Network Pharmacology-Based Analysis Reveals the Mechanisms of the Tibetan Medicinal Plant Meconopsis quintuplinervia Against COPD and NAFLD: Insights from LC-MS/MS Profiling and Antioxidant/Anti-Inflammatory Activities

TL;DR

This study explores how a Tibetan medicinal plant helps treat lung and liver diseases by analyzing its chemical makeup and biological effects.

Contribution

The study identifies specific flavonoids in Meconopsis quintuplinervia and their mechanisms against COPD and NAFLD using network pharmacology.

Findings

MQ extract showed strong antioxidant and anti-inflammatory effects in cellular assays.

LC-MS/MS identified taxifolin as the main compound in the extract.

Flavonoids in the extract target pathways like NF-κB and SREBP-1 to reduce inflammation and lipid accumulation.

Abstract

Meconopsis quintuplinervia is traditionally used in Tibetan medicine for diseases of the lung and liver. This study investigated the antioxidant and anti-inflammatory activities of its extract (MQ extract), analyzed its chemical composition, and explored the potential therapeutic mechanisms against chronic obstructive pulmonary disease (COPD) and non-alcoholic fatty liver disease (NAFLD) using network pharmacology. MQ extract demonstrated effective scavenging of DPPH and ABTS radicals, with activity comparable to ascorbic acid and Trolox. In cellular assays, the extract dose-dependently reduced ROS levels in H2O2-induced B16-F10 and RAW264.7 cells and significantly inhibited NO production in LPS-stimulated RAW264.7 macrophages. Quantitative analysis showed total phenolic content of 90.54 ± 0.91 mg/g and total flavonoid content of 44.48 ± 0.43 mg/g. LC-MS/MS analysis identified taxifolin as the predominant constituent at approximately 2.39%. Network pharmacology and molecular docking studies revealed that flavonoids including catechin, isorhamnetin, kaempferol, luteolin, naringenin, nobiletin, quercetin, and taxifolin interacted with therapeutic targets for COPD and NAFLD. These compounds likely exerted effects by inhibiting NF-κB signaling, downregulating pro-inflammatory cytokines (TNF-α, IL-6, IL-1β), and enhancing antioxidant enzyme activities (SOD), while also reducing hepatic lipid accumulation through SREBP-1 suppression. Our findings elucidated why Tibetan medicine traditionally uses M. quintuplinervia to treat pulmonary and hepatic disorders.

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