# Production Performance and Properties of Eggs from Hens Fed Diets Differing in Corn Grain Hardness, Vitamin A Supplementation Level, and Mineral Form

**Authors:** Kristina Kljak, Dora Zurak, Goran Kiš, Zlatko Janječić, Dalibor Bedeković, Helga Medić, Vasil Pirgozliev, Nives Marušić Radovčić

PMC · DOI: 10.3390/foods15040692 · 2026-02-13

## TL;DR

This study shows how changes in corn hardness, vitamin A levels, and mineral form affect egg production and quality in hens.

## Contribution

The study reveals how dietary factors modulate egg nutritional composition and oxidative stability in laying hens.

## Key findings

- Hard-type corn improves egg mass and feed conversion but reduces n-3 fatty acids and increases lipid oxidation susceptibility.
- Higher vitamin A levels increase egg weight and shell strength but reduce tocols and increase lipid oxidation markers.
- Trace mineral form has minor effects on performance and fatty acid profiles.

## Abstract

This study evaluated how corn grain hardness, vitamin A supplementation level, and trace mineral form influence production performance and egg properties in laying hens. In a 2 × 3 × 2 factorial design, 252 Lohmann Brown hens received diets containing soft- or hard-type corn hybrids; 5000, 10,000, or 20,000 IU/kg of vitamin A; and inorganic or organic trace minerals for 63 days. Hard-type corn increased daily egg mass, improved feed conversion ratio, and produced eggs with higher MUFAs and SFAs but lower PUFAs and n-3, resulting in a less favorable n6/n3 ratio, while also increasing susceptibility to Fe-induced lipid oxidation despite lower PUFAs. Increasing dietary vitamin A to 10,000–20,000 IU/kg increased egg weight and shell strength, linearly increased yolk retinol, and decreased tocols, with 20,000 IU/kg markedly increasing Fe-induced MDA formation without major changes in PUFAs. Trace mineral form had minor effects on performance and fatty acid profile. Overall, modest changes in laying hen diet, such as corn hybrid, vitamin A supplementation level, and trace mineral form, significantly modulated egg nutritional composition and oxidative stability. A high dietary vitamin A level may compromise the oxidative resilience of enriched eggs, while interactions of trace mineral form with corn hybrid and vitamin A suggest its potential modulatory role in lipid oxidation pathways.

## Linked entities

- **Chemicals:** vitamin A (PubChem CID 445354), Fe (PubChem CID 23925), MDA (PubChem CID 1614), retinol (PubChem CID 3840), tocols (PubChem CID 58866062)

## Full-text entities

- **Genes:** ALB (albumin) [NCBI Gene 396197], CAT (catalase) [NCBI Gene 423600]
- **Diseases:** injury to (MESH:D014947), inflammatory (MESH:D007249)
- **Chemicals:** TBARS (MESH:D017392), a (MESH:D001151), KCl (MESH:D011189), lysine (MESH:D008239), oleic acid (MESH:D019301), oxides (MESH:D010087), calcium (MESH:D002118), Mn (MESH:D008345), alpha-tocopherol (MESH:D024502), polypropylene (MESH:D011126), palmitic acid (MESH:D019308), DHA (MESH:D004281), Lipid (MESH:D008055), amino acid (MESH:D000596), sodium selenite (MESH:D018038), MUFA (MESH:D005229), 1,1,3,3-tetramethoxypropane (MESH:C041295), tocopherol (MESH:D024505), Fatty Acid (MESH:D005227), MDA (MESH:D008315), C18:3n6 (MESH:D017965), Se (MESH:D012643), trichloroacetic acid (MESH:D014238), starch (MESH:D013213), palmitoleic acid (MESH:C008757), Helium (MESH:D006371), bile salt (MESH:D001647), Tocol (MESH:C572520), C16:0 (-), copper sulfate (MESH:D019327), tocotrienols (MESH:D024508), PUFA (MESH:D005231), hexane (MESH:D006586), gamma-tocopherol (MESH:D024504), trans-oleic acid (MESH:C011459), ascorbic acid (MESH:D001205), Cu (MESH:D003300), manganese oxide (MESH:C027424), nervonic acid (MESH:C013147), ethanol (MESH:D000431), C18:3n3 (MESH:D017962), carotenoid (MESH:D002338), n6 fatty acids (MESH:D043371), zinc oxide (MESH:D015034), EPA (MESH:D015118), Retinoids (MESH:D012176), 2-thiobarbituric acid (MESH:C029684), heptadecanoic acid (MESH:C013102), Retinol (MESH:D014801), BHT (MESH:D002084), phenolic acids (MESH:C017616), Fe (MESH:D007501), arachidonic acid (MESH:D016718), C18:0 (MESH:C031183), MDA (MESH:D015104), Vitamin E (MESH:D014810), dichloromethane (MESH:D008752), n3 PUFA (MESH:D015525), ferrous sulfate (MESH:C020748), acetonitrile (MESH:C032159)
- **Species:** Gallus gallus (bantam, species) [taxon 9031], Homo sapiens (human, species) [taxon 9606], Saccharomyces cerevisiae (baker's yeast, species) [taxon 4932]

## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC12940071/full.md

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Source: https://tomesphere.com/paper/PMC12940071