# Microbial biofortification of fermented foods: a review of probiotic-mediated nutrient enhancement

**Authors:** Fahad Saad Alhodieb

PMC · DOI: 10.3389/fnut.2026.1754233 · Frontiers in Nutrition · 2026-02-12

## TL;DR

This paper reviews how probiotic fermentation can boost nutrients in foods, helping combat deficiencies and improve gut and immune health.

## Contribution

The paper highlights novel uses of probiotics in enhancing nutrient availability and bioactive compounds in fermented foods.

## Key findings

- Probiotic fermentation increases vitamin and mineral levels in plant-based diets.
- Fermentation breaks down antinutrients and produces bioactive peptides and fatty acids.
- Biofortified foods improve gut health and immunity in lab, animal, and human studies.

## Abstract

Microbial biofortification via probiotic fermentation is a unique solution to reducing micronutrient deficiencies worldwide and it is a sustainable approach to prevention and German fermentation is widely applicable for plant-based diets as these micronutrients, such as B12 and K, are hardly present. Fermentative microbes such as Lactobacillus, Bifidobacterium, Propionibacterium synthesis of the vitamins like folate, riboflavin, vitamin K. They also facilitate the accessibility of minerals and increase the quality of proteins in many foods. This process not only enhances vitamins and minerals as a result of antinutrient such as phytate breakdown, but also bioactive peptides and short-chain fatty acids are produced. These are beneficial compounds for gut health and are helpful to the health of the immune system. Studies in labs, animals, and humans indicate that consumption of biofortified fermented foods increases micronutrient levels, promotes gut microbial balance, and increases immunity. In order to exploit this approach to its fullest potential, there are hurdles to overcome, ensuring that the strain remains viable, enhancing product taste, and overcoming regulatory hurdles. Future advances will require engineering strains of probiotics to produce even greater amounts of vitamins and implementing personalized microbiome information, as well as their public health interventions, in resource-limited situations.

## Linked entities

- **Chemicals:** B12 (PubChem CID 54605677), K (PubChem CID 813), phytate (PubChem CID 890)
- **Species:** Lactobacillus (taxon 1578), Bifidobacterium (taxon 1678), Propionibacterium (taxon 1743)

## Full-text entities

- **Genes:** DHFR (dihydrofolate reductase) [NCBI Gene 1719] {aka DHFR1, DYR}, mucin [NCBI Gene 100508689], GGCX (gamma-glutamyl carboxylase) [NCBI Gene 2677] {aka VKCFD1, VKGC}
- **Diseases:** pernicious anemia (MESH:D000752), inflammatory and metabolic disease (MESH:D008659), B12 deficiency (MESH:D014806), dysbiosis (MESH:D064806), sarcopenia (MESH:D055948), inflammation (MESH:D007249), chronic diseases (MESH:D002908), migraine (MESH:D008881), inflammatory bowel disease (MESH:D015212), deficiencies of the mineral type (MESH:C537337), osteoporosis (MESH:D010024), vitamin deficiencies (MESH:D014802), infections (MESH:D007239), Micronutrient deficiencies (MESH:D007153), malnutrition (MESH:D044342), anemia (MESH:D000740), calcification of arteries (MESH:D061205)
- **Chemicals:** erythrose-4-phosphate (MESH:C026959), B (MESH:D001895), ribulose-5-phosphate (MESH:C031524), vitamin C (MESH:D001205), acetic (MESH:D019342), inulin (MESH:D007444), glutamate (MESH:D018698), biogenic amines (MESH:D001679), GABA (MESH:D005680), branched chain amino acids (MESH:D000597), calcium phosphate (MESH:C020243), essential amino acid (MESH:D000601), propionic acid (MESH:C029658), isoprenoid (MESH:D013729), iron (MESH:D007501), phosphoenolpyruvate (MESH:D010728), dihydropteroate (MESH:C000978), valine (MESH:D014633), Leucine (MESH:D007930), water (MESH:D014867), B12 (MESH:C034730), crotonate (MESH:D003437), carbon (MESH:D002244), MK-7 (MESH:C062629), pentose phosphate (MESH:D010428), Vitamin K2 (MESH:D024482), histidine (MESH:D006639), vitamin E (MESH:D014810), nitrogen (MESH:D009584), isoleucine (MESH:D007532), polysaccharides (MESH:D011134), lactic acid (MESH:D019344), lactose (MESH:D007785), zinc (MESH:D015032), oxygen (MESH:D010100), ammonia (MESH:D000641), corrinoid (MESH:D045728), acids (MESH:D000143), sugars (MESH:D000073893), phosphorus (MESH:D010758), salt (MESH:D012492), phosphate (MESH:D010710), NaCl (MESH:D012965), metal (MESH:D008670), GTP (MESH:D006160), methionine (MESH:D008715), pyruvate (MESH:D019289), Phytate (MESH:D010833), succinate (MESH:D019802), threonine (MESH:D013912), anthranilate (MESH:C031385), SCFA (MESH:D005232), Calcium (MESH:D002118), saponin (MESH:D012503), Folate (MESH:D005492), pteridine (MESH:D011621), myo-inositol (MESH:D007294), magnesium (MESH:D008274), isochorismate (MESH:C052985), Tryptophan (MESH:D014364)
- **Species:** Carica papaya (mamon, species) [taxon 3649], Lens culinaris (lentil, species) [taxon 3864], Lactobacillus acidophilus (species) [taxon 1579], Vigna radiata (mung bean, species) [taxon 157791], Lactococcus lactis (species) [taxon 1358], Lactobacillus helveticus (species) [taxon 1587], Roseburia (genus) [taxon 841], Ruminococcus bromii (species) [taxon 40518], Amaranthus caudatus (amaranth, species) [taxon 3567], Brassica oleracea (wild cabbage, species) [taxon 3712], Saccharomyces cerevisiae (baker's yeast, species) [taxon 4932], Lactobacillus (genus) [taxon 1578], Aspergillus oryzae (species) [taxon 5062], Lacticaseibacillus rhamnosus (species) [taxon 47715], Leuconostoc mesenteroides (species) [taxon 1245], Mus musculus (house mouse, species) [taxon 10090], Propionibacterium freudenreichii (species) [taxon 1744], Escherichia coli (E. coli, species) [taxon 562], Cicer arietinum (chickpea, species) [taxon 3827], Heyndrickxia coagulans (species) [taxon 1398], Bacillus subtilis (species) [taxon 1423], Glycine max (soybean, species) [taxon 3847], Malus domestica (apple, species) [taxon 3750], Rhizopus microsporus var. oligosporus (varietas) [taxon 4847], Bifidobacterium longum (species) [taxon 216816], Faecalibacterium (genus) [taxon 216851], Lactiplantibacillus plantarum (species) [taxon 1590], Bifidobacterium breve (species) [taxon 1685], Limosilactobacillus reuteri (species) [taxon 1598], Streptococcus thermophilus (species) [taxon 1308], Propionibacterium (genus) [taxon 1743], Cucumis sativus (cucumber, species) [taxon 3659], Limosilactobacillus fermentum (species) [taxon 1613], Oryza sativa (Asian cultivated rice, species) [taxon 4530], Lactobacillus kefiranofaciens (species) [taxon 267818], Bifidobacterium bifidum (species) [taxon 1681], Bacillus subtilis subsp. natto (subspecies) [taxon 86029], Fascellina sp. A (species) [taxon 1373661], Levilactobacillus brevis (species) [taxon 1580], Bifidobacterium (genus) [taxon 1678], Homo sapiens (human, species) [taxon 9606], Lacticaseibacillus casei (species) [taxon 1582]

## Full text

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## Figures

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## References

120 references — full list in the complete paper: https://tomesphere.com/paper/PMC12935629/full.md

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Source: https://tomesphere.com/paper/PMC12935629