# Silver nanoparticles-based green fluorescent probe for determination of Bosentan in pharmaceutical formulation and spiked plasma samples

## TL;DR

A new eco-friendly method using silver nanoparticles was developed to detect Bosentan in pharmaceutical and plasma samples.

## Contribution

A sensitive and green spectrofluorometric method using AgNPs for Bosentan detection was developed and validated.

## Key findings

- The method uses collisional quenching of AgNPs fluorescence to detect Bosentan in the range of 0.02 to 0.12 µg/mL.
- The method achieved high greenness scores of 80 (AES) and 0.68 (AGREE).

## Abstract

In this work, a new, sensitive, precise, accurate, and eco-friendly spectrofluorometric method for the detection of Bosentan in human plasma and pharmaceutical dosage form was developed, using silver nanoparticles (AgNPs) as an efficient fluorescence sensing probe. The general concept used in this approach is based on collisional quenching of AgNPs fluorescence intensity. Over the concentration range of 0.02 to 0.12 µg/mL, the quenching impact of BOS on AgNPs increased as its concentration increased. The suggested method’s greenness was assessed using two metric systems: the Analytical Eco-scale (AES) with a score of 80 and the AGREE greenness assessment tool with a score of 0.68.

The online version contains supplementary material available at 10.1186/s13065-026-01737-w.