# Development of a Specialized Method for Simultaneous Quantification of Functional Intestinal Metabolites by GC/MS-Based Metabolomics

## TL;DR

This paper introduces a new method to measure multiple intestinal metabolites at once using GC/MS, improving efficiency and accuracy in gut health research.

## Contribution

A novel GC/MS-based method with TMS derivatization enables simultaneous quantification of diverse intestinal metabolites in fecal samples.

## Key findings

- Trimethylsilyl derivatization allows GC/MS to quantify volatile SCFAs along with BAs, AAs, and sugars in fecal samples.
- The fecal matrix stabilizes SCFAs during the dry solidification process, preventing volatilization.
- The method successfully quantified 65 metabolites and validated biological correlations in mouse studies.

## Abstract

Intestinal metabolites produced by gut microbes play a significant role in host health. Due to their different chemical structures, they are often analyzed using multiple analyzers and methods, such as gas chromatography/mass spectrometry (GC/MS) for SCFAs and liquid chromatography/mass spectrometry (LC/MS) for bile acids (BAs), amino acids (AAs), and sugars. In this study, we aimed to develop a specialized method for the simultaneous determination of important intestinal metabolites, specifically addressing the main issue of SCFA volatilization during the dry solidification process. We discovered that these compounds can all be measured in fecal samples by GC/MS after trimethylsilyl (TMS) derivatization despite the expected volatility of SCFAs. Validating the results using SCFA standards suggested that the fecal matrix exerts a stabilizing effect. This method enabled the simultaneous quantification of 65 metabolites. For further validation in a biological context, a mouse study showed that high-MAC and high-fat diets increased SCFAs and BAs in feces, respectively, and showed a negative correlation between Alistipes and sugars, all consistent with previous studies. As a result, we successfully developed a specialized simultaneous quantification method for SCFAs, BAs, AAs, AA derivatives, and sugars in fecal samples using GC/MS-based metabolomics in conjunction with a TMS derivatization pretreatment process.