# Spatial Profiling of Germinal Centers in Mouse Secondary Lymphoid Organs Using MACSima Imaging Cyclic Staining Technology

## TL;DR

This paper introduces a new imaging method to study the structure of germinal centers in mouse lymphoid organs.

## Contribution

The novel contribution is a 50-plex imaging protocol with a new analysis method for detecting stromal cell morphology changes.

## Key findings

- A 50-plex imaging protocol was developed for germinal center microarchitecture.
- A new analysis method detects morphological changes in irregularly shaped stromal cells.
- The workflow includes tissue processing, cyclic imaging, and image preprocessing.

## Abstract

We developed a 50‐plex imaging protocol using the MACSima platform to characterize the microarchitecture of germinal centers in secondary lymphoid tissues. This workflow combines tissue processing, automated cyclic imaging, image preprocessing, and a new analysis method that detects morphological changes in irregularly shaped stromal cells.