# Nocardia rubra cell wall skeleton-induced MARCO expression: implications for improved phagocytosis and cytokine secretion in tumor-associated macrophages

## TL;DR

This study shows that Nocardia rubra cell wall skeleton boosts immune function in tumor-associated macrophages by increasing MARCO expression, improving phagocytosis and cytokine production.

## Contribution

The study identifies MARCO as a novel mediator of Nr-CWS-induced macrophage reprogramming in HPV-related neoplasia.

## Key findings

- Nr-CWS upregulates MARCO expression in TAMs, enhancing phagocytic activity and proinflammatory cytokine secretion.
- MARCO expression is significantly altered in most tumor tissues and correlates with immune cell infiltration.
- MARCO inhibition suppresses Nr-CWS-induced immune functions in TAMs.

## Abstract

Nocardia rubra cell wall skeleton (Nr-CWS) demonstrates a significant therapeutic effect against human papillomavirus (HPV) infection, but its precise immunomodulatory mechanisms warrant further investigation. This study investigates how Nr-CWS influences tumor-associated macrophages (TAMs) immune functions through macrophage receptor with collagenous structure (MARCO)-mediated mechanisms.

Cervical tissues of three cervical intraepithelial neoplasia (CIN) patients receiving Nr-CWS monotherapy and HPV infection turning negative were collected before and after treatment, and gene microarray analysis was performed. MARCO expression and immune cell infiltration were further analyzed using transcriptomic data from 33 tumor types in The Cancer Genome Atlas (TCGA). In vitro, TAMs derived from Human Monocytic Leukemia Cell Line 1 (THP-1) cells were treated with Nr-CWS, and changes in MARCO expression, cytoskeletal rearrangement, pseudopod length, lysosome count, and cytokine secretion were assessed. MARCO inhibition experiments were also performed.

Gene microarray revealed significant upregulation of MARCO, a key phagosome pathway gene, post-treatment. TCGA analysis indicated that MARCO expression is significantly altered in most tumor tissues compared to normal tissues and is associated with the infiltration of multiple immune cell types, with a particularly strong correlation to macrophage abundance. Histologically, Nr-CWS increased MARCO⁺ macrophages in cervical tissues. In vitro, Nr-CWS elevated MARCO expression in TAMs, enhanced pseudopod formation, lysosome number, cytoskeleton reorganization, and promoted proinflammatory cytokine secretion. Conversely, MARCO inhibition suppressed these immune functions.

The study demonstrates that Nr-CWS enhances TAM anti-tumor immune function via MARCO upregulation, leading to improved phagocytic activity and proinflammatory response. These findings align with genomic and cellular evidence, suggesting MARCO as a key mediator in Nr-CWS-induced macrophage reprogramming, with implications for HPV-related neoplasia immunotherapy.