# Comparative Study of B Vitamins in Multiple Tissues of Oilseed Crops and Leafty Vegetables Reveal Sesame as a Valuable Resource in Vitamin B3, B6 and B12

**Authors:** Yijia Zhang, Ting Zhou, Zishu Luo, Desawi Hdru Teklu, Lei Wang, Rong Zhou, Wei Wang, Jun You, Huan Li, Linhai Wang

PMC · DOI: 10.3390/antiox15020224 · Antioxidants · 2026-02-09

## TL;DR

This study compares B vitamin levels in oilseed crops and leafy vegetables, finding that sesame is a rich source of vitamins B3, B6, and B12.

## Contribution

The study identifies sesame as a novel, plant-based source of multiple B vitamins with high antioxidant potential.

## Key findings

- Sesame seeds have 1.6–8.2-fold higher B3 levels than other oilseeds.
- Sesame leaves contain 2.57–8.31-fold more B6 than spinach and lettuce.
- Sesame leaves show ~13–20 times higher B12 levels than other leaf samples.

## Abstract

B vitamins are essential micronutrients for human health with prominent antioxidant properties, capable of scavenging reactive oxygen species (ROS) and maintaining redox homeostasis, protecting cells from oxidative damage. To address global nutrient deficiencies and identify plant-based antioxidant sources, this study quantified seven B vitamins (B1, B2, B3, B5, B6, B9, B12) in seeds, leaves, and seedlings of five oilseeds (sesame, peanut, soybean, rapeseed, perilla) and two leafy vegetables (spinach, lettuce) via LC-MS/MS, revealing distinct species- and tissue-specific patterns. Notably, sesame seeds exhibited exceptional vitamin B3 (niacin, 39.3 μg/g), surpassing other oilseeds1.6–8.2-fold; its leaves contained outstanding vitamin B6 (2.88 μg/g), 2.57–8.31-fold higher than spinach (1.12 μg/g) and lettuce (0.34 μg/g), and vitamin B12 (0.44 μg/g) levels ~13–20 times higher than other leaf samples. Sesame seedlings recorded high vitamin B6 (1.6 μg/g) and B12 (0.1 μg/g) among the oilseed crops seedlings. These findings highlight sesame as a multifunctional B vitamin resource for antioxidant nutrition, supporting dietary optimization, crop biofortification, and mitigation of global B vitamin inadequacies via plant-based solutions.

## Linked entities

- **Chemicals:** B1 (PubChem CID 5352030), B2 (PubChem CID 6328200), B3 (PubChem CID 3035014), B5 (PubChem CID 23688952), B9 (PubChem CID 8830), B12 (PubChem CID 54605677), niacin (PubChem CID 938)
- **Species:** Perilla (taxon 48385)

## Full-text entities

- **Genes:** SOD [NCBI Gene 105166989]
- **Diseases:** neural tube defects (MESH:D009436), neurological disorders (MESH:D009461), vitamin B12 deficiency (MESH:D014806), beriberi disease (MESH:D001602), pellagra (MESH:D010383), B vitamin deficiencies (MESH:D014804), injury to (MESH:D014947), neurological impairments (MESH:D009422), chronic (MESH:D002908), megaloblastic anemia (MESH:D000749), vitamin B1 deficiency (MESH:C566196), vitamin B9 (MESH:D014802), cardiovascular disease (MESH:D002318), B3 deficiency disorder (OMIM:120050), micronutrient deficiencies (MESH:D007153), dietary (MESH:D000740)
- **Chemicals:** hydroxyl radicals (MESH:D017665), sodium hydroxide (MESH:D012972), B3 (MESH:C053396), biotin (MESH:D001710), hydrochloric acid (MESH:D006851), B12 (MESH:C034730), water (MESH:D014867), Vitamin B3 (MESH:D009536), chlorophyll (MESH:D002734), acetonitrile (MESH:C032159), nitrogen (MESH:D009584), formic acid (MESH:C030544), PTFE (MESH:D011138), pyridoxine (MESH:D011736), oxygen (MESH:D010100), phytates (MESH:D010833), methanol (MESH:D000432), folate (MESH:D005492), ROS (MESH:D017382), NAD+ (MESH:D009243), Vitamin B6 (MESH:D025101), acetaldehyde (MESH:D000079), B9 (MESH:C014499), lipid (MESH:D008055), Vitamin B12 (MESH:D014805), pantothenic acid (MESH:D010205), B2 (MESH:C023970), FMN (MESH:D005486), GSH (MESH:D005978), Vitamin B2 (MESH:D012256), oil (MESH:D009821), niacin (MESH:D009525), homocysteine (MESH:D006710), amino acids (MESH:D000596), thiamine (MESH:D013831), NADP+ (MESH:D009249), betaine (MESH:D001622), H2O2 (MESH:D006861), B1, B3, B5, B6, B12 (-)
- **Species:** Stenotrophomonas sp. O (species) [taxon 1355440], Arachis hypogaea (goober, species) [taxon 3818], Pseudomonas aeruginosa (species) [taxon 287], Glycine max (soybean, species) [taxon 3847], Spinacia oleracea (spinach, species) [taxon 3562], Sesamum indicum (beniseed, species) [taxon 4182], Paracoccus sp. L (species) [taxon 166788], Oryza sativa (Asian cultivated rice, species) [taxon 4530], Perilla (genus) [taxon 1313341], Homo sapiens (human, species) [taxon 9606], Manihot esculenta (cassava, species) [taxon 3983], Lentinula edodes (shiitake mushroom, species) [taxon 5353]

## Full text

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## Figures

6 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12938314/full.md

## References

42 references — full list in the complete paper: https://tomesphere.com/paper/PMC12938314/full.md

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Source: https://tomesphere.com/paper/PMC12938314