# A Comprehensive Review of Biochemical Insights and Advanced Packaging Technologies for Shelf-Life Enhancement of Temperate Fruits

## TL;DR

This review explores how biochemical changes in temperate fruits after harvest affect their shelf life and how advanced packaging technologies can help reduce spoilage and losses.

## Contribution

The paper provides a comprehensive review of biochemical insights and packaging technologies for extending the shelf life of temperate fruits.

## Key findings

- Post-harvest biochemical changes like ethylene production and enzymatic activity significantly impact fruit spoilage.
- Advanced packaging technologies, such as biodegradable films and smart packaging, show promise in extending shelf life.
- Each packaging technology has specific limitations that need to be addressed for optimal use.

## Abstract

Temperate fruits, mostly comprising pome, stone fruits, and berries with immense nutritional benefits and a storehouse of various therapeutic phytochemicals, are prone to several physiological disorders immediately after harvest. The etiology, symptom progression, and decay incidence are influenced by pre-harvest and post-harvest factors, causing significant economic loss with respect to both the energy and economics invested. Respiratory end products, ethylene generation, and enzymatic activities interact to influence the metabolic response and associated biochemical variation. Advanced packaging technologies have emerged as innovative solutions to curtail these post-harvest problems. The design and development of novel packaging technologies need to critically understand the respiratory behavior of the fruits and their associated metabolic functions. A desirable polymer or packaging technology should exhibit enhanced barriers to the gases while providing adequate support to the fruit matrix. In addition, it should also fulfill the role of environmental sustainability and the circular economy. The outcome of this review will highlight the importance of proper post-harvest procedure, appropriate pretreatment, packaging matrix selection, and the storage conditions for effective and enhanced shelf-life storage. Therefore, this review was structured in two phases; the first phase discusses the biochemical understanding of the fruit during storage and transit in response to stress factors. The next phase highlights the various packaging interventions (polymers, biodegradable films, edible coatings, smart packaging, nano-packaging) taken to address these issues, with a key focus on shelf-life enhancement. Further, the key limitations of each technology are appraised.