# Optimization of pesticide spray parameters and analysis of key physiological indicators for control efficiency against jujube leaf black spot

**Authors:** Rui-Xia Zhang, Jun-Ya Hao, Ke Xiao, Ping Liu

PMC · DOI: 10.3389/fpls.2026.1742081 · Frontiers in Plant Science · 2026-02-05

## TL;DR

This study finds the best pesticide spray settings and key plant indicators to control jujube leaf black spot disease efficiently and sustainably.

## Contribution

Identifies optimal spray parameters and key physiological indicators for graded control of jujube leaf black spot.

## Key findings

- Spray distance and angle significantly affect control efficiency based on disease severity levels.
- Soluble sugar content and enzyme activities (SOD, POD, PAL) correlate with control efficiency.
- SOD and POD are key indicators for evaluating the physiological impact of chemical control.

## Abstract

Jujube leaf black spot is a common foliar disease in jujube orchards. Precision spraying technology enhances pesticide utilization efficiency through accurate targeting and on-demand application, while simultaneously mitigating environmental risks.

In this study, 1,000 jujube black spot leaves with different disease severity levels were selected, labeled, and photographed. Chemical treatments were applied at varying spraying distances and angles according to the disease severity, with a water control group established for comparison. The control efficiency of spraying parameters on jujube leaf black spot across different disease severity levels and their physiological effects on the leaves were measured.

The spraying parameters significantly influenced the control efficiency across different disease severity levels. For level 0 (prevention), a distance of 0.9 m and an angle of 0°; for level 1, a distance of 1.2 m and an angle of 0°; for level 2, a distance of 0.6 m and an angle of 40°; for level 3, a distance of 0.6 m and an angle of 20°; and for level 4, a distance of 0.9 m and an angle of 40°. The control efficiency showed a significant positive correlation with soluble sugar (SS) content and the activities of superoxide dismutase (SOD), peroxidase (POD) and phenylalanine ammonia-lyase (PAL). Among these, SOD and POD activities were identified as key physiological indicators for evaluating the physiological impact of chemical control on jujube leaf black spot across different severity levels.

By investigating the graded control efficiency of spraying parameters on jujube leaf black spot, this study screened the optimal spraying parameters for graded disease control and identified key physiological indicators for evaluating control efficiency, providing a theoretical basis for the green control of jujube leaf black spot.

## Linked entities

- **Proteins:** peroxidase (peroxidase PPOD1-like)

## Full-text entities

- **Genes:** PAL [NCBI Gene 107413687], POD [NCBI Gene 107420772], Catalase [NCBI Gene 107419839]
- **Diseases:** black (MESH:D007898), leaf damage (MESH:D020263), lesion (MESH:D009059), SS (MESH:C565532), Jujube leaf black spot (MESH:D008796), Jujube leaf black spot disease (MESH:D055008), foliar disease (MESH:D004194), tree diseases (MESH:D021184), fungal diseases (MESH:D009181), plant (MESH:D010939), infection (MESH:D007239)
- **Chemicals:** Fluopyram (MESH:C572868), fluorine (MESH:D005461), water (MESH:D014867), TBA (MESH:C029684), Tebuconazole (MESH:C087114), nitrogen (MESH:D009584), ethylene (MESH:C036216), carbon (MESH:D002244), prothioconazole (MESH:C550005), Sugar (MESH:D000073893), membrane lipid (MESH:D008563), flufenacet (MESH:C094127), Fluoxastrobin (MESH:C550006), ROS (MESH:D017382), lignin (MESH:D008031), sterol (MESH:D013261), lipid (MESH:D008055), ergosterol (MESH:D004875), guaiacol (MESH:D006139), NBT (MESH:D009580), N-phenylphthalamic acid (MESH:C073724), MDA (MESH:D008315), phenylalanine (MESH:D010649), benzimidazoles (MESH:D001562), SS (-), hydrogen peroxide (MESH:D006861), anthrone (MESH:C004522)
- **Species:** Malus domestica (apple, species) [taxon 3750], Ananas comosus (pineapple, species) [taxon 4615], Alternaria alternata (species) [taxon 5599], Ziziphus jujuba (Chinese jujube, species) [taxon 326968]
- **Mutations:** A 20 L

## Full text

_Full body text omitted from this summary view._ Fetch the complete paper as Markdown: https://tomesphere.com/paper/PMC12934512/full.md

## Figures

12 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12934512/full.md

## References

49 references — full list in the complete paper: https://tomesphere.com/paper/PMC12934512/full.md

---
Source: https://tomesphere.com/paper/PMC12934512