# Bioactivity and Regenerative Potential of Cannabidiol in Human Dental Pulp Stem Cells: A Scoping Review of In Vitro Studies

## TL;DR

This review explores how cannabidiol (CBD) affects human dental pulp stem cells in lab studies, suggesting it may help in dental tissue repair.

## Contribution

The study is the first scoping review to evaluate CBD's effects on osteogenic/odontogenic differentiation of human dental pulp stem cells.

## Key findings

- CBD at low concentrations improved hDPSC viability, proliferation, migration, and differentiation.
- CBD activated MAPK and WNT/β-catenin pathways and increased odontogenic markers like DSPP, RUNX2, and osteocalcin.
- CBD shows potential for regenerative endodontics but requires further preclinical and clinical validation.

## Abstract

Cannabidiol (CBD), a nonpsychoactive compound derived from Cannabis sativa, has shown potential to influence cellular processes that are important for dental tissue repair. The aim of this scoping review was to map in vitro studies evaluating the influence of CBD on the osteogenic/odontogenic differentiation of human dental pulp stem cells (hDPSCs) in order to contribute to a better understanding of its therapeutic potential.

The review followed the Arksey and O′Malley framework, supported by the JBI Manual and PRISMA‐ScR guidelines. The protocol was registered on OSF (osf.io/zfhca/). Comprehensive searches were conducted from January to June 2025 in PubMed, EMBASE, BVS, Scopus, Web of Science, ScienceDirect, and SciELO. Only studies published in English were included.

Thirty articles were identified, and three in vitro studies met the eligibility criteria. At low concentrations (0.1–5 μM), CBD improved hDPSC viability, proliferation, migration, and differentiation. CBD also activated the mitogen‐activated protein kinase (MAPK) and wingless‐related integration site/beta‐catenin signaling (WNT/β‐catenin) pathways and increased the expression of odontogenic markers such as Sialophosphoprotein (DSPP), Runt‐related transcription Factor 2 (RUNX2), and osteocalcin.

CBD shows promise as a bioactive molecule in regenerative endodontics, supporting mineralization, regulating inflammatory mediators, and promoting critical cellular activities in hDPSCs. Nevertheless, the available evidence is limited and further preclinical and clinical studies are essential to develop therapeutic protocols and assess long‐term safety. These preliminary findings indicate CBD as a novel candidate for regenerative strategies in endodontics.