# Resveratrol protects against isoflurane-induced testicular injury: a stereological and molecular study in mice

## TL;DR

Resveratrol may protect mouse testicles from damage caused by isoflurane, an anesthetic, by boosting antioxidants and reducing cell death.

## Contribution

This study demonstrates resveratrol's protective effects against isoflurane-induced testicular toxicity in mice through stereological and molecular analysis.

## Key findings

- Resveratrol mitigated isoflurane-induced reductions in seminiferous tubule volume and length.
- Resveratrol modulated apoptotic genes (Bcl2l1, Bax, Caspase-3) and enhanced antioxidant gene expression (GPX1, Sod1, catalase).
- Higher doses of resveratrol showed greater protective effects against isoflurane toxicity in testicular tissues.

## Abstract

•Resveratrol studied for protection against isoflurane-induced testicular damage.•Sixty C57BL/6 mice were divided into control, isoflurane, and resveratrol groups.•Isoflurane reduced testicular volume, tubular structures, and altered cells.•Resveratrol modulated apoptotic markers and boosted antioxidant gene expression.•Resveratrol shows potential in preventing isoflurane-induced testicular toxicity.

Resveratrol studied for protection against isoflurane-induced testicular damage.

Sixty C57BL/6 mice were divided into control, isoflurane, and resveratrol groups.

Isoflurane reduced testicular volume, tubular structures, and altered cells.

Resveratrol modulated apoptotic markers and boosted antioxidant gene expression.

Resveratrol shows potential in preventing isoflurane-induced testicular toxicity.

This study examines the therapeutic effects of resveratrol, known for its antioxidant and anti-inflammatory properties, in mitigating the harmful effects of isoflurane, an inhalation anesthetic, on testicular tissues in mice.

Sixty adult male C57BL/6 mice were grouped into six categories: control (normal saline), isoflurane only (1.5% for 1-hour/day), low-dose Resveratrol (RSV, 50 mg/kg/day), high-dose RSV (100 mg/kg/day), isoflurane combined with low-dose RSV, and isoflurane combined with high-dose RSV. After 35-days, the mice were euthanized, and their testes were harvested for stereological analysis and the assessment of antioxidant and apoptotic markers.

Exposure to isoflurane resulted in decreased seminiferous tubule volume and length, along with increased interstitial tissue volume of the testis. Co-treatment with resveratrol, especially at higher doses, mitigated these changes. Additionally, resveratrol modulated the expression of the apoptotic genes Bcl2l1, Bax, and Caspase-3, and enhanced the expression of antioxidant genes GPX1, Sod1, and catalase in mice that received isoflurane.

The findings suggest that resveratrol effectively counters isoflurane-induced damage in testicular structures and gene expression, enhancing tubular integrity and antioxidant defense. This indicates a potential therapeutic approach to protect against isoflurane-induced testicular toxicity.