# FoF1-ATPase-Mediated Proton Homeostasis Is the Dominant Mechanism Underlying Post-Acidification of Streptococcus thermophilus

## TL;DR

This study identifies how proton homeostasis via FoF1-ATPase helps Streptococcus thermophilus strains resist post-acidification in yogurt.

## Contribution

The study reveals FoF1-ATPase-mediated proton homeostasis as the key mechanism behind strain-specific post-acidification in S. thermophilus.

## Key findings

- FS strain accumulates more lactic acid and maintains intracellular pH better than FW under acid–cold stress.
- FS shows higher FoF1-ATPase activity and ATP levels, indicating better proton homeostasis.
- Transcriptomics show FS uses integrated regulatory pathways to sustain proton stability and reduce post-acidification.

## Abstract

Excessive post-acidification remains a major quality concern in yogurt production, yet the strain-specific mechanisms in Streptococcus thermophilus starter cultures are unclear. This study compared the post-acidification capacity of FS (strong) and FW (weak) strains through integrated physiological and molecular analyses to elucidate the dominant role of FoF1-ATPase–mediated proton homeostasis during storage. Although both strains exhibited similar acidification and lactose consumption during fermentation, FS accumulated more lactic acid during storage (6.89 vs. 6.49 g/L) and showed a smaller decrease in intracellular pH (ΔpHi 0.08 vs. 0.26), indicating superior proton homeostasis under acid–cold stress. Physiological assays revealed that FS showed higher FoF1-ATPase activity (1.17 μmol Pi/min/mg protein) and ATP levels (0.39 μmol/mg protein) at the storage endpoint. FS also maintained a membrane with a lower UFA/SFA ratio of 1.90, suggesting increased rigidity. Transcriptomics further showed that FS reinforced the FoF1-ATPase efflux pathway, aided by auxiliary neutralization and membrane-stress pathways. FS suppressed energy-costly biosynthesis and transport, forming a more integrated regulatory program than FW to sustain proton homeostasis. Notably, CcpA was upregulated in FS and was associated with this energy-conserving transcriptional profile, which may support proton transport and contribute to improved proton stability and reduced post-acidification under acid–cold stress. These findings provide mechanistic insights into strain-specific post-acidification and offer molecular targets for starter culture selection.