Characterization of an Indigenous Lytic Phage Targeting Multidrug-Resistant Salmonella enterica
Ebele Onuigbo, Paul Akpa, Anthony Attama, Stephen Emencheta, Emmanuel Eze, Chinonye Obeta
TL;DR
This paper describes a new lytic phage that can target and inhibit multidrug-resistant Salmonella strains found in wastewater.
Contribution
The study characterizes a novel indigenous phage with potential therapeutic use against multidrug-resistant Salmonella.
Findings
The phage showed high adsorption efficiency and a short latent period when targeting Salmonella strains.
The phage released 55 PFU/cell during replication, effectively inhibiting bacterial growth for 5 hours.
The phage performed best in alkaline conditions and temperatures below 50°C.
Abstract
Multidrug-resistant pathogens have prompted the use of lytic bacteriophages. An indigenous novel lytic bacteriophage against Salmonella enterica strains from environmental wastewater was isolated and characterized using phage survivability study, adsorption curve, one-step curve, optimal multiplicity of infection, and phage-killing assay. The Salmonella strains CP90 and CP23 isolated from the same source were biochemically and molecularly characterized. The Salmonella strains CP90 and CP23 had 96.24% and 97.18% pairwise identity respectively with S. enterica. Both were resistant to B-lactam Aminoglycosides, Penicillin, and Phenicol class of antibiotics. The phage performed better in an alkaline medium and below 50°C. About 80% of the phage had an adsorption rate of 12 min and a latent period of 20 min. About 55 PFU/cell of the phage was released during a single replication cycle,…
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Taxonomy
TopicsBacteriophages and microbial interactions · Salmonella and Campylobacter epidemiology · Vibrio bacteria research studies