# Anti-Inflammatory Effects on Periodontal Tissue and Antibacterial Effects on Oral Bacteria of Chlorogenic Acid

## TL;DR

This study shows that chlorogenic acid from coffee beans can reduce inflammation in periodontal tissues and inhibit harmful oral bacteria, suggesting its potential in preventing gum disease.

## Contribution

The novel contribution is demonstrating the anti-inflammatory and antibacterial efficacy of chlorogenic acid in periodontal disease prevention.

## Key findings

- Chlorogenic acid reduced inflammatory cytokine expression in human gingival and periodontal ligament fibroblasts.
- Chlorogenic acid inhibited the proliferation of key oral bacteria like Streptococcus mutans and Porphyromonas gingivalis.

## Abstract

Objectives: Combining mechanical plaque control, the physical removal of oral biofilm, with chemical plaque control, the use of agents to inhibit biofilm formation, is effective in preventing periodontal disease. Chlorogenic acid (CGA) found in coffee beans has medicinal effects, such as anti-inflammatory and antibacterial properties. Periodontal pathogens are difficult to reach in certain areas with traditional self-care tools, such as toothbrushes. Additionally, the viscous biofilm is difficult to remove using mechanical plaque control alone. Therefore, this study aimed to evaluate the efficacy of CGA in chemical plaque control. Methods: The mRNA and protein expression of inflammatory cytokines in lipopolysaccharide (LPS)-stimulated human gingival fibroblasts (HGFs) and human periodontal ligament fibroblasts (HPDLs) in the presence of CGA were analyzed using reverse transcription-qPCR and enzyme-linked immunosorbent assay. Additionally, the proliferation levels of oral bacteria in the presence of CGA were evaluated. Results: CGA suppressed mRNA and protein expression levels of the inflammatory cytokines, interleukin (IL)-1β and IL-8, in HGFs and HPDLs stimulated with Porphyromonas gingivalis LPS. Furthermore, CGA inhibited bacterial proliferation of Streptococcus mutans, Aggregatibacter actinomycetemcomitans, P. gingivalis, and Fusobacterium nucleatum. Conclusions: This study demonstrated that CGA exhibits anti-inflammatory effects on gingiva and periodontal ligaments, and antibacterial effects against oral bacteria. These results indicate the potential application of CGA in chemical plaque control and suggest its use in preventing periodontal disease progression.