Identification of Upland Rice Genotypes Resistant to Neck Blast Disease: A Systematic Review of Field and Greenhouse Studies

TL;DR

This review identifies upland rice genotypes resistant to neck blast disease and highlights the potential of genetic markers for breeding resistant varieties.

Contribution

The study systematically reviews field and greenhouse studies to identify resistant upland rice genotypes and genetic factors for neck blast disease.

Findings

Twenty genotypes showed moderate to high resistance to neck blast disease.

Three QTLs (qBFR4-1, qBl1, and qBl2) were associated with durable resistance.

Marker-assisted selection is highlighted as a promising approach for breeding resistant varieties.

Abstract

This systematic review synthesizes evidence on upland rice (Oryza sativa L.) genotypes resistant to neck blast disease caused by Magnaporthe oryzae, focusing on resistant lines, screening methods, and genetic factors underlying resistance. Empirical studies published in English between 1980 and 2025 were identified through searches of PubMed, ScienceDirect, Google Scholar, and grey literature, with final searches completed on 31 October 2025. Eligible studies evaluated upland rice under upland or rainfed conditions. The risk of bias was assessed using a customized framework adapted from the ROBINS-I tool, and findings were synthesized narratively due to substantial methodological heterogeneity. Six studies from Asia and Africa, encompassing 248 genotypes, met the inclusion criteria. Twenty genotypes—including Kahei, Barkhe 1032, Barkhe 1035, Barkhe 2014, several NERICA lines, and BC1F4 backcross derivatives—demonstrated moderate to high resistance based on the IRRI 0–9 Standard Evaluation System. Two studies reported quantitative trait loci (qBFR4-1, qBl1, and qBl2) associated with durable resistance, highlighting the potential of QTL-based breeding. Despite limitations related to small sample sizes, heterogeneous methodologies, and limited molecular characterization, particularly for neck blast-specific resistance, this review underscores the promise of marker-assisted selection. Future research should prioritize neck blast-focused QTL validation, expanded genomic screening, harmonized screening protocols, and multi-location field trials to confirm resistance durability and agronomic performance across diverse upland environments.

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