prepared starting from commercially available triethyl 4-phosphonocrotonate 9 [33]. The ester functionality was hydrolyzed to the corresponding acid 10 which was, in turn, converted to the amides 6–8 by reaction with the appropriate amine in the presence of a coupling agent. The Horner–Wadsworth–Emmons olefination reaction was conducted using the appropriate aldehydes in the presence of LiOH H_2_O and 4 Å molecular sieves to provide preferentially the trans isomers 11–26 in good-to-excellent yields [34].

2.1.2. Family B

Compounds of this family were prepared using different synthetic approaches. Compounds 27 and 28 were obtained by amidation reaction between trans-cinnamic acid and 1-aminoadamantane or 3-amine-1-adamantanol, respectively (Scheme 4), using EDC and HOBt as coupling agents.

The preparation of compound 29 started from 4-bromocinnamic acid (Scheme 5), which was converted to the corresponding amide 30 by reaction with 1-aminoadamantane. Suzuki coupling in the presence of 4-pyridineboronic acid eventually afforded compound 29.

Compounds 31–34 were prepared according to Scheme 6. The Horner–Wadsworth–Emmons olefination reaction between triethyl phosphonoacetate and the appropriate aldehyde led to intermediates 35 and 36 which were hydrolyzed to the corresponding acids 37 and 38. Reaction with 1-aminoadamantane or 3-amine-1-adamantanol afforded final compounds 31 and 33 or 32 and 34, respectively.

2.1.3. Family C

The triazole compound 39 (Scheme 7) was prepared by a coupling reaction between 1-aminoadamantane and 1-(4-bromophenyl)-1H-1,2,3-triazole-4-carboxylic acid (40) which was synthesized according to a literature procedure [35].

Although no systematic stability studies were performed, selected compounds stored at room temperature in the dark for extended periods did not show detectable changes in their ^1^H-NMR spectra, indicating the absence of major degradation products under these storage conditions.

2.2. In Vitro Studies

2.2.1. In Vitro Functional Studies at TRPA1

The activity of the tested compounds is summarized in Table 1, which reports both their efficacies and potencies in eliciting TRPA1-mediated increases in Ca^2+^ influx, as well as their desensitizing effects against allyl isothiocyanate (AITC) at this receptor channel [36,37,38]. The active compounds show an IC_50_ in the low micromolar range and, besides 29 which is a pure antagonist, all behave as partial agonists/desensitizing agents. Compounds 18 and 39 were not tested due to solubility issues.

Within Family A, a clear SAR emerges when comparing the nature of the amide head group. Across multiple matched pairs (14–15, 16–17, 22–23, 19–20, 25–26), replacement of the isobutyl moiety with an adamantyl group results in a marked gain in TRPA1 activity. While isobutyl derivatives are largely inactive (with the exception of 19 and 21), the corresponding adamantyl analogues display partial agonism with EC_50_ values ranging from 0.86 to 4.1 µM and desensitization IC_50_ values in the low micromolar range (3.7–6.9 µM). The most pronounced improvement is observed for compound 17, which shows submicromolar potency (EC_50_ = 0.86 µM) compared with its isobutyl counterpart 16, which is inactive under the same conditions, indicating a >10-fold gain in functional potency associated with increased steric bulk at the amide position.

Among the active adamantyl derivatives, the size and topology of the aromatic tail further modulate potency. Simple phenyl substitution (compound 12) results in moderate activity (EC_50_ = 11.5 µM), whereas extension to bulkier biaryl systems leads to a consistent increase in potency. The naphthyl and biphenyl-containing derivatives 14 and 17 display EC_50_ values of 3.1 and 0.86 µM, respectively, corresponding to an approximately 4–13-fold improvement relative to compound 12. These data indicate that increased aromatic surface and rigidity enhance productive interactions with TRPA1. Among these compounds, 17 turned out to be the most promising dual modulator, with submicromolar potency as an agonist and strong desensitizing ability (IC_50_ = 3.7 μM). Similar behavior is shown by the less potent allyloxyphenyl derivative 26 (EC_50_ = 4.1 μM, IC_50_ = 6.9 μM). Compound 19 (phenoxyphenyl, isobutyl) exhibits measurable agonist activity (EC_50_ = 0.45 µM) and strong desensitization (IC_50_ = 2.5 µM), whereas its adamantyl analogue 20 is inactive, highlighting an inverse SAR trend between the two amide series.

Within Family B compounds, characterized by the presence of a single double bond, most derivatives are inactive, indicating that reduced conjugation and/or the shortening of the linker disfavors productive interactions with the receptor. Notable exceptions include compounds 30 and 32, which retain partial agonist/desensitizing profiles with EC_50_ values of 1.21 and 26.5 µM, respectively. In these cases, activity is restored by the presence of either a strongly electron-withdrawing substituent (Br in 30) or an additional hydrogen-bond donor (hydroxyadamantyl in 32), compensating for the shorter linker. Conversely, compound 29 behaves as a pure antagonist (IC_50_ = 4.8 µM), indicating that introduction of a heteroaromatic ring shifts the functional profile rather than simply reducing potency.

2.2.2. Competitive Binding Assay at CB Receptors

The results of this assay indicate that the tested compounds show negligible affinity for cannabinoid receptors. In the few instances where IC_50_ values could be determined (i.e., below 10 µM), the activity remains in the low micromolar range, suggesting weak potency and limited pharmacological relevance at CB receptors.

2.3. Homology Modelling of Rat TRPA1

To better align the docking studies with the biological data performed on rat TRPA1 (rTRPA1), we used as target protein homology models of rTRPA1 in both open and closed states, built onto the cryo-EM structures of the human ortholog (hTRPA1). In particular, compounds with an agonist profile were docked onto an rTRPA1 3D model in the activated form [6], built using the hTRPA1 cryo-EM structure in complex with an electrophilic agonist (PDB id:6PQO) [39], while for the pure antagonist 29 two rTRPA1 homology models were built using as templates the hTRPA1 cryo-EM structures in complex with antagonists bound at two different sites: one at the interface of two monomers close to the pore loop (Site1ant, PDB id:6WJ5 [40]) and the other close to the TRP helix of each monomer (Site2ant, PDBid:7OR0). As for the antagonists, two binding sites have been identified for non-covalent agonists: Site1ago, located between the VSLD domain of one monomer and the transmembrane helices S5–S6 of the adjacent monomer, unveiled by the hTRPA1 cryo-EM structure in complex with a non-covalent agonist (PDB id:6X2J, [41]); and Site2ago, located in a pocket formed by the pore helix, S5–S6 transmembrane helices of each monomer and the S6 transmembrane helix of the adjacent monomer, in the same region of Site1ant and previously identified by mutagenesis [42]. Since only the experimental complex of a hTRPA1 agonist at Site1ago is available, to avoid bias, we used as a template for the homology model of rTRPA1 in the activated form a cryo-EM structure of hTRPA1 in complex with an electrophilic agonist, whose binding site in located in the ankyrin domain, far from the binding sites for the non-covalent agonists. In fact, in the cryo-EM structure used as a template (PDB id: 6PQO), Site1ago and Site2ago are both occupied by lipid molecules. Although the sequence identity between rat and human TRPA1 is high (~80%), not all the residues in the binding sites are conserved, as shown in Supplementary Figure S1. At Site1ago, there are four non-conserved residues between human and rat (rTRPA1 numbering): Ile806Tyr, Tyr843Phe, Leu870Phe and Ser936Ala, respectively. These contribute to increase both the aromatic and the hydrophobic nature of rTRPA1 Site1ago respect to the human ortholog. At Site2ago/Site1ant, two residues are not conserved: Ile908Leu and Ile939Met.

2.4. Molecular Docking Studies

Looking at the biological activity of the agonist compounds, an opposite trend has been observed between the isobutyl and adamantyl derivatives: the substituents beneficial for the activity in the isobutyl series are detrimental for the adamantyl one and vice versa. To evaluate if this trend arises from a preferential binding of the two families into different sites, we performed a docking run on compounds 17 and 19, representative of the adamantyl and isobutyl series, respectively, using a grid box encompassing both sites. Indeed, compound 17 was found to only bind Site1ago in two opposite orientations, with the amide bond facing Tyr806 (−9.04 kcal/mol) or Gln943 (−8.97 kcal/mol) (Figure 3C,D). The dual orientation at Site1ago is promoted by the occurrence of Tyr806, which can be involved in a H-bond with the ligand amide group, differently from the human ortholog where the same position is occupied by an isoleucine. Instead, compound 19 was found to bind Site2ago with a more favorable binding energy (−7.62 kcal/mol) in comparison to Site1ago (−6.84 kcal/mol) (Figure 4). At Site2ago, the isobutyl group forms hydrophobic interactions mainly involving leucine and valine residues at the interface between monomers A and B, whereas at monomer A it engages in aromatic interactions with the phenylalanine residues Phe887, Phe912 and Phe947 and in a H-bond with Thr877.

The larger and aromatic Site1ago also allows the accommodation of bulkier pendant groups, as in the case of compounds 14 and 17, which are not active in the correspondent isobutyl series. On the other hand, the narrow Site2ago only allows the accommodation of flexible pendant groups, as in the case of compounds 19 and 21. To elucidate the binding mode of active compounds featuring the shorter linker, docking studies were also performed on compounds 30 and 32 at Site1ago. As for 17, compound 30 can adopt two reversed poses (Figure 3A,B), with the amide group alternatively pointing toward the polar residues Gln943 (monomer A, pose 1, −8.00 kcal/mol) or Tyr806 (monomer B, pose 2, −8.33 kcal/mol).

In both orientations, the phenyl moiety of compound 30 is engaged in aromatic π-π stacking with the aromatic residues of Site1ago, being sandwiched between Phe950 (monomer A) and Phe870 (monomer B), in pose 1, and being hosted in an aromatic cleft formed by Phe843, Phe844 (monomer B) and Phe887 (monomer A) in pose 2. In this latter pose, the bromine atom is involved in polar interactions with the Gln943 sidechain. The bromine atom is critical for the activity, since the corresponding derivative with the unsubstituted phenyl ring is inactive (compound 30 vs. compound 27). Thus, the occurrence of the bromine atom induces an extra-stabilization due to polar interactions with either Gln943 or Tyr806 residues which compensates for the shorter linker chain. In compound 32, the hydroxy group on the adamantyl moiety is critical for the activity, since the unsubstituted derivative 31 is inactive. Docking of compound 32 at Site1ago shows that the hydroxy group is close to Ser890 and Gln943, forming a H-bond with the latter (−8.75 kcal/mol, pose1), and, in the reverse orientation, with the Glu867 sidechain (−9.41 kcal/mol, pose2) on the other monomer (see Figure 5).

Finally, the replacement of the terminal phenyl group with a pyridine moiety in the compound 31 scaffold promotes a shift toward pure antagonism, as observed for compound 29. To rationalize the role of the pyridine moiety, this compound was docked at both antagonist binding sites, i.e., Site1ant and Site2ant. As shown in Figure 6, at Site1ant the pyridine ring forms a stabilizing H-bond with Ser876 (−9.78 kcal/mol, Figure 6A), orienting the ligand in a binding pose orthogonal to what is observed for the agonist 30, while at Site2ant it is involved in a H-bond with the Arg706 sidechain (−9.65 kcal/mol, Figure 6B), corresponding to a lysine residue in the hTRPA1 sequence (see Supplementary Figure S1). At this site, an alternative binding pose was found, with the amide bond involved in a H-bond interaction with Asn858 and with the pyridine ring surrounded by polar residues (−7.98 kcal/mol, Supplementary Figure S2). In both cases, the nitrogen atom of the pyridine ring plays a key role in stabilizing the binding at the antagonist sites, thus explaining the switch toward the pure antagonism of compound 29.

2.5. In Vivo Studies

Based on the in vitro findings, compounds 30 and 17 were selected for in vivo studies. Their analgesic properties were evaluated using the formalin test, a widely used model for the evaluation of the efficacy of analgesic and anti-inflammatory agents [43], while their ability to reduce neuropathic pain was evaluated in the spared nerve injury (SNI) mouse model (Figure 7) [31].

A single intra-paw injection of 30 (1, 2 and 3 μg/mouse, 20 μL) prevented pain behavior in the second phase of the formalin test in a dose-dependent manner, as compared to the vehicle [time 35 min for 30 (3 μg/mouse): 0.42 s ± 0.145 vs. 1.39 s ± 0.15; F_(3,18)_ = 8.465; p = 0.0010]. Pretreatment with AP18 (0.05 mg/kg, i.p.) counteracted the antinociceptive effects of 30 during the delayed time of the late phase (from 15 min onwards) and it increased noxious behaviors during the first 10 min of the second phase.

A single intra-paw injection of 17 (1, 2 and 3 μg/mouse, 20 μL) prevented pain behavior in both phases of the formalin test in a dose-dependent manner, as compared to the vehicle [time 5 min for 17 (3 μg/mouse): 2.70 s ± 0.52 vs. 4.55 s ± 0. 47; time 35 min for 17 (3 μg/mouse): 0.48 s ± 0.21 vs. 2.40 s ± 0.6; F_(2,13)_ = 12.37; p = 0.0010]. Treatment with AP18 (0.05 mg/kg, i.p.) prevented the antinociceptive effect of 17.

Finally, for compound 17 only, the antiallodynic effect was also tested by systemic injection (intraperitoneal, i.p.). A single administration of compound 17 (0.5, 1, and 2 mg/kg, i.p.) inhibited allodynia in neuropathic mice with spared nerve injury (SNI) of the sciatic nerve: the antiallodynic effect induced by 17 was dose-dependent, compared to the vehicle [time 60 min for 17 (1 mg/kg): 0.4880 g ± 0.157 vs. 0.027g ± 0.007; time 40 and 60 min for 17 (2 mg/kg): 0.574 g ± 0.148 vs. 0.02 0g ± 0.00 and 0.734 ± 0.183 vs. 0.027 ± 0.007. F_(3,17)_ = 9.17; p ≤ 0.0001].

The two molecules 30 and 17 show a slightly different pharmacodynamic profile in vivo: 17 appears to recapitulate the characteristics observed with some potent TRPA1 receptor agonists, which may subsequently induce receptor desensitization upon increasing doses or prolonged stimulation, thereby effectively behaving as a “functional” receptor antagonist at higher doses.

In our experiments, the behavior of 17 as a potent agonist and desensitizer of TRPA1 is suggested by the following facts: (a) at the minimum dose of 1 µg/mouse, it potentiates the effect of formalin which is itself an activator of the TRPA1 receptor [17]; (b) unlike 30, it prevents both the first and the second delayed nocifensive phase of formalin. Our in vivo data clearly show a dose-dependent biphasic action of 17 on TRPA1 channels, with an initial activation followed by a more intense and persistent desensitization and subsequent pain reduction. The role of TRPA1 receptors in the action of 17 is further confirmed in vivo by AP18, which prevents its analgesic effects. AP-18 is a reference TRPA1 antagonist that we have already evaluated in the formalin assay and also in other assays.Therefore, based on our experience in in vivo experiments, we also used it in this current study.

Compound 30, instead, seems to behave in vivo as a TRPA1 antagonist up to inhibiting the effect of formalin and this activity is occluded by another selective TRPA1 antagonist, AP18. Interestingly, AP18 pretreatment, while counteracting the antinociceptive effects of 30 during the late phase (from 15 min onwards), briefly increased pain-related behaviors during the first 10 min after the onset of the second formalin phase. One explanation for this paradoxical effect is the possible off-target action of 30 that would be unmasked by blocking TRPA1 with AP18. In fact, it should not be underestimated that, in addition to TRPA1, in the second phase of the formalin test there are multiple receptors and neurotransmitter systems that determine the different components of this late and prolonged painful phase [44].

Indeed, it is worth noting that AP-18-induced TRPA1 receptor blockade could potentially generate paradoxical effects due to off-target effects. However, although an off-target contribution cannot be entirely excluded, the very low dose used, the selective reduction in aldehyde-induced pain, and the complete absence of other behavioral changes support the conclusion that the observed effects are predominantly on-target, even in the presence of AP-18.

Beyond these considerations, our in vivo data, albeit preliminary, suggest that the investigated compounds exert pharmacological activity in vivo involving TRPA1 receptors, which are obligatorily required for generating formalin-induced pain.

Indeed, there is evidence that both the pharmacological blockade and the genetic deletion of TRPA1 prevent the nocifensive responses of startle, licking and lifting from intra-paw injection of formalin, confirming the aldehyde as a pharmacophore necessary for the activation of these nociceptors. Furthermore, in our data on mice with peripheral neuropathy, 17 shows therapeutic efficacy through a reduction in tactile allodynia. These experiments confirm the versatility of these molecules in the treatment of different chronic pain syndromes, both inflammatory and neuropathic. Obviously, further studies will be needed to define in more detail the actual potential of these compounds or their structural analogues.

Finally, it is important to note that the distinctive characteristics of the adamantane scaffold are extremely valuable for specific biological applications. Its intrinsic lipophilicity and ability to improve drug stability, can lead to improved pharmacokinetic properties of drug candidates. Moreover, the rigid cage-like structure protects the pharmacophoric groups from metabolic degradation, thus preserving molecular stability and diffusion in blood plasma and, consequently, the possibility of its intact arrival in “sanctuary” regions such as the CNS. Further studies will aim to further explore these aspects and also to understand whether they exhibit unique kinetics, with a more direct tropism toward the spinal cord or even CNS structures involved in pain control.

3. Materials and Methods

3.1. Chemistry

Commercially available reagents from Merck (Merck KGaA, Darmstadt, Germany) were used as received unless otherwise specified. Solvents were treated before use with suitable drying agents and used after distillation in an atmosphere of N_2_. Reactions requiring anhydrous conditions were performed under N_2_. Organic solutions were dried over anhydrous Na_2_SO_4_. Evaporation was carried out in vacuo using a rotating evaporator. Melting points were determined on a Gallenkamp apparatus (Gallenkamp, London, United Kingdom) and are uncorrected. For flash chromatography, Merck Kieselgel 60 (0.040–0.063 mm) was used. Thin-layer chromatography (TLC) was performed on silica gel plates (Merck 60 F254) vailable from Merck (Merck KGaA, Darmstadt, Germany) eluting with the solvents indicated, visualized by a 254 nm UV lamp (λ = 254 nm) and stained with aqueous potassium permanganate, Pancaldi and phosphomolybdic acid solutions. ^1^H NMR and ^13^C NMR spectra were recorded on a Brucker 400 Advance, Brucker 600 Advance (Bruker Italia srl, Milan, Italy) or Agilent VNMRS500 spectrometer (Agilent Technologies Italia, Cernusco sul Naviglio, Milan, Italy). Chemical shifts (δ) and coupling constants (J) are reported in parts per million (ppm) and hertz (Hz), respectively. Mass spectra were recorded with an LC-MSD 1100 series Agilent instrument (Agilent Technologies Italia, Cernusco sul Naviglio, Milan, Italy), with electrospray interface and with a 0.4 mL/min flow rate using a binary solvent system of 95:5 methanol/water. The UV detector was set at 254 nm and the mass spectra were acquired either in positive or in negative mode scanning over the mass range of 105–1500. High-resolution mass spectrometry analyses have been performed using a Bruker TimsTOF instrument (Bruker Italia srl, Milan, Italy) equipped with an ESI source, in positive or negative mode, dry gas 6.0 L/m, dry temperature 210 °C, nebulizer at 3.0 bar, and capillary at 4500 V.

3.1.1. General Procedure for the Synthesis of Compounds 1 and 29 by Suzuki Coupling

4-Bromobenzaldehyde or compound 30 (0.08 mmol), 4-pyridineboronic acid (0.16 g, 1.80 mmol), K_2_CO_3_ (0.75 g, 5.41 mmol) and Pd(PPh_3_)4 (0.06 g, 0.05 mmol) were added to 1,4-dioxane:H_2_O (10:1, 10 mL). The reaction mixture was refluxed overnight under a N_2_ atmosphere. After cooling, the reaction mixture was filtered through a Celite^®^ pad and evaporated. The residue was solubilized in EtOAc, washed with brine, dried over anhydrous Na_2_SO_4_ and evaporated in vacuo. The residue was purified by flash chromatography on silica gel.

4-(Pyridin-4-yl)benzaldehyde (1)

Eluent: PE/EtOAc 7:1. Yellow solid (97% yield). Mp 87–90 °C (lit. [45] 87–88 °C). ^1^H NMR (400 MHz, CDCl_3_): δ 10.03 (s, 1H), 8.68 (d, J = 5.0 Hz, 2H), 7.95 (d, J = 7.8 Hz, 2H), 7.75 (d, J = 7.9 Hz, 2H), 7.54 (d, J = 5.0 Hz, 2H).

(E)-N-(Adamantan-1-yl)-3-(4-(pyridin-4-yl)phenyl)acrylamide (29)

Eluent: PE/Et_2_O 1:4 and then Et_2_O/EtOAc 9:1. White solid (54% yield). Mp 227–229 °C. ^1^H NMR (400 MHz, CD_3_OD): δ 8.52 (d, J = 5.5 Hz, 2H), 7.87–7.55 (m, 6H), 7.41 (d, J = 15.7 Hz, 1H), 6.63 (d, J = 15.7 Hz, 1H), 2.04 (s, 10H), 1.69 (s, 5H). ^13^C NMR (100 MHz, DMSO-d6): δ 164.4, 150.8, 146.7, 138.0, 137.4, 136.6, 128.6, 127.8, 125.2, 121.5, 51.4, 41.5, 36.5, 29.3. MS (ESI): m/z 359.1 [M + H]^+^ (70), 381.1 [M + Na]^+^ (100), 739.3 [2M + Na]^+^ (90).

3.1.2. General Procedure for the Preparation of Aldehydes 2–5

A solution of 3-hydroxybenzaldehyde (0.20 g, 1.64 mmol) in dry DMF (10 mL) was treated with potassium carbonate (0.27 g, 1.97 mmol). The resulting mixture was heated to 50 °C and then slowly treated with the appropriate alkyl halide (1.97 mmol). The reaction mixture was stirred at 50 °C for 3 h. After cooling, the reaction mixture was diluted with H_2_O and extracted with Et_2_O. The organic solution was washed with 5% NaOH and brine, dried over anhydrous Na_2_SO_4_, filtered and evaporated in vacuo to give the title compounds which were used as such in the next step.

3-(Pentyloxy)benzaldehyde (2) [46]. Colorless oil (0.26 g, 84% yield). MS (ESI): m/z 193 [M + H]^+^ (90). ^1^H NMR (600 MHz, CDCl_3_) δ 9.98 (s, 1H), 7.46–7.44 (m, 2H), 7.41–7.39 (m, 1H), 7.21–7.15 (m, 1H), 4.03 (t, J = 6.6 Hz, 2H), 1.83 (dq, J = 13.3, 6.6 Hz, 2H), 1.51–1.37 (m, 4H), 0.96 (t, J = 7.2 Hz, 3H). ^13^C NMR (151 MHz, CDCl_3_) δ 192.2, 159.7, 137.8, 130.0, 123.3, 121.9, 112.8, 68.3, 28.8, 28.2, 22.4, 14.0. HRMS ESI m/z: calc. for C_12_H_17_O_2_^+^ = 193.12231, found = 193.12232 [M + H]^+^; calc. for C_12_H_16_NaO_2_^+^ = 215.10425, found = 215.10432 [M + Na]^+^; calc. for C_13_H_20_NaO_3_^+^ = 247.13047, found = 247.13045 [M + Na + MeOH]^+^.3-((Cinnamyloxy)benzaldehyde (3) [47]. Colorless oil (0.10 g, 65% yield). MS (ESI): m/z 238 [M + H]^+^ (100). ^1^H NMR (600 MHz, CDCl_3_) δ 10.01 (s, 1H), 7.52–7.47 (m, 3H), 7.45 (d, J = 7.7 Hz, 2H), 7.36 (t, J = 7.5 Hz, 2H), 7.32–7.26 (m, 2H), 6.79 (d, J = 16.0 Hz, 1H), 6.44 (dt, J = 15.9, 5.8 Hz, 1H), 4.79 (d, J = 5.8 Hz, 2H). ^13^C NMR (151 MHz, CDCl_3_) δ 192.1, 159.2, 137.9, 136.3, 133.6, 130.2, 128.7, 128.1, 126.6, 123.7 (2C), 122.2, 113.3, 68.9. HRMS ESI m/z: calc. for C_16_H_14_NaO_2_^+^ = 261.08860, found = 261.08855 [M + Na]^+^.3-(Allyloxy)benzaldehyde (4) [48]. Yellow oil (0.28 g, 86% yield). MS (ESI): m/z 185 [M + Na]^+^ (100). ^1^H NMR (600 MHz, CDCl_3_) δ 9.96 (s, 1H), 7.47–7.41 (m, 2H), 7.39 (dd, J = 2.6, 1.2 Hz, 1H), 7.19 (ddd, J = 7.6, 2.6, 1.7 Hz, 1H), 6.05 (ddt, J = 17.2, 10.5, 5.2 Hz, 1H), 5.43 (dq, J = 17.3, 1.6 Hz, 1H), 5.31 (dq, J = 10.5, 1.4 Hz, 1H), 4.59 (dt, J = 5.3, 1.5 Hz, 2H). ^13^C NMR (151 MHz, CDCl_3_) δ 192.1, 159.1, 137.8, 132.7, 130.1, 123.5, 122.0, 118.0, 113.2, 68.9. HRMS ESI m/z: calc. for C_10_H_10_NaO_2_^+^ = 185.05730, found = 185.05730 [M + Na]^+^; calc. for C_11_H_14_NaO_3_^+^ = 217.08352, found = 217.08361 [M + Na + MeOH]^+^.3-((3-Methylbut-2-en-1-yl)oxy)benzaldehyde (5) [49]. Yellow oil (0.28 g, 89% yield). MS (ESI): m/z 213 [M + Na]^+^ (100), 191 [M + H]^+^ (40). ^1^H NMR (600 MHz, CDCl_3_) δ 10.00 (s, 1H), 7.49–7.44 (m, 2H), 7.44–7.42 (m, 1H), 7.21 (d, J = 7.0 Hz, 1H), 5.52 (t, J = 6.7 Hz, 1H), 4.60 (d, J = 6.7 Hz, 2H), 1.83 (s, 3H), 1.79 (s, 3H). ^13^C NMR (151 MHz, CDCl_3_) δ 192.2, 159. 5, 138.9, 137.8, 130.0, 123. 5, 122.3, 119.1, 112.9, 65.1, 25.8, 18.3. HRMS ESI m/z: calc. for C_12_H_14_NaO_2_^+^ = 213.08860, found = 213.08859 [M + Na]^+^; calc. for C_13_H_18_NaO_3_^+^ = 245.11482, found = 245.11481 [M + Na + MeOH]^+^.

3.1.3. (E)-4-(Diethoxyphosphoryl)but-2-enoic Acid (10) [50]

A solution of KOH (3.69 g, 65.82 mmol) in H_2_O (90 mL) was added to a solution of 9 (12.66 g, 50.63 mmol) in H_2_O (90 mL) and the reaction mixture was stirred at r.t. for 6 h. Subsequently, the crude product was extracted with Et_2_O to remove the starting material. The aqueous solution was treated with conc. HCl until pH 1 and then it was extracted (3x) with EtOAc. The organic solution was washed with brine, dried over Na_2_SO_4_ and evaporated to dryness to give a white solid (7.266 g, 65% yield). Mp 73–75 °C. ^1^H NMR (600 MHz, DMSO-d6) δ 12.38 (br s, 1H), 6.65 (dq, J = 15.3, 7.8 Hz, 1H), 5.95 (ddt, J = 15.5, 4.9, 1.4 Hz, 1H), 4.05–3.98 (m, 4H), 2.89 (ddd, J = 22.7, 7.8, 1.4 Hz, 2H), 1.23 (t, J = 7.1 Hz, 6H). ^13^C NMR (151 MHz, DMSO-d6) δ 167.0 (d, ^4^J_C-P_ = 2.7 Hz), 138.7 (d, ^3^J_C-P_ = 10.9 Hz), 126.3 (d, ^2^J_C-P_ = 13.6 Hz), 62.0 (d, ^2^J_C-P_ = 6.4 Hz), 29.6 (d, ^1^J_C-P_ = 134.7 Hz), 16.7 (d, ^3^J_C-P_ = 5.8 Hz). HRMS ESI m/z: calc. for C_8_H_15_NaO_5_P^+^ = 245.05493, found = 245.05514 [M + Na]^+^; calc. for C_16_H_30_NaO_10_P_2_^+^ = 467.12064, found = 467.12189 [2M + Na]^+^.

3.1.4. General Amidation Procedure for the Synthesis of Phosphonates 6–8 and Final Compound 39

HOBt (0.61 g, 4.50 mmol), HBTU (3.41 g, 9.00 mmol), DIPEA (0.87 g, 6.75 mmol) and the appropriate amine (4.5 mmol) were added to a solution of 10 or 40 (4.5 mmol) in THF:CH_3_CN 1:1 (75 mL). The reaction was stirred under a N_2_ atmosphere at r.t. for 18–23 h, then the solution was evaporated to dryness in vacuo. Subsequently, the crude product was dissolved in EtOAc and the solution was washed with H_2_O, saturated solution of Na_2_CO_3_ and brine. The organic phase was then dried over anhydrous Na_2_SO_4_, filtered, and evaporated to give a residue.

Diethyl ((E)-4-((Adamantan-1-yl)amino)-4-oxobut-2-en-1-yl)phosphonate (6)

Prepared from 10 and 1-aminoadamantane. Reaction time: 18 h. The yellow oil was purified by flash chromatography (eluent, CH_2_Cl_2_:CH_3_OH 98:2) to give 6 as a white solid (74% yield). Mp 115–118 °C. ^1^H NMR (600 MHz, CD_3_OD) δ 6.60 (dq, J = 15.2, 7.6 Hz, 1H), 6.10 (dd, J = 15.2, 5.1 Hz, 1H), 4.18–4.10 (m, 4H), 2.83 (dd, J = 22.8, 7.8 Hz, 2H), 2.11–2.05 (m, 9H), 1.78–1.72 (m, 6H), 1.35 (t, J = 7.1 Hz, 6H). ^13^C NMR (151 MHz, CD_3_OD) δ 165.1 (d, ^4^J_C-P_ = 2.7 Hz), 131.1 (d, ^3^J_C-P_ = 11.4 Hz), 129.5 (d, ^2^J_C-P_ = 13.9 Hz), 62.4 (d, ^2^J_C-P_ = 6.8 Hz), 51.6, 40.9, 36.1, 29.5, 28.8 (d, ^1^J_C-P_ = 138.6 Hz), 15.3 (d, ^3^J_C-P_ = 6.0 Hz). HRMS ESI m/z: calc. for C_18_H_30_NNaO_4_P^+^ = 378.18047, found = 378.18019 [M + Na]^+^.

Diethyl ((E)-4-((3-Hydroxyadamantan-1-yl)amino)-4-oxobut-2-en-1-yl)phosphonate (7)

Prepared from 10 and 1-hydroxyadamantane-3-amine. Reaction time: 24 h. The yellow oil was purified by flash chromatography (eluent, CH_2_Cl_2_:CH_3_OH 96:4) to give 7 as a white solid (78% yield). ^1^H NMR (600 MHz, CD_3_OD) δ 6.61 (dq, J = 15.2, 7.6 Hz, 1H), 6.11 (dd, J = 15.2, 4.2 Hz, 1H), 4.18–4.10 (m, 4H), 3.23 (q, J = 7.3 Hz, 2H), 2.83 (dd, J = 22.8, 7.8 Hz, 2H), 2.25 (br s, 2H), 1.98–1.96 (m, 4H), 1.70 (q, J = 11.6 Hz, 4H), 1.60 (dd, J = 30.4, 12.5 Hz, 2H), 1.37–1.31 (m, 6H). ^13^C NMR (151 MHz, CD_3_OD) δ 165.2 (d, ^4^JC-P = 2.5 Hz), 131.3 (d, ^3^JC-P = 11.4 Hz), 129.4 (d, ^2^JC-P = 14.1 Hz), 68.2, 62.5 (d, ^2^JC-P = 6.7 Hz), 54.0, 48.0, 46.6, 43.5, 39.7, 34.7, 30.7, 28.8 (d, ^1^JC-P = 138.6 Hz), 15.3 (d, ^3^JC-P = 5.9 Hz). HRMS ESI m/z: calc. for C_18_H_31_NO_5_P^+^ = 372.19344, found = 372.19336 [M + H]^+^; calc for. C_18_H_30_NNaO_5_P^+^ = 394.17538, found = 394.17534 [M + Na]^+^; calc. for C_18_H_30_KNO_5_P^+^ = 410.14932, found = 410.14946 [M + K]^+^.

3.1.5. Diethyl (E)-(4-(Isobutylamino)-4-oxobut-2-en-1-yl)phosphonate (8) [30]

EDC (5.75 g, 0.03 mol) and HOBt (2.43 g, 0.018 mol) were added to a solution of 10 (3.33 g, 0.015 mol) in dry CH_2_Cl_2_ (60 mL). Subsequently, a solution of isobutyl amine (2.20 g, 0.03 mol) in dry CH_2_Cl_2_ (20 mL) was added and the reaction mixture was stirred at r.t. for 3 h. After, a saturated solution of Na_2_CO_3_ was added and the aqueous phase was extracted with CH_2_Cl_2_ (3x). The organic solution was washed with brine, dried over anhydrous Na_2_SO_4_, filtered and evaporated to give a yellow oil. The crude product was purified by flash chromatography (eluent, EtOAc:CH_3_OH 94:6) to give 8 a white solid (1.17 g, 42% yield). Mp 90–91 °C. ^1^H NMR (600 MHz, CDCl_3_) δ 6.74 (dq, J = 15.4, 7.9 Hz, 1H), 6.01 (dd, J = 15.2, 3.6 Hz, 1H), 5.84 (br s, 1H), 4.18–4.06 (m, 4H), 3.15 (t, J = 6.3 Hz, 2H), 2.73 (dd, J = 22.7, 7.7 Hz, 2H), 1.86–1.76 (m, 1H), 1.33 (t, J = 7.1 Hz, 6H), 0.93 (d, J = 6.7 Hz, 6H). ^13^C NMR (151 MHz, CDCl_3_) δ 165.0 (d, ^4^J_C-P_ = 2.6 Hz), 132.5 (d, ^3^J_C-P_ = 11.1 Hz), 128.5 (d, ^2^J_C-P_ = 13.6 Hz), 62.3 (d, ^2^J_C-P_ = 6.8 Hz), 46.9, 30.10 (d, ^1^J_C-P_ = 139.1 Hz), 28.5, 20.1, 16.4 (d, ^3^J_C-P_ = 5.9 Hz). HRMS ESI m/z: calc. for C_12_H_25_NO_4_P^+^ = 278.15157, found = 278.15160 [M + H]^+^; calc. for C_12_H_24_NNaO_4_P^+^ = 300.13352, found = 300.13348 [M + Na]^+^; calc. for C_12_H_24_KNO_4_P^+^ = 316.10745, found = 316.10738 [M + K]^+^.

3.1.6. N-(Adamantan-1-yl)-1-(4-bromophenyl)-1H-1,2,3-triazole-4-carboxamide (39)

Prepared from 40 and 1-aminoadamantane. Reaction time: 20 h. Purified by flash chromatography (eluent CH_2_Cl_2_) to give a white solid (86% yield). Mp > 270 °C. ^1^H NMR (400 MHz, CDCl_3_): δ 8.35 (s, 1H), 7.62 (d, J = 8.7 Hz, 2H), 7.56 (d, J = 8.7 Hz, 2H), 6.88 (s, 1H), 2.09 (s, 10H), 1.75–1.61 (m, 5H). ^13^C NMR (100 MHz, CDCl_3_): δ 158.6, 145.3, 135.6, 133.1, 123.1, 123.0, 122.1, 52.4, 41.7, 36.3, 29.5. HRMS ESI m/z: calc. for C_19_H_21_BrN_4_NaO^+^ = 423.07909–425.07705, found 423.07960–425.07762 [M + Na]^+^.

3.1.7. General Procedure for the Synthesis of Compounds 11–26

A suspension of LiOH monohydrate (2.20 mmol) was added to a solution of aldehyde 1–5 (1.10 mmol) and the appropriate phosphonate 6–8 (0.55 mmol) in dry THF (5 mL). The reaction mixture was stirred under a N_2_ atmosphere at reflux temperature for 3–6 h, then filtered under vacuum and evaporated to dryness. The residue was dissolved in EtOAc and the organic solution was washed with brine, dried over anhydrous Na_2_SO_4_, filtered and evaporated. The crude product was purified by flash chromatography and/or trituration by an appropriate solvent.

(2E,4E)-N-Isobutyl-5-phenylpenta-2,4-dienamide (11) [51]

Prepared from phenylacetaldehyde and phosphonate 8. The product was purified by trituration with Et_2_O affording a white solid (0.16 g, 79% yield). Mp 109–112 °C. ^1^H NMR (400 MHz, CDCl_3_) δ 7.25–7.09 (m, 6H), 6.19–6.05 (m, 2H), 5.72 (d, J = 14.9 Hz, 1H), 5.45 (bs, 1H), 3.41 (d, J = 4.2 Hz, 2H), 3.09–3.08 (m, 2H), 1.76–1.69 (m, 1H), 0.85 (d, J = 5.5 Hz, 6H). ^13^C NMR (100 MHz, CDCl_3_) δ 166.3, 140.6, 140.5, 139.1, 129.4, 128.6, 128.5, 126.3, 122.9, 77.3, 77.0, 76.7, 46.9, 39.1, 28.6, 20.1. MS (ESI): m/z 244 [M + H]^+^ (90), 266 [M + Na]^+^ (70). Mp 109–112 °C.

(2E,4E)-N-(Adamantan-1-yl)-5-phenylpenta-2,4-dienamide (12)

Prepared from benzaldehyde and phosphonate 6. The product was purified by flash chromatography (eluent, PE:EtOAc 3:1) affording a yellowish solid (0.17 g, 95% yield). Mp 110–113 °C. ^1^H NMR (400 MHz, CDCl_3_): δ 7.40–7.15 (m, 6H), 6.75 (m, 2H), 5.87 (d, J = 14.8 Hz, 1H), 5.41 (bs, 1H), 2.00 (s, 9H), 1.63 (s, 6H). ^13^C NMR (100 MHz, CDCl_3_): δ 165.4, 140.3, 138.8, 136.4, 128.7, 128.6, 127.0, 126.4, 125.3, 52.3, 41.7, 36.6, 29.5. HRMS ESI m/z: calc. for C_21_H_26_NO^+^ = 308.20089, found = 308.20088 [M + H]^+^; calc. for C_21_H_25_NNaO^+^ = 330.18284, found = 330.18285 [M + Na]^+^; calc. for C_21_H_25_KNO^+^ = 346.15677, found = 346.15688 [M + K]^+^.

(2E,4E)-N-(3-Hydroxyadamantan-1-yl)-5-phenylpenta-2,4-dienamide (13)

Prepared from benzaldehyde and phosphonate 7. The product was purified by flash chromatography (eluent, PE:EtOAc 1:2) affording a cream-white solid (0.17 g, 94% yield). Mp 227–230 °C. ^1^H NMR (400 MHz, CD_3_OD): δ 7.42 (d, J = 8.0 Hz, 2H), 7.27 (t, J = 8.0 Hz, 2H), 7.32–7.13 (m, 1H), 6.92–6.79 (m, 3H), 6.06 (d, J = 16.0 Hz, 1H), 2.18–1.49 (m, 14H). ^13^C NMR (100 MHz, CD_3_OD): δ 166.6, 139.8, 138.6, 136.5, 128.4, 128.3, 126.6, 126.3, 125.1, 68.2, 54.0, 48.1, 43.6, 39.8, 34.8, 30.7. HRMS ESI m/z: calc. for C_21_H_26_NO_2_^+^ = 324.19581, found 324.19673 [M + H]^+^; calc. for C_21_H_25_NNaO_2_^+^ = 346.17775, found 346.17816 [M + Na]^+^; calc. for C_42_H_50_N_2_NaO_4_^+^ = 669.36628, found = 669.36745 [2M + Na]^+^.

(2E,4E)-N-(Adamantan-1-yl)-5-(naphthalen-2-yl)penta-2,4-dienamide (14)

Prepared from 2-naphthaldehyde and phosphonate 6. The product was purified by trituration with Et_2_O to give a white solid (0.15 g, 97% yield). Mp 84–88 °C. ^1^H NMR (400 MHz, CDCl_3_) δ 7.70–7.62 (m, 4H), 7.49 (d, J = 8.6 Hz, 1H), 7.39–7.28 (m, 3H), 6.94–6.79 (m, 2H), 5.92 (d, J = 14.9 Hz, 1H), 5.54 (s, 1H), 2.03 (s, 10H), 1.63 (s, 5H). ^13^C NMR (100 MHz, CDCl3) δ 165.3, 140.0, 138.7, 133.9, 133.5, 133.4, 128.3, 128.2, 127.7, 126.8, 126.5, 126.4, 125.8, 123.3, 52.1, 41.7, 36.4, 29.5. HRMS ESI m/z: calc. for C_25_H_28_NO^+^ = 358.21654, found = 358.21655 [M + H]^+^; calc. for C_25_H_27_NNaO^+^ = 380.19849, found = 380.19848 [M + Na]^+^; calc. for C_25_H_27_KNO^+^ = 396.17242, found = 396.17258 [M + K]^+^.

(2E,4E)-N-Isobutyl-5-(naphthalen-2-yl)penta-2,4-dienamide (15)

Prepared from 2-naphthaldehyde and phosphonate 8. The product was purified by trituration with Et_2_O to give a white solid (97% yield). Mp 179–182 °C. ^1^H NMR (400 MHz, CDCl_3_) δ 7.76–7.63 (m, 4H), 7.54 (d, J = 8.6 Hz, 1H), 7.44–7.32 (m, 3H), 6.99–6.83 (m, 2H), 5.97 (d, J = 14.9 Hz, 1H), 5.77 (bs, 1H), 3.14 (t, J = 6.4 Hz, 2H), 1.77 (septd, J = 6.7 Hz, 1H), 0.89 (s, 3H), 0.88 (s, 3H). ^13^C NMR (100 MHz, CDCl_3_) δ 166.2, 140.8, 139.1, 133.8, 133.5, 133.5, 128.4, 128.2, 127.8, 127.7, 126.7, 126.5, 126.4, 124.2, 123.3, 47.1, 28.7, 20.2. HRMS ESI m/z: calc. for C_19_H_22_NO^+^ = 280.16959, found = 280.16940 [M + H]^+^; calc. for C_19_H_21_NNaO^+^ = 302.15154, found = 302.15128 [M + Na]^+^; calc. for C_19_H_21_KNO^+^ = 318.12547, found = 318.12535 [M + K]^+^.

(2E,4E)-5-([1,1′-Biphenyl]-3-yl)-N-isobutylpenta-2,4-dienamide (16)

Prepared starting from biphenyl-4-carboxaldehyde and phosphonate 8. The product was purified by trituration with Et_2_O to give a white solid (0.12 g, 72% yield). Mp 226–228 °C. ^1^H NMR (400 MHz, CDCl_3_) δ 7.44–7.27 (m, 10H), 6.84–6.81 (m, 2H), 5.91 (d, J = 14.8 Hz, 1H), 5.46 (bs, 1H), 3.14 (t, J = 6.4 Hz, 2H), 1.77 (septd, J = 6.7 Hz, 1H), 0.89 (s, 3H), 0.87 (s, 3H). ^13^C NMR (100 MHz, CDCl3) δ 166.0, 141.3, 140.9, 140.4, 138.6, 135.4, 128.8, 127.5, 127.4, 127.4, 126.9, 126.4, 124.0, 47.0, 28.6, 20.1. HRMS ESI m/z: calc. for C_21_H_24_NO^+^ = 306.18524, found = 306.18505 [M + H]^+^; calc. for C_21_H_23_NNaO^+^ = 328.16719, found = 328.16698 [M + Na]^+^; calc. for C_21_H_23_KNO^+^ = 344.14112, found = 344.14090 [M + K]^+^.

(2E,4E)-5-([1,1′-Biphenyl]-4-yl)-N-(-adamantan-1-yl)penta-2,4-dienamide (17)

Prepared starting from biphenyl-4-carboxaldehyde and phosphonate 6. The product was purified by trituration with PE to give a yellowish solid (0.15 g, 69% yield). Mp 196–198 °C. ^1^H NMR (400 MHz, CDCl_3_) δ 7.46–7.25 (m, 10H), 6.78 (d, J = 4 Hz, 2H), 5.88 (d, J = 14.8 Hz, 1H), 5.44 (s, 1H), 2.02 (s, 10H), 1.64 (s, 5H). ^13^C NMR (100 MHz, CDCl3) δ 165.2, 141.2, 140.4, 140.0, 138.1, 135.5, 128.8, 127.5, 127.4, 127.3, 126.9, 126.5, 125.7, 52.1, 41.7, 36.4, 29.5.). HRMS ESI m/z: calc. for C_27_H_29_NNaO^+^ = 406.21414, found = 406.21455 [M + Na]^+^; calc. for C_54_H_58_N_2_NaO_2_^+^ = 789.43905, found = 789.43998 [2M + Na]^+^.

(E)-N-(Adamantan-1-yl)-5-(4-(pyridin-4-yl)phenyl)penta-2,4-dienamide (18)

Aldehyde 1 and phosphonate 6 were used as starting materials. The product was purified by trituration with PE to give a brown solid (0.20 g, 95% yield). Mp 227–229 °C. ^1^H NMR (400 MHz, CD_3_OD): δ 8.52 (d, J = 5.1 Hz, 2H), 7.73 (d, J = 8.2 Hz, 4H), 7.67 (d, J = 5.1 Hz, 2H), 7.59 (d, J = 8.05 Hz, 2H), 7.22–7.16 (m, 1H), 7.02–6.86 (m, 2H), 6.12 (d, J = 14.9 Hz, 1H), 2.04 (s, 9H), 1.69 (s, 6H). ^13^C NMR (100 MHz, DMSO-d6): δ 164.4, 150.8, 146.7, 138.6, 137.8, 137.2, 137.1, 128.2, 128.1, 127.6, 121.5, 51.4, 41.5, 36.5, 29.3. HRMS ESI m/z: calc. for C_26_H_29_N_2_O^+^ = 385.22744, found 385.22799 [M + H]^+^.

(2E,4E)-N-Isobutyl-5-(3-phenoxyphenyl)penta-2,4-dienamide (19)

3-Phenoxybenzaldehyde and phosphonate 8 were used as starting materials. The product was purified by flash chromatography (eluent, PE:EtOAc 3:1) to give a white solid (0.21 g, 97% yield. Mp 133–135 °C. ^1^H NMR (400 MHz, CDCl_3_) δ 7.29–7.19 (m, 5H), 7.10–7.02 (m, 3H), 6.95–6.84 (m, 2H), 6.73–6.71 (m, 2H), 5.90 (d, J = 14.8 Hz, 1H), 5.58 (bs, 1H), 3.12 (t, J = 6.0 Hz, 2H), 1.78–1.72 (m, 1H), 0.87 (d, J = 6.5 Hz, 6H). ^13^C NMR (100 MHz, CDCl_3_) δ 166.0, 157.6, 157.0, 140.2, 138.2, 138.1, 130.0, 129.8, 127.2, 125.0, 123.4, 122.2, 119.1, 118.9, 116.9, 47.1, 28.6, 20.1. HRMS ESI m/z: calc. for C_21_H_24_NO_2_^+^ = 322.18016, found = 322.17974 [M + H]^+^; calc. for C_21_H_23_NNaO_2_^+^ = 344.16210, found = 344.16168 [M + Na]^+^; calc. for C_21_H_23_KNO_2_^+^ = 360.13604, found = 360.13576 [M + K]^+^.

(2E,4E)-N-(Adamantan-1-yl)-5-(3-phenoxyphenyl)penta-2,4-dienamide (20)

3-Phenoxybenzaldehyde and phosphonate 6 were used as starting materials. The product was purified by flash chromatography (eluent, PE:EtOAc 2:1) to give a white solid (0.21 g, 76% yield). Mp 174–178 °C. ^1^H NMR (400 MHz, CDCl_3_) δ 7.29–7.19 (m, 5H), 7.10–7.02 (m, 3H), 6.95–6.84 (m, 2H), 6.71–6.70 (m, 2H), 5.81 (d, J = 14.7 Hz, 1H), 5.13 (bs, 1H), 1.98 (s, 10H), 1.62 (s, 5H). ^13^C NMR (100 MHz, CDCl_3_) δ 165.0, 157.6, 157.0, 139.7, 138.4, 137.8, 130.0, 129.8, 127.2, 126.1, 123.4, 122.1, 119.0, 118.9, 116.8, 52.1, 41.7, 36.4, 29.5. ESI m/z: calc. for C_27_H_30_NO_2_^+^ = 400.22711, found = 400.22707 [M + H]^+^; calc. for C_27_H_29_NNaO_2_^+^ = 422.20905, found = 422.20901 [M + Na]^+^; calc. for C_27_H_29_KNO_2_^+^ = 438.18299, found = 438.18296 [M + K]^+^.

(2E,4E)-N-Isobutyl-5-(3-(pentyloxy)phenyl)penta-2,4-dienamide (21)

Prepared from aldehyde 2 and phosphonate 8. The product was purified by flash chromatography (eluent, PE:EtOAc 2:1) to give a white solid (0.12 g, 77% yield). Mp 80–84 °C. ^1^H NMR (400 MHz, CDCl_3_) δ 7.31 (dd, J = 14.9, 10.4 Hz, 1H), 7.03 (t, J = 7.9 Hz, 1H), 6.91 (t, J = 5.6 Hz, 1H), 6.84 (d, J = 7.6 Hz, 1H),6.78–6.63 (m, 4H), 6.08 (d, J = 14.9 Hz, 1H), 3.77 (t, J = 6.5 Hz, 2H), 3.60 (bs, 1H), 3.07 (t, J = 6.3 Hz, 2H), 1.84–1.67 (m, 2H), 1.64–1.59 (m, 2H), 1.39–1.18 (m, 4H), 0.84–0.79 (m, 9H). ^13^C NMR (100 MHz, CDCl_3_) δ 166.5, 159.4, 140.2, 138.7, 137.6, 129.6, 126.7, 124.9, 119.3, 114.8, 112.8, 67.8, 47.1, 29.0, 28.9, 28.6, 22.4, 20.3, 20.2, 13.9. HRMS ESI m/z: calc. for C_20_H_30_NO_2_^+^ = 316.22711, found = 316.22678 [M + H]^+^; calc. for C_20_H_29_NNaO_2_^+^ = 338.20905, found = 338.20872 [M + Na]^+^; calc. for C_20_H_29_KNO_2_^+^ = 354.18299, found = 354.18275 [M + K]^+^.

(2E,4E)-N-(Adamantan-1-yl)-5-(3-(cinnamyloxy)phenyl)penta-2,4-dienamide (22)

Aldehyde 3 and phosphonate 6 were used as starting materials. The product was purified by flash chromatography (eluent, PE:EtOAc 3:1) to give a white solid (0.21 g, 85% yield). Mp 160–161 °C. ^1^H NMR (400 MHz, CDCl_3_) δ 7.34 (d, J = 7.3 Hz, 1H), 7.27–7.14 (m, 5H), 6.97–6.94 (m, 2H), 6.80 (dd, J = 8.1, 2.0 Hz, 1H), 6.74–6.72 (m, 1H), 6.66 (d, J = 16.0 Hz, 1H), 6.33 (dt, J = 15.9, 5.7 Hz, 1H), 5.87 (d, J = 14.8 Hz, 1H), 5.43 (bs, 1H), 4.61 (d, J = 5.7 Hz, 2H), 2.01 (s, 10H), 1.63 (s, 5H). ^13^C NMR (100 MHz, CDCl_3_) δ 165.2, 159.1, 139.9, 138.5, 136.4, 133.1, 129.7, 128.6, 128.0, 126.9, 126.6, 125.8, 124.3, 119.9, 115.0, 113.2, 68.7, 52.1, 41.7, 36.4, 29.5. HRMS ESI m/z: calc. for C_30_H_34_NO_2_^+^ = 440.25841, found = 440.25840 [M + H]^+^; calc. for C_30_H_33_NNaO_2_^+^ = 462.24035, found = 462.24019 [M + Na]^+^; calc. for C_30_H_33_KNO_2_^+^ = 478.21429, found = 478.21416 [M + K]^+^.

(2E,4E)-5-(3-(Cinnamyloxy)phenyl)-N-isobutylpenta-2,4-dienamide (23)

Aldehyde 3 and phosphonate 8 were used as starting materials. The product was purified by flash chromatography (eluent, PE:EtOAc 2:1) to give a white solid (0.14 g, 69% yield). Mp 131–133 °C. ^1^H NMR (400 MHz, CDCl_3_) δ 7.36–7.30 (m, 3H), 7.25 (t, J = 7.4 Hz, 1H), 7.20–7.12 (m, 2H), 6.96–6.93 (m, 2H), 6.81–6.74 (m, 3H), 6.65 (d, J = 16.0 Hz, 1H), 6.32 (dt, J = 15.9, 5.7 Hz, 1H), 6.21 (bs, 1H), 6.00 (d, J = 14.8 Hz, 1H), 4.58 (d, J = 5.7 Hz, 2H), 3.12 (t, J = 6.4 Hz, 2H), 1.85–1.68 (m, 1H), 0.88 (d, J = 6.7 Hz, 6H). ^13^C NMR (100 MHz, CDCl_3_) δ 166.3, 158.9, 140.5, 138.8, 137.9, 136.4, 133.1, 129.8, 128.6, 128.0, 126.9, 126.6, 124.7, 124.3, 119.8, 115.1, 113.2, 68.7, 47.1, 28.7, 20.2. HRMS ESI m/z: calc. for C_24_H_28_NO_2_^+^ = 362.21146, found = 362.21126 [M + H]^+^; calc. for C_24_H_27_NNaO_2_^+^ = 384.19340, found = 384.19328 [M + Na]^+^; calc. for C_24_H_27_KNO_2_^+^ = 400.16734, found = 400.16738 [M + K]^+^.

(2E,4E)-N-isobutyl-5-(3-((3-methylbut-2-en-1-yl)oxy)phenyl)penta-2,4-dienamide (24)

Prepared starting from aldehyde 5 and phosphonate 8. The product was purified by flash chromatography (eluent, PE:EtOAc 2:1) to give a white solid (0.11 g, 65% yield). Mp 103–104 °C. ^1^H NMR (400 MHz, CDCl_3_) δ 7.32–7.28 (m, 1H), 7.20–7.16 (m, 2H), 6.96 (d, J = 7.6, 1H), 6.92 (bs, 1H), 6.79–6.75 (m, 3H), 5.88 (d, J = 14.9 Hz, 1H), 5.43 (bs, 1H), 4.46 (d, J = 6.6 Hz, 2H), 3.13 (t, J = 6.3 Hz, 2H), 1.79–1.74 (m, 4H), 1.69 (s, 3H), 0.88 (d, J = 6.6 Hz, 6H). ^13^C NMR (100 MHz, CDCl_3_) δ 166.0, 159.2, 140.8, 139.0, 137.7, 129.7, 126.6, 124.1, 119.6, 119.5, 115.1, 112.9, 64.8, 47.2, 28.6, 25.8, 20.1, 18.2. HRMS ESI m/z: calc. for C_20_H_28_NO_2_^+^ = 314.21146, found = 314.21134 [M + H]^+^; calc. for C_20_H_27_NNaO_2_^+^ = 336.19340, found = 336.19329 [M + Na]^+^; calc. for C_20_H_27_KNO_2_^+^ = 352.16734, found = 352.16730 [M + K]^+^.

(2E,4E)-5-(3-(Allyloxy)phenyl)-N-isobutylpenta-2,4-dienamide (25)

Aldehyde 4 and phosphonate 8 were used as starting materials. The product was purified by flash chromatography (eluent, PE:EtOAc 3:1) to give a white solid (0.09 g, 57% yield). Mp 104–106 °C. ^1^H NMR (400 MHz, CDCl_3_) δ 7.34–7.28 (m, 1H), 7.20–7.15 (m, 1H), 6.97 (d, J = 7.6 Hz, 1H), 6.92 (s, 1H), 6.80–6.74 (m, 3H), 6.04- 5.97 (m, 1H), 5.90 (d, J = 14.9 Hz, 1H), 5.45 (bs, 1H), 5.35 (d, J = 17.3 Hz, 1H), 5.23 (d, J = 10.5 Hz, 1H), 4.49 (d, J = 5.2 Hz, 2H), 3.13 (t, J = 6.3 Hz, 2H), 1.76 (m, 1H), 0.88 (d, J = 6.6 Hz, 6H). ^13^C NMR (100 MHz, CDCl_3_) δ 158.8, 140.8, 139.0, 137.8, 137.7, 133.2, 129.7, 126.7, 124.1, 119.9, 117.7, 115.2, 113.1, 68.9, 47.1, 28.6, 20.1. HRMS ESI m/z: calc. for C_18_H_24_NO_2_^+^ = 286.18016, found = 286.18007 [M + H]^+^; calc. for C_18_H_23_NNaO_2_^+^ = 308.16210, found = 308.16189 [M + Na]^+^; calc. for C_18_H_23_KNO_2_^+^ = 324.13604, found = 324.13584 [M + K]^+^.

(2E,4E)-N-(Adamantan-1-yl)-5-(3-(allyloxy)phenyl)penta-2,4-dienamide (26)

Aldehyde 4 and phosphonate 6 were used as starting materials. The product was purified by flash chromatography (eluent, PE:EtOAc 3:1) to give a white solid (0.08g, 40% yield). ^1^H NMR (400 MHz, CDCl_3_) δ 7.25–7.19 (m, 1H), 7.14 (pseudo-t, J = 8.0 Hz, 1H), 6.94 (d, J = 7.61 Hz, 1H), 6.88 (s, 1H), 6.76–6.70 (m, 2H), 6.02–5.92 (m, 1H), 5.85 (d, J = 14.8 Hz, 1H), 5.35–5.31 (m, 2H), 5.20 (d, J = 10.5 Hz, 1H), 4.46 (d, J = 5.2 Hz, 2H), 1.99 (s, 10H), 1.61 (s, 5H). ^13^C NMR (100 MHz, CDCl_3_) δ 165.1, 158.9, 139.9, 138.4, 137.9, 133.2, 129.6, 126.8, 125.8, 119.8, 117.7, 115.0, 113.0, 68.8, 52.1, 41.7, 36.4, 29.7. HRMS ESI m/z: calc. for C_24_H_30_NO_2_^+^ = 364.22711, found = 364.22713 [M + H]^+^; calc. for C_24_H_29_NNaO_2_^+^ = 386.20905, found = 386.20902 [M + Na]^+^; calc. for C_24_H_29_KNO_2_^+^ = 402.18299, found = 402.18302 [M + K]^+^.

3.1.8. General Procedure for the Preparation of Amides 27, 28 and 30–34

A suspension of 1-aminoadamantane or 3-amine-1-adamantanol (4.0 mmol) in CH_2_Cl_2_ (15 mL) was added to a solution of cinnamic acid or acid 40 (2.0 mmol), EDC (0.8 g, 4.0 mmol) and HOBt (0.3 g, 2.4 mmol) in CH_2_Cl_2_ (20 mL). The mixture was stirred at room temperature until completion of the reaction, then washed with a saturated aqueous solution of Na_2_CO_3_, dried over anhydrous Na_2_SO_4_, filtered and evaporated. The crude product was purified as detailed below by using the appropriate eluent or by trituration/recrystallization from the appropriate solvent.

N-(Adamantan-1-yl)cinnamamide (27) [52]

Reaction time: 6 h. Purified by flash chromatography on silica gel. Eluent: CH_2_Cl_2_. White solid which was recrystallized from MeOH. Yield: 89%. Mp 220–221 °C (lit. [52] 214–215 °C). ^1^H NMR (400 MHz, CDCl_3_): δ 7.48 (d, J = 15.5 Hz, 1H), 7.43–7.21 (m, 5H), 6.27 (d, J = 15.5 Hz, 1H), 5.31 (bs, 1H), 2.02 (s, 10H), 1.64 (s, 5H). ^13^C NMR (100 MHz, CDCl_3_): δ 165.1, 140.0, 135.1, 129.5, 128.7, 127.7, 122.5, 52.2, 41.7, 36.4, 29.5. HRMS ESI m/z: calc. for C_19_H_24_NO^+^ = 282.18524, found = 282.18573 [M + H]^+^; calc. for C_19_H_23_NNaO^+^ = 304.16719, found = 304.16764 [M + Na]^+^; calc. for C_38_H_47_N_2_O_2_^+^ = 563.36321, found = 563.36457 [2M + H]^+^; calc. for C_38_H_46_N_2_NaO_2_^+^ = 585.34515, found = 585.34640 [2M + Na]^+^.

N-(3-Hydroxy)adamant-1-yl cinnamamide (28)

Reaction time: 16 h. Purified by flash chromatography on silica gel. Eluent: PE:EtOAc 1:1. White solid which was recrystallized from MeOH. Yield: 35%. Mp 265–269 °C. ^1^H NMR (400 MHz, CD_3_OD): δ 7.55–7.18 (m, 5H), 7.37 (d, J = 15.7 Hz, 1H), 6.54 (d, J = 15.7 Hz, 1H), 4.76 (s, 1H), 2.18 (s, 1H), 2.03–1.41 (m, 14H). ^13^C NMR (100 MHz, DMSO-d6): δ 164.5, 138.7, 135.6, 129.7, 129.4, 127.8, 124.2, 67.7, 53.9, 49.5, 44.7, 35.4, 30.6. ESI m/z: calc. for C_19_H_24_NO_2_^+^ = 298.18016, found = 298.18045 [M + H]^+^; calc. for C_19_H_23_NNaO_2_^+^ = 320.16210, found = 320.16241 [M + Na]^+^; calc. for C_38_H_46_N_2_NaO_4_^+^ = 617.33498, found = 617.33620 [2M + Na]^+^.

(E)-N-(A-adamantan-1-yl)-3-(4-(pyridin-4-yl)phenyl)acrylamide (29)

Reaction time: 16 h. Purified by flash chromatography on silica gel. Eluent: PE:Et_2_O 1:4. White solid. Yield 54%. Mp 227–229 °C. ^1^H NMR (400 MHz, CD_3_OD): δ 8.52 (d, J = 5.5 Hz, 2H), 7.87–7.55 (m, 6H), 7.41 (d, J = 15.7 Hz, 1H), 6.63 (d, J = 15.7 Hz, 1H), 2.04 (s, 10H), 1.69 (s, 5H). ^13^C NMR (100 MHz, DMSO-d6): δ 164.4, 150.8, 146.7, 138.0, 137.4, 136.6, 128.6, 127.8, 125.2, 121.5, 51.4, 41.5, 36.5, 29.3. HRMS ESI m/z: calc. for C_24_H_27_N_2_O^+^ = 359.21179, found = 359.21164 [M + H]^+^.

(E)-N-(Adamantan-1-yl)-3-(4-bromophenyl)acrylamide (30) [53]

Reaction time: 16 h. Yellow solid which was purified by trituration with PE. Yield: 93%. Mp 232–233 °C. ^1^H NMR (400 MHz, CD_3_OD): δ 7.42–7.39 (m, 3H), 7.27–7.25 (m, 2H), 6.25 (d, J = 15.5 Hz, 1H), 5.31 (bs, 1H), 2.01 (s, 10H), 1.64 (s, 5 H). ^13^C NMR (100 MHz, CDCl_3_): δ 164.5, 138.9, 134.0, 132.0, 129.1, 123.5, 122.9, 52.3, 41.7, 36.3, 29.5. ESI m/z: calc. for C_19_H_23_BrNO^+^ = 360.09575–362.09371, found = 360.09631–362.09438 [M + H]^+^; calc. for C_19_H_22_BrNNaO^+^ = 382.07770–384.07565, found = 382.07824–384.07619 [M + Na]^+^; calc. for C_38_H_44_Br_2_N_2_NaO_2_^+^ = 743.16413, found = 743.16584 (100) [2M + Na]^+^.

(E)-3-([1,1′-Biphenyl]-4-yl)-N-(adamantan-1-yl)acrylamide (31)

Reaction time: 5 h. Yellow solid which was purified by trituration with PE. Yield: 99%. Mp 171–173 °C. ^1^H NMR (400 MHz, CD_3_OD): δ 7.59–7.53 (m, 6H), 7.42–7.25 (m, 4H), 6.58 (d, J = 15.7 Hz, 1H), 2.04 (s, 10H), 1.68 (s, 5H). ^13^C NMR (100 MHz, DMSO-d6): δ 164.5, 141.2, 139.9, 137.7, 134.79, 129.5, 128.5, 128.2, 127.6, 127.1, 124.3, 51.4, 41.5, 36.5, 29.3. HRMS ESI m/z: calc. for C_25_H_28_NO^+^ = 358.21654, found = 358.21612 [M + H]^+^; calc. for C_25_H_27_NNaO^+^ = 380.19849, found = 380.19808 [M + Na]^+^; calc. for C_25_H_27_KNO^+^ = 396.17242, found = 396.17212 [M + K]^+^.

(E)-3-(1,1′-Biphenyl-4-yl)-N-(3-hydroxyadamantan-1-yl)acrylamide (32)

Reaction time: 16 h. Purified by recrystallization from MeOH. White crystals. Yield: 94%. Mp 246 °C. ^1^H NMR (400 MHz, DMSO-d6): δ 7.65–7.52 (m, 6H), 7.42–7.27 (m, 4H), 6.64 (d, J = 15.8 Hz, 1H), 4.42 (s, 1H), 2.08 (s, 2H), 1.86–1.76 (m, 6H), 1.52- 1.36 (m, 6H). ^13^C NMR (100 MHz, DMSO-d6): δ 164.6, 141.2, 139.9, 137.8, 134.7, 129.5, 128.5, 128.2, 127.6, 127.1, 124.2, 67.8, 54.0, 50.0, 44.7, 35.4, 30.6. HRMS ESI m/z: calc. for C_25_H_28_NO_2_^+^ = 374.21146, found = 374.21145 [M + H]^+^; calc. for C_25_H_27_NNaO_2_^+^ = 396.19340, found = 396.19335 [M + Na]^+^; calc. for C_25_H_27_KNO_2_^+^ = 412.16734, found = 412.16745 [M + K]^+^.

(E)-N-(Adamantan-1-yl)-3-(3-hydroxyphenyl)acrylamide (33)

Reaction time: 6 h. Purified by flash chromatography (eluent, PE:EtOAc 1:1). White solid. Yield: 73%. Mp 230–232 °C. ^1^H NMR (400 MHz, CD_3_OD): δ 7.33 (d, J = 15.7 Hz, 1H), 7.11 (pseudo-t, J = 7.9 Hz, 1H), 6.92 (d, J = 7.9 Hz, 1H), 6.87 (s, 1H), 6.71 (dd, J_1_ = 8.1, J_2_ = 2.3 Hz, 1H), 6.48 (d, J = 15.7 Hz, 1H), 4.70 (s, 1H), 2.02 (s, 10H), 1.67 (s, 5H). ^13^C NMR (100 MHz, DMSO-d6): δ 164.6, 158.1, 138.4, 137.0, 130.3, 124.0, 119.0, 116.9, 114.1, 51.3, 41.5, 36.5, 29.3. HRMS ESI m/z: calc. for C_19_H_24_NO_2_^+^ = 298.18016, found = 298.18009 [M + H]^+^; calc. for C_19_H_23_NNaO_2_^+^ = 320.16210, found = 320.16205 [M + Na]^+^; calc. for C_19_H_23_KNO_2_^+^ = 336.13604, found = 336.13602 [M + K]^+^.

(E)-N-(3-Hydroxyadamantan-1-yl)-3-(3-hydroxyphenyl)acrylamide (34)

Purified by flash chromatography (eluent, CH_2_Cl_2_:MeOH 98:2). White solid. Yield: 27%. Mp 238 °C. ^1^H NMR (400 MHz, CD_3_OD): δ 7.29 (d, J = 15.7 Hz, 1H), 7.11 (pt, J = 7.9 Hz, 1H), 6.92 (d, J = 7.7 Hz, 1H), 6.71 (dd, J_1_ = 2.2, J_2_ = 7.9 Hz, 1H), 6.48 (d, J = 15.7 Hz, 1H), 2.18 (s, 2H), 1.97–1.93 (m, 6H), 1.67–1.49 (m, 6H). ^13^C NMR (100 MHz, DMSO-d6): δ 164.6, 158.1, 138.5, 136.9, 130.3, 123.9, 119.0, 116.9, 114.1, 67.7, 53.9, 49.6, 44.7, 35.3, 30.6. HRMS ESI m/z: calc. for C_19_H_24_NO_3_^+^ = 314.17507, found = 314.17507 [M + H]^+^; calc. for C_19_H_23_NNaO_3_^+^ = 336.15701, found = 336.15696 [M + Na]^+^; calc. for C_19_H_25_NNaO_4_^+^ = 354.16758, found = 354.16766 [M + Na + H_2_O]^+^.

3.1.9. General Procedure for the Preparation of 35 and 36 by Horner–Wadsworth–Emmons Condensation

Biphenyl-4-carboxaldehyde or 3-hydroxybenzaldehyde (5.5 mmol) was added to a solution of triethyl phosponacetate (1.3 mL, 6.6 mmol) and finely ground K_2_CO_3_ (2.3 g, 16.5 mmol) in absolute EtOH (30 mL) and the reaction mixture was refluxed for 2 h. After cooling, the solvent was removed under reduced pressure and the residue was solubilized in Et_2_O. The organic solution was washed with brine, dried over anhydrous Na_2_SO_4_, filtered and evaporated to give a solid residue.

Ethyl (E)-3-([1,1′-Biphenyl]-4-yl)acrylate (35) [54]

Obtained as a pure white solid which did not need further purification. Yield: 99%. Mp 53–55 °C. ^1^H NMR (600 MHz, CDCl_3_) δ 7.75 (d, J = 16.0 Hz, 1H), 7.67–7.61 (m, 6H), 7.48 (t, J = 7.6 Hz, 2H), 7.40 (t, J = 7.4 Hz, 1H), 6.50 (d, J = 16.0 Hz, 1H), 4.31 (q, J = 7.1 Hz, 2H), 1.38 (t, J = 7.1 Hz, 3H). ^13^C NMR (151 MHz, CDCl_3_) δ 167.1, 144.1, 143.0, 140.2, 133.5, 128.9, 128.6, 127.8, 127.6, 127.1, 118.2, 60.5, 14.4. HRMS ESI m/z: calc. for C_17_H_16_NaO_2_^+^ = 275.10425, found 275.10437 [M + Na]^+^.

Ethyl (E)-3-(3-Hydroxyphenyl)acrylate (36) [55]

The solid residue was recrystallized from hexane–benzene mixture to obtain a lightly pink solid. Yield: 98%. Mp 87–90 °C. ^1^H NMR (600 MHz, CD_3_OD) δ 7.60 (d, J = 16.0 Hz, 1H), 7.22 (t, J = 7.9 Hz, 1H), 7.05 (d, J = 7.6 Hz, 1H), 7.02–7.00 (m, 1H), 6.85 (ddd, J = 8.1, 2.5, 0.9 Hz, 1H), 6.44 (d, J = 16.0 Hz, 1H), 4.24 (q, J = 7.1 Hz, 2H), 1.33 (t, J = 7.1 Hz, 3H). ^13^C NMR (151 MHz, CD_3_OD) δ 167.3, 157.7, 144.9, 135.6, 129.6, 119. 3, 117.5, 117.3, 113.9, 60.24 13.23. HRMS ESI m/z: calc. for C_11_H_12_NaO_3_^+^ = 215.06787, found 215.06814 [M + Na]^+^; calc. for C_22_H_24_NaO_6_^+^ = 407.14651, found 407.14700 [2M + Na]^+^.

3.1.10. General Procedure for the Preparation of Compounds 37 [56] and 38 by Alkaline Hydrolysis

A solution of 1 N NaOH (0.2 g, 5.3 mmol) was added to a solution of 35 or 36 (5.3 mmol) in EtOH (8 mL) and the reaction mixture was stirred at r.t. for 1–4 days. The pH was adjusted to 1 using 12 N HCl and the aqueous solution was extracted with EtOAc. The organic phase was washed with brine, dried over anhydrous Na_2_SO_4_, filtered and evaporated to give the expected acids 37 or 38 in a yield of 48% and 97%, respectively, as white solids which were used directly in the next step without further purification.

3.2. TRPA1 Assay

Assessment of the effects of compounds on rat TRPA1 were performed by continuously monitoring the elevation of intracellular Ca^2+^ concentration ([Ca^2+^]i) using the selective intracellular fluorescent probe for Ca^2+^ Fluo-4, as previously described [37]. Briefly, human embryonic kidney (HEK-293) cells, stably transfected with rat TRPA1 or not transfected, were cultured in EMEM with 2 mM glutamine, 1% non-essential amino acids, and 10% FBS and maintained at 37 °C with 5% CO_2_. TRPA1-HEK-293 cells stably express high levels of TRP transcripts, while these transcripts were virtually absent in wild-type HEK-293 cells as checked by real-time PCR. On the day of the experiment, the cells were loaded for 1 h in the dark at room temperature with Fluo-4 AM (4 μM in DMSO containing 0.02% Pluronic F-127). The cells were rinsed, resuspended in Tyrode’s solution (145 mM NaCl, 2.5 mM KCl, 1.5 mM CaCl_2_, 1.2 mM MgCl_2_, 10 mM D-glucose, and 10 mM HEPES, pH 7.4), and transferred to the quartz cuvette of a spectrofluorometer (PerkinElmer LS50B; λEX = 488 nm, λEM = 516 nm) equipped with a PTP-1 fluorescence Peltier system (PerkinElmer Life and Analytical Sciences, Waltham, MA, USA) under continuous stirring. Cell fluorescence before and after the addition of various concentrations of the tested compounds was measured, normalizing the effects against the response to ionomycin (4 μM). The values of the effect on [Ca^2+^]i in HEK-293 cells not transfected are used as a baseline and subtracted from the values obtained from transfected cells. Agonist efficacy was expressed as a percentage of the effect on [Ca^2+^]i observed with 100 μM allylisothiocyanate (AITC). The potency of the compounds (EC_50_ values) is determined as the concentration required to produce half-maximal increases in [Ca^2+^]i. Antagonist/desensitizing behavior is evaluated against the agonist of the TRPA1 analyzed by adding the compounds directly in the quartz cuvette 5 min before stimulation of cells with the agonist AITC (100 μM). The IC_50_ value is expressed as the concentration exerting a half-maximal inhibition of agonist effect, taking as 100% the effect on [Ca^2+^]i exerted by the agonist alone. Dose–response curve fitting (sigmoidal dose–response variable slope) and parameter estimation were performed with GraphPad Prism9 (GraphPad Software Inc., San Diego, CA, USA). All determinations were performed at least in triplicate.

3.3. Competitive Binding Assay

Binding assays at equilibrium were performed as previously reported [57]. Briefly, membranes from HEK-293 cells over-expressing the respective human recombinant CB1 receptor (B max = 5.7 pmol/mg protein) and human recombinant CB2 receptor (B max = 11.4 pmol/mg protein) were incubated with [^3^H]-CP-55,940 (0.4 nM/KD = 3.08 nM and 0.53 nM/KD = 5.89 nM, respectively, for CB1 and CB2 receptors) as the high-affinity ligand. Competition curves were performed by displacing [^3^H]-CP-55,940 with increasing concentration of compounds (0.01–10 μM). Nonspecific binding was defined by 10 μM of WIN55, 212–2 as the heterologous competitor (Ki values 8.8 nM and 0.89 nM, respectively, for CB1 and CB2 receptor). All compounds were tested following the procedure described by the manufacturer (PerkinElmer, Italy). Displacement curves were generated by incubating compounds with [^3^H]-CP-55,940 for 90 min at 30 °C. Ki values were calculated by applying the Cheng–Prusoff equation to the IC_50_ values (obtained by GraphPad PRISM 10.5.1 software) for the displacement of the bound radioligand by increasing concentrations of the test compound. Data represent the mean values for three separate experiments performed in duplicate and are expressed as the average ± SEM.

3.4. Computational Methods

Ligands were built with UCSF Chimera 1.17 [58] followed by initial energy minimization (EM) at the molecular mechanics level, using AM1-BC charges. The molecules were then fully optimized using the GAMESS 13R1.X program [59] at the Hartree–Fock level with the STO-3G basis set and subjected to HF/6-31G*/STO-3G single-point calculations to derive the partial atomic charges using the RESP procedure [60]. Docking studies were performed with AutoDock 4.2 [61] using homology models of rat TRPA1(rTRPA1) in both the activated state, already published [36], and in the closed form, using as templates hTRPA1 cryo-EM structures in complex with antagonists bound to different sites (PDB id: 7OR0 and 6WJ5) and following the same homology modeling protocol previously adopted [36]. Briefly, the modeller 10.4 program [62] has been used to build 50 homology models, selected on the basis of the DOPE score and Modeler Objective Function. Proteins and ligands were processed with AutoDock Tools (ADT) package version 1.5.6rc1 to merge non-polar hydrogens and calculate Gasteiger charges. For docking calculations, grids were used with a spacing of 0.375 Å and boxes with different dimensions to encompass either both sites Site1ago and Site2ago (80 × 60 × 80) and each ligand binding site named Site1ago (50 × 50 × 70), Site2ago (70 × 60 × 70), Site1ant (60 × 60 ×70) and Site2ant (60 × 60 × 80), generated using the program AutoGrid 4.2 included in the AutoDock 4.2 distribution, following the docking protocol already published [36]. Briefly, 100 docking poses were calculated for each docking run, using the Lamarckian genetic algorithm (LGA) and the following parameters: 100 individuals in a population with a maximum of 15 million energy evaluations and a maximum of 37,000 generations, followed by 300 iterations of Solis and Wets local search. To validate the docking protocol on this protein system, the experimentally solved antagonists were re-docked onto their respective structures, obtaining ligand rmsd values for 6WJ5 and 7OR0 of 1.26 Å and 1.1 Å, respectively. Moreover, the agonist from the 6X2J structure was cross-docked into the 6PQO structure with a rmsd of 1.3 Å. Flexibility was used for all rotatable bonds of docked ligands. Docking runs were carried out by either keeping the whole protein fixed or allowing the rotation of selected residues (Phe844, Phe843, Met847 and Phe870 for Site1ago and Thr877, Leu737, Ile953, Phe912 and Met915 for Site2ago/Site1ant). The representative complexes, selected on the basis of binding energy and cluster population, were then completed by addition of all hydrogen atoms, and energy-minimized (EM) with the Amber16 package [63], using the 14SB version of the AMBER force field for the protein and gaff parameters for the ligand. The UCSF Chimera 1.17 program was used to draw the figures.

3.5. In Vivo Studies

3.5.1. Animals

Male C57/BLJ (ENVIGO, Udine, Italy) mice weighing 25 g were housed three per cage under controlled illumination (12 h light/dark cycle; light on 6:00 a.m.) and room temperature 20–22 °C, humidity 55–60%, for 1 week before the commencement of experiments. Mouse food and tap water were available ad libitum. The experimental procedures were approved by the Animal Ethics Committee of University of Campania “L. Vanvitelli,” Naples. Animal care followed the Italian (D.L. 116/92) and European Commission (O.J. of E.C. L358/1 18/12/86) regulations on the protection of laboratory animals (MOH project: 806/2024). Every effort was made to reduce suffering and animal numbers.

3.5.2. Pain Models

Formalin model. Animals received formalin (1.25% in saline, 30 μL) on the dorsal surface of the right hind paw. Each mouse, randomly assigned to one of the experimental groups (N = 5–6), was placed in a plexiglass cage and allowed to move freely for 20 min. A mirror was placed at a 45° angle under the cage to allow full view of the hind paws. Lifting, favoring, licking, shaking, and flinching of the injected paw were recorded as nocifensive behavior [43]. Formalin injections induce a biphasic and stereotyped nociceptive behavior. A first brief phase (0–7 min) caused by a primary afferent nerve discharge is followed by a period of quiescence and then by a second phase (15–60 min) of prolonged tonic pain. The total duration of the nociceptive response was measured every 5 min for 1 h and expressed in minutes (mean ± S.E.M.).

Spared nerve injury (SNI). Mononeuropathy was induced according to the method of Decosterd and Woolf [31]. Mice were anesthetized by intraperitoneal injection of ketamine xylazine (60 mg/kg + 10 mg/kg, respectively). Once the right sciatic nerve was exposed, the tibial and common peroneal nerves were tied tightly with 7.0 silk threads and then sectioned distal to the node, leaving the sural nerve intact. Sham-operated mice were anesthetized, and the sciatic nerve was exposed at the same level but not sectioned. In the SNI test, the Von Frey test was used to measure tactile allodynia with a series of calibrated Von Frey nylon monofilaments (Semmes-Weinstein monofilaments, 2 Biological Instruments, Italy). Two weeks after surgery, mice were placed on the wire mesh surface of the test cage, and testing began after mice had acclimated to the new environment for 30 min before any measurements were taken. Monofilaments, starting with the 0.008 g monofilament, were applied perpendicularly to the plantar surface of each hind paw with a series of ascending forces (0.008, 0.02, 0.04, 0.07, 0.16, 0.40, 0.60, 1.0, 1.4, and 2.0 g). Each stimulus was applied for approximately 1 sec with a 5 sec interstimulus interval. The withdrawal responses evoked by each monofilament were obtained from five consecutive trials. Tactile allodynia was defined as a significant decrease in the withdrawal threshold following the application of von Frey hair. Each mouse was used as a control, and responses were measured both before and after surgery.

3.5.3. Statistical Analysis

Behavioral data were expressed as the mean ± S.E.M. Two-way ANOVA for repeated measures followed by Tukey’s post hoc test or Bonferroni’s test was used for comparison between groups. GraphPad Prism version 10 (GraphPad Software Inc., San Diego, CA, USA) was used in all statistical analyses. A probability < 0.05 was considered sufficient to reject the null hypothesis.

3.5.4. Treatments

The analgesic effect of compounds 30 and 17 was evaluated in formalin-induced and SNI pain models. Mice received the vehicle (10% DMSO) or test compounds at doses of 1, 2, or 3 μg/mouse (20 μL/intra-paw) 10 min before formalin injection. The TRPA1 antagonist AP18 (0.05 mg/kg, ip) was administered 20 min before intra-paw injections of 30 or 17. In the SNI model, mice were treated with the vehicle (10% DMSO) or compound 17 (0.5, 1, or 3 mg/kg).

4. Conclusions

Taking inspiration from natural products of the sanshool family, we designed, synthesized, and characterized new synthetic analogues acting as TRPA1 modulators. Several compounds displayed partial agonist/desensitizing profiles with low micromolar potency and were devoid of cannabimimetic properties. Among them, 17 and 30 emerged as the most promising molecules, showing consistent in vivo analgesic effects in both the formalin test and the spared nerve injury model. Importantly, 17 demonstrated a dual, dose-dependent mechanism of action, shifting from agonist activity to functional antagonism at higher doses, whereas 30 primarily acted as an antagonist in vivo. Our data indicate that these novel derivatives may represent valuable leads for the development of innovative analgesic strategies targeting TRPA1, with potential applications in both inflammatory and neuropathic pain. Further preclinical studies will be necessary to optimize their pharmacological profile, assess their safety, and refine their physicochemical properties.