# Anatomical evaluation and cone-beam computed tomography of some maxillary nerve block approaches in dog

**Authors:** Kaveh Khazaeel, Anahita Memardezfouli, Hadi Imani Rastabi, Seyed Arman Mohagheghi

PMC · DOI: 10.1016/j.vas.2026.100599 · Veterinary and Animal Science · 2026-02-14

## TL;DR

This study compares different methods for blocking the maxillary nerve in dogs and finds the infraorbital approach to be the most effective for pain management.

## Contribution

The study provides new comparative anatomical and radiological evidence supporting the infraorbital approach for maxillary nerve block in dogs.

## Key findings

- The infraorbital approach showed the greatest nerve staining and diffusion depth.
- CBCT scans accurately reflected injection spread and matched dissection results.
- The infraorbital method is the most consistent and effective for maxillary nerve blockade.

## Abstract

•Infraorbital approach showed greatest maxillary nerve staining and diffusion depth.•CBCT accurately demonstrated the spread of the injection and correlated with the dissection.•Infraorbital method provides most consistent and effective maxillary blockade.•Anatomical dissection confirmed the imaging and injection findings.

Infraorbital approach showed greatest maxillary nerve staining and diffusion depth.

CBCT accurately demonstrated the spread of the injection and correlated with the dissection.

Infraorbital method provides most consistent and effective maxillary blockade.

Anatomical dissection confirmed the imaging and injection findings.

Effective pain management is a cornerstone of modern veterinary surgical care, with regional nerve blocks as a crucial tool for targeted analgesia. The maxillary nerve, a major sensory branch of the trigeminal nerve, innervates numerous facial and oral structures, making its blockade essential in oral and maxillofacial procedures in dogs. Despite several described approaches namely the subzygomatic, infraorbital, and maxillary tuberosity techniques, there remains limited comparative anatomical and radiological data to guide clinical decision-making. This study evaluated these three approaches using anatomical dissection and cone-beam computed tomography (CBCT) in six canine cadavers (n = 6). Each received an injectate (methylene blue, iohexol, and bupivacaine) via the three techniques. Pre- and post-injection CBCT scans were analyzed using NNT Viewer v3 and OnDemand3D software, measuring contrast spread in sagittal and dorsal planes (1- and 10-mm slices) and correlating with dissections. Results demonstrated that the infraorbital approach had significantly higher staining of the maxillary nerve compared to the maxillary tuberosity and subzygomatic approaches (P < 0.05). The maximum amount of diffusion of the contrast medium along the maxillary nerve was for the infraorbital approach. Sagittal (10 mm) and dorsal (1 mm) sections had the highest compliance with the length of dye diffusion measured in the anatomical sections. In the maxillary tuberosity approach, sagittal and dorsal views with a thickness of 10 mm had the highest compliance with the length of dye diffusion measured in the anatomical sections. The infraorbital method provided the most consistent and effective blockade, suggesting its preferential use in clinical practice for optimized analgesia.

## Linked entities

- **Chemicals:** methylene blue (PubChem CID 4139), iohexol (PubChem CID 3730), bupivacaine (PubChem CID 2474)

## Full-text entities

- **Diseases:** postoperative pain (MESH:D010149), root fractures (MESH:D011843), ocular trauma (MESH:D014947), pain (MESH:D010146), analgesia (MESH:D000699), maxillary nerve (MESH:D008441), nerve block (MESH:D006327), cranial trauma (MESH:D020197), maxillary nerve blockade (MESH:D008439), tooth extractions (MESH:D014076)
- **Chemicals:** iohexol (MESH:D007472), Methylene blue (MESH:D008751), water (MESH:D014867), IO (-), bupivacaine (MESH:D002045)
- **Species:** Equus asinus (African ass, species) [taxon 9793], Canis lupus familiaris (dog, subspecies) [taxon 9615], Homo sapiens (human, species) [taxon 9606], Felis catus (cat, species) [taxon 9685], Equus caballus (domestic horse, species) [taxon 9796]

## Full text

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## Figures

7 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12933780/full.md

## References

43 references — full list in the complete paper: https://tomesphere.com/paper/PMC12933780/full.md

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Source: https://tomesphere.com/paper/PMC12933780