# The use of Diathermic Syncope® for stunning cattle

**Authors:** Søren Saxmose Nielsen, Julio Alvarez, Anette Boklund, Sabine Dippel, Fernanda Dorea, Jordi Figuerola, Mette S. Herskin, Miguel Angel Miranda Chueca, Eleonora Nannoni, Romolo Nonno, Anja B. Riber, Karl Stahl, Jan Arend Stegeman, Hans‐Hermann Thulke, Frank Tuyttens, Virginie Michel, Christoph Winckler, Mohan Raj, Antonio Velarde, Alexei Vyssotski, Yves Van der Stede, Aikaterini Manakidou

PMC · DOI: 10.2903/j.efsa.2026.9934 · EFSA Journal · 2026-02-26

## TL;DR

EFSA evaluated Diathermic Syncope® for stunning cattle and found it does not meet current animal welfare standards.

## Contribution

This paper provides a scientific assessment of a novel cattle stunning method against established welfare criteria.

## Key findings

- The data provided only partially fulfilled the necessary criteria for approval.
- DTS does not ensure a level of welfare equivalent to currently allowed methods.
- The assessment followed EFSA's guidance and included expert elicitation and literature review.

## Abstract

The EFSA Panel on Animal Health and Welfare (AHAW) was asked to deliver a scientific opinion on the use of Diathermic Syncope® (DTS) for stunning cattle. A dossier was provided by the applicant as the basis for an assessment of the extent to which the method is able to provide a level of animal welfare at least equivalent to that ensured by the currently allowed methods for stunning cattle. This scientific opinion followed the EFSA Guidance (2018) on the assessment criteria for applications for new or modified stunning methods regarding animal protection at time of killing. Under Council Regulation (EC) No 1099/2009, approval of novel stunning methods requires demonstration of (1) the absence of pain, distress or suffering until the onset of unconsciousness and (2) that the animal remains unconscious until death. An ad hoc Working Group (WG) by EFSA performed the assessment as follows: (1) check of provided data against the criteria laid down in the EFSA Guidance; (2) extensive literature search; (3) data extraction and quantitative assessment; (4) exercise based on non‐formal expert elicitation and qualitative assessment. Although the data and studies provided in the dossier only partially fulfilled the necessary criteria, they were sufficient to proceed with the animal welfare risk assessment. According to the data and the use of DTS parameterised by the applicant (delivering 160–200 kJ of energy and an incident power of 16–20 kW for 10 s), DTS does not ensure a level of welfare at least equivalent to one or more of the currently allowed methods listed in Annex I of Council Regulation (EC) No 1099/2009.

## Full-text entities

- **Genes:** ACHE (acetylcholinesterase (Yt blood group)) [NCBI Gene 540446], POMC (proopiomelanocortin) [NCBI Gene 281416], Ache (acetylcholinesterase) [NCBI Gene 83817]
- **Diseases:** tissue (MESH:D017695), Eyelid flutter (MESH:D005141), coma (MESH:D003128), tetanus (MESH:D013746), tonic (MESH:D004829), absence seizures (MESH:D004832), necrosis (MESH:D009336), Heat Stroke (MESH:D018883), absence of breathing (MESH:D012891), Epileptiform seizure (MESH:D014277), WC (MESH:C535499), loss of muscle tone (MESH:D009122), hyperventilation (MESH:D006985), absence of rhythmic breathing (MESH:D020922), skin tissue damage (MESH:D017437), pupillary dilation (MESH:D002311), TS (MESH:D005879), oedema (MESH:C536897), impaired immune function (MESH:D007154), death (MESH:D003643), brain (MESH:D001927), ataxia (MESH:D001259), consciousness (MESH:D003244), Apnoea (MESH:D001049), Grand mal epilepsy (MESH:D004830), brain damage (MESH:D001925), startle (MESH:D016750), muscle spasms (MESH:D013035), movement of the eyes (MESH:D015835), epidermal loss (MESH:D004814), epilepsy (MESH:D004827), ABMs (MESH:D000820), bradycardia (MESH:D001919), Skin burn (MESH:D002056), PUBLIC (MESH:C000719203), clonic seizure (MESH:D012640), LAPS (MESH:D007022), burn lesion (MESH:D002054), Hyperthermia (MESH:D005334), Distress (MESH:D012128), nystagmus (MESH:D009759), stroke (MESH:D020521), cerebral oedema (MESH:D001929), bleeding (MESH:D006470), tachycardia (MESH:D013610), lesions (MESH:D009059), hair loss (MESH:D000505), brain death (MESH:D001926), cardiac arrest (MESH:D006323), eye staring (MESH:C000719215), aneurysm (MESH:D000783), loss of consciousness (MESH:D014474), cancer (MESH:D009369), false aneurysm (MESH:D017541), Pain (MESH:D010146), skin loss (MESH:D012871), injuries (MESH:D014947), carotid occlusion (MESH:D016893), DTS (MESH:D013575), loss (MESH:D016388)
- **Chemicals:** quartz (MESH:D011791), glucose (MESH:D005947), formalin (MESH:D005557), CAS (MESH:D002118), ice (MESH:D007053), NADH (MESH:D009243), Eosin (MESH:D004801), ATP (MESH:D000255), CO2 (MESH:D002245), CP (MESH:D010725), aspartate (MESH:D001224), Catecholamines (MESH:D002395), Haematoxylin (MESH:D006416), aluminium (MESH:D000535), Captive bolt (-), H&amp;E (MESH:D006371), ethanol (MESH:D000431), noradrenaline (MESH:D009638), glutamate (MESH:D018698), glycogen (MESH:D006003), water (MESH:D014867), adrenaline (MESH:D004837), cyanoacrylate (MESH:D003487), N2 (MESH:D009584), cortisol (MESH:D006854), oxygen (MESH:D010100)
- **Species:** Bos indicus (Indicine cattle, species) [taxon 9915], Bos taurus (bovine, species) [taxon 9913], Cervus elaphus (red deer, species) [taxon 9860], Homo sapiens (human, species) [taxon 9606], Rattus norvegicus (brown rat, species) [taxon 10116], Sus scrofa (pig, species) [taxon 9823], Ovis aries (domestic sheep, species) [taxon 9940], Canis lupus familiaris (dog, subspecies) [taxon 9615], Mus musculus (house mouse, species) [taxon 10090]
- **Mutations:** C-12 C, C in 330, C-47 C, C-45 C, C in 800, C-40 C, C at 12, C-70 C, C at 14

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## Figures

18 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12936714/full.md

## References

122 references — full list in the complete paper: https://tomesphere.com/paper/PMC12936714/full.md

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Source: https://tomesphere.com/paper/PMC12936714