# Dynamic transcriptome unveils transcriptional network associated with chlorophyll degradation in tobacco leaves under high humidity and temperature

## TL;DR

The study explores how high humidity and temperature affect chlorophyll breakdown in tobacco leaves, revealing key genes and networks involved in the process.

## Contribution

The study identifies specific genes and transcriptional networks associated with chlorophyll degradation under high humidity and temperature in tobacco.

## Key findings

- Chlorophyll degradation rate was twofold higher at 93% RH compared to 79% RH.
- Genes like PAO2, CLH6, SGR6, and CLH5 showed stage-specific expression under different curing conditions.
- Transcription factors such as ERF, NAC, MYB, and WRKY were linked to chlorophyll catabolic gene expression.

## Abstract

Chlorophyll degradation is a key determinant of postharvest quality in flue-cured tobacco. Excessive nitrogen application increases chlorophyll accumulation, leading to green discoloration or mottling and reducing market value after curing.

This study investigated the effects of environmental humidity on chlorophyll degradation through gene ontology (GO) enrichment analysis, differentially expressed genes (DEGs) analysis, and correlation analysis.

At 93% relative humidity (RH), the rate of chlorophyll degradation was approximately twofold higher than that observed at 79% RH, particularly in nitrogen-imbalanced tobacco leaves. Transcriptome analysis of 63 samples revealed stage-specific expression patterns of chlorophyll catabolic genes (CCGs) under different curing conditions. PAO2 showed pronounced expression at 12 h, whereas CLH6 exhibited high expression at 18 h under high humidity. Under high-temperature stress during curing, SGR6 and CLH5 were strongly induced. Correlation-based transcriptional network analysis indicated that multiple transcription factor families, including ERF, NAC, MYB, and WRKY, were closely associated with CCG expression in response to humidity and temperature changes. Together, these findings suggest a complex transcriptional landscape linking environmental conditions with chlorophyll degradation during tobacco curing.

The identified genes and transcriptional associations provide potential molecular candidates for future functional validation and the improvement of curing compatibility in tobacco.