# Enhanced Colon Regeneration in Ulcerative Colitis via Mesenchymal Stem Cell Medium and 17‐β Estradiol

## TL;DR

This study explores how mesenchymal stem cell medium, especially when treated with estradiol, can improve colon healing in rats with ulcerative colitis.

## Contribution

The novel use of estradiol-treated mesenchymal stem cell medium to enhance colon regeneration in ulcerative colitis is introduced.

## Key findings

- MSC and E2-treated MSC groups showed reduced inflammation and disease activity in UC rats.
- Histopathological analysis showed improved colon tissue regeneration in MSC-treated groups.
- E2-treated MSC medium was particularly effective in reducing inflammatory markers.

## Abstract

Ulcerative colitis (UC), a form of inflammatory bowel disease (IBD), increases the risk of colorectal cancer. This study investigates the potential of conditioned medium from mesenchymal stem cells (CMm) treated with 17‐beta‐estradiol (E2) to enhance colon regeneration in UC‐affected rats.

Bone marrow‐derived MSCs from male Wistar rats were cultured with 100 nM E2 for 24 h. UC was induced in three groups: colitis (C), mesenchymal stem cells (MSC), and MSCs + estradiol (MSC + E2). Each group received intraperitoneal injections of 200 µL CMm derived from 2 × 106 cells or E2‐treated CMm three times postinduction. The study evaluated disease activity index (DAI), myeloperoxidase (MPO) levels, nitric oxide (NO), and pro‐inflammatory cytokines (TNF‐α, IL‐1β, IL‐6) in colon tissues, alongside histopathological assessments.

Results showed significant improvements in DAI and reductions in MPO, NO, and cytokine levels in MSC and MSC + E2 groups compared to the C group. Histopathological analysis revealed enhanced colon tissue regeneration in these groups.

These findings suggest that CMm, particularly with E2, may facilitate colonic healing and mitigate inflammation in UC.

A notable decrease in disease symptoms, oxidant levels, and mRNA expression of inflammatory factors was observed in the groups treated with the culture medium of MSCs, especially in those receiving E2.Histopathological analysis revealed a significant enhancement in colonic tissue regeneration in both groups administered culture medium after colitis induction.

A notable decrease in disease symptoms, oxidant levels, and mRNA expression of inflammatory factors was observed in the groups treated with the culture medium of MSCs, especially in those receiving E2.

Histopathological analysis revealed a significant enhancement in colonic tissue regeneration in both groups administered culture medium after colitis induction.