# Genetic Association Analysis of Skin Traits and the TAF11 Gene in Shenxian Pigs

## TL;DR

This study found that a mutation in the TAF11 gene is linked to thicker skin in Shenxian pigs, especially in female pigs, which could help improve leather quality and breeding.

## Contribution

The study identifies a TAF11 gene mutation associated with increased skin thickness in Shenxian pigs, particularly in sows.

## Key findings

- Significant regional variation in skin thickness and collagen content was observed across nine anatomical sites in Shenxian pigs.
- The TAF11 g.35543837 C > G variant is significantly associated with increased skin thickness, especially in gilts.
- The CG genotype at the TAF11 locus results in greater skin thickness compared to the CC genotype at multiple sites.

## Abstract

The thickness and collagen content of pig skin are key determinants of leather quality. This study systematically measured skin traits across nine anatomical sites in Shenxian pigs, revealing significant regional variation in both thickness and collagen content. The results indicate that a specific mutation in the TAF11 gene is significantly associated with increased skin thickness, with this effect being more pronounced in sows. This finding suggests that this genetic locus holds potential as a molecular marker for assisting in the future selection of breeding stock with superior skin thickness. The study provides a genetic basis for developing the hide resource value of Shenxian pigs and contributes to enhancing the overall economic efficiency of their production.

This study aimed to characterize the site-specific variation in skin traits of Shenxian pigs and to identify key genetic loci regulating skin thickness. A total of 50 Shenxian pigs were selected, and skin samples were collected from nine different anatomical sites. Total skin thickness was precisely measured, and collagen content was determined for each site. Based on literature review and database screening, TAF11 was identified as a candidate gene. Genotyping of the g.35543837 locus was performed using Sanger sequencing and KASP, followed by association analysis between different genotypes and skin thickness traits. The results showed significant site-specific variations in skin thickness (1.26–7.20 mm) and collagen content (7.01–24.54 g/100 g) in Shenxian pigs. Association analysis revealed that the TAF11 g.35543837 C > G variant was significantly associated with increased skin thickness, with the effect being particularly evident in gilt. Individuals with the CG genotype exhibited greater skin thickness at multiple anatomical sites compared with those carrying the CC genotype. This study preliminarily identified a potential locus associated with skin thickness in Shenxian pigs within the TAF11 gene. The sex-dependent effect observed at this locus provides a new clue for understanding the genetic basis of this complex trait and offers valuable information for the genetic improvement of skin-related traits in Shenxian pigs.