# A Trichoderma hamatum Biostimulant Modulates Physiology and Gene Expression to Enhance Lettuce Salt Tolerance

## TL;DR

A biostimulant from Trichoderma hamatum helps lettuce plants tolerate salt stress by improving growth and reducing damage.

## Contribution

This study demonstrates a new biostimulant's ability to enhance salt tolerance in lettuce through physiological and genetic mechanisms.

## Key findings

- YF application significantly improved plant growth under salt stress.
- YF reduced oxidative damage and promoted proline accumulation.
- YF up-regulated key stress-responsive genes like NCED1, NCED2, WRKY58, and P5CSs.

## Abstract

Soil salinity is a major constraint on global agricultural productivity. This study evaluated the efficacy of a cell-free extract from Trichoderma hamatum (designated BEYF) in enhancing salt stress tolerance in lettuce (Lactuca sativa). Lettuce plants under normal and salt-stressed conditions exposed to 200 mM NaCl were treated with either water or YF (the working solution of BEYF) at concentrations of 0.05, 0.10, and 0.25 mg/L. Compared to the control, YF application significantly improved plant growth under salt stress, as indicated by increased plant height, biomass, leaf area, and other agronomic traits. Physiologically, YF mitigated oxidative membrane damage, as indicated by reduced electrolyte leakage and malondialdehyde (MDA) content, while promoting the accumulation of the osmoprotectant proline. Histochemical staining further confirmed that YF effectively suppressed hydrogen peroxide (H2O2) accumulation and preserved cell viability under salt stress. At the molecular level, YF significantly up-regulated the expression of key stress-responsive genes, including those involved in abscisic acid biosynthesis (NCED1, NCED2), signaling (WRKY58), and proline synthesis (P5CSs). Collectively, our findings demonstrate that BEYF enhances lettuce salt tolerance through integrated physiological, cellular, and transcriptional adaptations, supporting its potential as a sustainable biostimulant for improving crop cultivation in saline soils.