# Clinical Outcomes of ICSI Using Advanced Quantitative Phase Microscopy

## TL;DR

This study shows that using advanced 3D sperm analysis with QPM improves pregnancy and delivery rates in ICSI compared to traditional methods.

## Contribution

The study introduces QPM as a novel, non-invasive sperm selection method for ICSI with improved clinical outcomes.

## Key findings

- QPM group had higher pregnancy rates (56.2%) compared to standard ICSI (18.1%) and IMSI (16.6%).
- Delivery rates were significantly higher in the QPM group (50%) than in standard ICSI (9%) and IMSI (16.6%).
- No pregnancy complications were recorded in the QPM group, indicating its safety.

## Abstract

This study evaluated the clinical outcomes associated with quantitative phase microscopy (QPM), an advanced 3D morphological sperm analysis technique using a virtual, stained model developed in our facility that indicates whether the inspected sperm should be selected for intracytoplasmic sperm injection (ICSI). We prospectively compared the clinical outcomes of three groups of cycles: (1) one with the QPM assessment, (2) one with conventional ICSI, without using QPM in the same patient, and (3) one with conventional ICSI cycles of an historical cohort of patients who did not undergo QPM. The outcomes of cycles using QPM vs. intracytoplasmic morphologically selected sperm injection (IMSI) cycles were also compared. A total of 1243 cycles were included. Pregnancy and delivery rates were higher among the QPM group compared to the standard ICSI group (56.2% vs. 18.1%, p = 0.04 and 50% vs. 9%, respectively, p = 0.02). Pregnancy and delivery rates were also higher among the QPM group compared to the IMSI group (56.2% vs. 16.6%, p = 0.01 and 50% vs. 16.6%, respectively, p = 0.03). No pregnancy complications were recorded in the QPM group. We conclude that QPM is a safe, non-invasive sperm selection technique for ICSI, with the potential to improve clinical pregnancy and delivery rates for couples with male infertility.