# Modulation of Corticotropin-Releasing Hormone Receptor Expression During In Vitro Keratinocyte Differentiation

## TL;DR

This paper explores how stress-related hormone receptors change during skin cell development, finding that different receptors dominate at different stages.

## Contribution

The study provides new insights into the differential expression of CRHR1 and CRHR2 in keratinocytes during in vitro differentiation.

## Key findings

- CRHR1 is more abundant in early stages of keratinocyte differentiation.
- CRHR2 becomes more prominent at advanced stages of differentiation.
- The study highlights a potential link between stress response and skin cell development.

## Abstract

Corticotropin-releasing hormone (CRH) and its receptors CRHR1 and CRHR2 are major actors in the stress response and are well established as components of the hypothalamic–pituitary–adrenal (HPA) axis. Evidence also suggests they are expressed in peripheral tissues and, more interestingly, in the skin. While CRHR1 expression in keratinocytes is documented in terms of presence or absence, data on CRHR2 remain sparse. Moreover, there is no detailed description of the exact localization of CRHR1/2 receptors within the different layers of the epidermis, leaving this question fully unexplored. To better understand the link between stress and skin disorders, we aimed to investigate the differential expression of CRHR1 and CRHR2 in keratinocytes, depending on their level of differentiation. In vitro results demonstrated that CRHR1 appears to be more abundant at early stages of differentiation and CRHR2 at more advanced stages.