Integration of metabolomics and transcriptomics to reveal metabolic characteristics and the role of mTORC1 in β-cell proliferation induced by a short-term high-fat diet
Jiajia Wang, Jing Li, Yunshan Li, Mengran Liu, Shan Huang, Wenyi Li
TL;DR
This study shows that a short-term high-fat diet promotes pancreatic β-cell growth through the mTORC1 pathway, which can be blocked by rapamycin.
Contribution
The study identifies mTORC1 as a key driver of β-cell proliferation in response to a high-fat diet.
Findings
A short-term high-fat diet increases pancreatic β-cell proliferation without affecting insulin sensitivity.
The mTORC1 signaling pathway is crucial for diet-induced β-cell proliferation.
Rapamycin inhibits β-cell proliferation caused by the high-fat diet.
Abstract
Pancreatic β-cell proliferation is essential for maintaining the balance of β-cell mass, and an elevated metabolic load can stimulate their proliferation. Numerous studies have shown that a short-term high-fat diet increases metabolic load without affecting insulin sensitivity, thereby promoting the proliferation of pancreatic β-cells. However, the underlying mechanisms of this effect remain to be fully elucidated. A model has been constructed in our study to emulate pancreatic β-cell proliferation induced by a short-term high-fat diet, aiming to scrutinize the underlying mechanisms. Integrated transcriptomic and metabolomic analyses suggest that the mTORC1 signaling pathway may be crucial in this induced proliferation. Further analysis revealed that rapamycin, a specific inhibitor of the mTORC1 pathway, can inhibit proliferation induced by the short-term high-fat diet. Our study…
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Taxonomy
TopicsPancreatic function and diabetes · PI3K/AKT/mTOR signaling in cancer · FOXO transcription factor regulation