# Design Features of a Titanium Mesh for Guided Bone Regeneration and In Vivo Testing in Vitamin D3 Deficiency Condition

## TL;DR

A titanium mesh was designed and tested in vitamin D3-deficient rabbits to support bone regeneration, showing promising tissue response and biocompatibility.

## Contribution

A novel titanium mesh design was developed and tested in a vitamin D3-deficient model for guided bone regeneration.

## Key findings

- The titanium mesh showed effective vascularization and osteogenesis in vitamin D3-deficient rabbits.
- Histological analysis revealed dense connective tissue and active osteogenesis three months post-implantation.
- The mesh demonstrated acceptable biocompatibility and potential osteoconductive properties.

## Abstract

Prolonged tooth loss causes alveolar ridge atrophy, complicating implantation, especially in patients with impaired mineral metabolism. This study aimed to develop a personalized titanium mesh for guided bone regeneration and qualitatively evaluate its local tissue response in a vitamin D3-deficient rabbit model. A titanium mesh design has been developed in the form of a plate-shaped profile frame of a truncated pyramid with a solid upper base and perforated side faces. For testing in a rabbit model with vitamin D3 deficiency, a bone defect was created and repaired in the mandible using hydroxyapatite, an individual titanium mesh and a collagen membrane. Histological analysis was performed in the Laboratory of Digital Microscopic Analysis. The optimized geometry and parameters of the mesh openings contributed to effective vascularization and osteogenesis. In the postoperative period (3, 5 and 7 days), moderate edema and hyperemia were noted with their complete leveling by the 7th day (p < 0.05). According to the histological examination, 3 months after the installation of the titanium mesh, the formation of dense connective tissue with signs of active osteogenesis was observed in the defect area, including zones of mineralized bone trabeculae, osteocytes and osteon elements. The findings of this study indicate acceptable biocompatibility of the developed titanium structure and suggest osteoconductive potential, which, however, needs to be confirmed in controlled, quantitatively powered studies.