# Oak (Quercus petraea) Leaf-Based Kombucha: A Sustainable Approach to Fermented Beverages

**Authors:** Tomas Pencak, Dani Dordevic, Dominika Kotianova, Fouad Ali Abdullah Abdullah, Galia Zamaratskaia

PMC · DOI: 10.3390/foods15040707 · Foods · 2026-02-14

## TL;DR

This paper explores using oak leaves as a sustainable alternative to black tea for making kombucha, finding similar health benefits.

## Contribution

The study introduces oak leaves as a novel, sustainable substrate for kombucha fermentation.

## Key findings

- Fermentation of oak leaves increased phenolic content and antioxidant capacity.
- Beverages from oak leaves had comparable characteristics to black tea kombucha.
- Oak leaf kombucha showed effective acidification with lowered pH.

## Abstract

Kombucha is traditionally produced by fermenting black tea (Camellia sinensis), but exploring alternative plant materials could enhance sustainability and resource diversity. The study evaluated the potential of oak (Quercus petraea) leaves as a substrate for kombucha fermentation. Fresh and processed oak leaves were used to prepare kombucha infusions fermented for different time periods and compared with black tea kombucha, as well as unfermented infusions. Fermentation increased total phenolic content and antioxidant capacity in the dried and crushed leaf treatments, while lowering pH, which confirms effective acidification. Overall, beverages produced from dried and crushed oak leaves exhibited phenolic and antioxidant characteristics comparable to those of black tea kombucha. These findings suggest that Q. petraea leaves may represent a sustainable (due to the vast amount of raw material), same as not traditional alternative for kombucha production, contributing to the diversification of fermentation substrates in functional beverage development.

## Linked entities

- **Species:** Quercus petraea (taxon 38865), Camellia sinensis (taxon 4442)

## Full-text entities

- **Diseases:** Infusions (MESH:D000075662), inflammatory (MESH:D007249), injury to (MESH:D014947)
- **Chemicals:** Na2CO3 (MESH:C005686), sucrose (MESH:D013395), polyphenol (MESH:D059808), TPTZ (MESH:C002849), flavonoids (MESH:D005419), anthocyanins (MESH:D000872), acetate (MESH:D000085), Ceylon black tea (-), tannins (MESH:D013634), phenolic acids (MESH:C017616), water (MESH:D014867), ethanol (MESH:D000431), HCl (MESH:D006851), acetic acid (MESH:D019342), gallic acid (MESH:D005707), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (MESH:C010643), DPPH (MESH:C004931)
- **Species:** Oryza sativa (Asian cultivated rice, species) [taxon 4530], Camellia sinensis (black tea, species) [taxon 4442], Quercus robur (English oak, species) [taxon 38942], Quercus resinosa (species) [taxon 2004451], Stevia (genus) [taxon 55669], Quercus pyrenaica (species) [taxon 453298], Homo sapiens (human, species) [taxon 9606], Acetobacter pasteurianus (species) [taxon 438], Quercus petraea (durmast oak, species) [taxon 38865], Zygosaccharomyces bailii (species) [taxon 4954], Saccharomyces cerevisiae (baker's yeast, species) [taxon 4932], Komagataeibacter xylinus (species) [taxon 28448]

## Full text

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## References

48 references — full list in the complete paper: https://tomesphere.com/paper/PMC12939272/full.md

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Source: https://tomesphere.com/paper/PMC12939272