# Polymorphisms in the 3′UTR of the TGF-β1 gene associated with litter size in Ujimqin and Sonid sheep

## TL;DR

This study found genetic variations in the TGF-β1 gene linked to litter size in two sheep breeds, offering potential markers for improving reproductive traits.

## Contribution

The study identifies novel TGF-β1 gene polymorphisms associated with litter size in Ujimqin and Sonid sheep.

## Key findings

- Sonid-LD1 and Ujimqin-LD1 polymorphisms in the TGF-β1 gene are significantly linked to litter size in their respective breeds.
- A single polymorphism (g.50044837 C > T) in the TGF-β1 gene is associated with litter size in Sonid sheep.
- These findings suggest potential genetic markers for enhancing sheep reproductive performance.

## Abstract

Reproductive characteristics like ovulation frequency and litter number significantly impact the sheep industry. Transforming growth factor beta 1 (TGF-β1) functions as a multifunctional regulator of key reproductive processes, plays a pivotal role in mammalian ovarian development. However, research on the role of the TGF-β1 gene in the reproduction of Ujimqin and Sonid sheep breeds remains scarce. Thus, this study identified 11 new variants of the TGF-β1 gene in these breeds by direct sequencing. In these 11 variants, the Sonid-linkage disequilibrium (LD)1 and Ujimqin-LD1 (comprising the g.50044287 G > A and g.50044526 G > C polymorphisms) in the 3′ untranslated region of the TGF-β1 gene were significantly associated with litter size in Sonid and Ujimqin ewes (p < 0.01, p < 0.05, respectively), and the single g.50044837 C > T polymorphism in the 3′UTR was also associated with litter size in Sonid sheep (p < 0.01) through association analyses. These findings may provide potential genetic markers for improving prolificacy in sheep.