# Assessment of Salivary Parameters—pH, Buffering Capacity and Flow-Associated with Caries Susceptibility

**Authors:** Alexandru Ștefârță, Mihaela Roxana Brătoiu, Maria Alexandra Rădoi, Veronica Mercuț, Mihaela Ionescu, Monica Scrieciu, Ileana-Cristiana Petcu, Petre-Costin Mărășescu, Marina Olimpia Amărăscu, Adrian Marcel Popescu, Diana-Elena Vlăduțu

PMC · DOI: 10.3390/diagnostics16040625 · Diagnostics · 2026-02-20

## TL;DR

This study shows that low saliva pH and poor buffering capacity are linked to higher dental caries risk in young adults.

## Contribution

The study establishes a correlation between salivary parameters and caries susceptibility in a young adult population.

## Key findings

- Low salivary pH was significantly associated with higher DMFT values.
- Participants with low buffering capacity had higher DMFT values compared to those with normal capacity.

## Abstract

Background/Objectives: Saliva plays an essential role in maintaining the oral ecological balance, and its quantitative and qualitative characteristics may influence susceptibility to dental caries. The aim of this study was to determine susceptibility to dental caries based on the DMFT index and to establish a correlation between caries experience and salivary parameters in a group of young adults. Methods: This cross-sectional study was conducted between July and November 2025 on a sample of 87 fourth-year students from the Faculty of Dentistry in Craiova. Each participant underwent an intraoral clinical examination to determine the DMFT index. The salivary parameters assessed included unstimulated salivary flow rate, saliva consistency, salivary pH, stimulated salivary flow rate, and buffering capacity, using the GC Saliva-Check Buffer kit. Statistical analyses were performed using SPSS (Statistical Package for Social Sciences) software, version 26 (SPSS Inc., Armonk, NY, USA). Results: The mean DMFT index value for the entire sample was 8.26 ± 4.481, with higher values observed among female participants. Low salivary pH was significantly associated with higher DMFT values. Participants with low or very low buffering capacity exhibited higher DMFT values compared to those with normal capacity, indicating that a reduced ability to neutralize salivary acidity is associated with increased caries activity. Conclusions: The results indicate that salivary pH and buffering capacity are important factors in dental caries susceptibility among young adults. The integration of salivary testing into the diagnostic assessment of caries risk may contribute to personalized and effective preventive strategies.

## Linked entities

- **Diseases:** dental caries (MONDO:0005276)

## Full-text entities

- **Genes:** LPO (lactoperoxidase) [NCBI Gene 4025] {aka SPO}, LYZ (lysozyme) [NCBI Gene 4069] {aka AMYLD5, LYZF1, LZM}, SLC17A5 (solute carrier family 17 member 5) [NCBI Gene 26503] {aka AST, ISSD, NSD, SD, SIALIN, SIASD}
- **Diseases:** irritation (MESH:D001523), diabetes (MESH:D003920), injury to (MESH:D014947), foodborne microbial disease (MESH:D005517), oral diseases (MESH:D009059), COPD (MESH:D029424), tooth eruption (MESH:D014079), cardiovascular diseases (MESH:D002318), dental diseases (MESH:D009057), Caries (MESH:D003731), Oral Disorders (MESH:D009056), fungal infections (MESH:D009181), xerostomia (MESH:D014987), dentition damage (MESH:C566644), systemic (MESH:D015619)
- **Chemicals:** histidine (MESH:D006639), wax (MESH:D014885), phosphate (MESH:D010710), sugars (MESH:D000073893), ammonia (MESH:D000641), chloride (MESH:D002712), water (MESH:D014867), aspirin (MESH:D001241), fluoride (MESH:D005459), urea (MESH:D014508), hydroxyapatite (MESH:D017886), arginine (MESH:D001120), Bicarbonate (MESH:D001639), carbohydrate (MESH:D002241), maltose (MESH:D008320), sodium (MESH:D012964), lysine (MESH:D008239), hydrogen (MESH:D006859), glucose (MESH:D005947), calcium (MESH:D002118), fructose (MESH:D005632), CO2 (MESH:D002245), sucrose (MESH:D013395)
- **Species:** Homo sapiens (human, species) [taxon 9606]

## Full text

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## Figures

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## References

63 references — full list in the complete paper: https://tomesphere.com/paper/PMC12939177/full.md

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Source: https://tomesphere.com/paper/PMC12939177