Development of a 3D Skin Model for Studying Melanoma Progression
Dragana P. C. de Barros, Sara Ventura, Madalena Duque, Vanessa Ribeiro, Ana Sofia Lopes, Rita Zilhão, Ana Rita Carlos, Abel Oliva
TL;DR
A 3D skin model was developed to study melanoma progression and drug responses, offering a more realistic alternative to animal testing.
Contribution
The development of a 3D melanoma skin model that mimics tumor progression and immune evasion traits.
Findings
The 3D model supports melanoma cell invasion and recapitulates key features like ECM remodeling and EMT.
The model exhibits cytokine profiles similar to those seen in melanoma inflammation and immune evasion.
The model can be used for personalized medicine strategies using patient-derived cells.
Abstract
Despite advances in the treatment of cutaneous melanoma, there is still a high percentage of patients who fail to respond or develop resistance to treatment. Establishing robust in vitro melanoma models will enable mechanism-based drug screening while reducing animal testing. In this work, a three-dimensional (3D) melanoma skin model (3DMSM) was developed on a porous scaffold. The culture of three melanoma cell lines (SKMEL-1, A375, and G361) in co-culture with human fibroblasts, melanocytes, and keratinocytes allowed the formation of the dermis, and stratified epidermis. Tumors were established in this model using two methodologies: adding previously formed melanoma cell aggregates (CA) or seeding melanoma cells directly into the dermis (CD). In this model, melanoma cells remain in their original microenvironment and, after proliferation, invade the basal layer. The model recapitulates…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13
Figure 14
Figure 15Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
Topics3D Printing in Biomedical Research · Mathematical Biology Tumor Growth · Cutaneous Melanoma Detection and Management