# Conformational Trajectory of the Molecular Chameleon Grazoprevir From Formulation to Target‐Bound

## TL;DR

This study explores how the macrocycle grazoprevir changes shape from its solid form to when it binds to a target, revealing insights into its flexibility and drug-like properties.

## Contribution

The paper provides a detailed conformational trajectory of grazoprevir using MicroED and NMR, revealing a consistent core structure and adaptive side chain behavior.

## Key findings

- A low-energy core conformation of grazoprevir remains consistent across different environments.
- The vinyl cyclopropyl sulfonamide side chain reorients during the conformational journey.
- In apolar environments, grazoprevir adopts compact conformations with reduced polar surface area, likely enhancing cell permeability.

## Abstract

Macrocycles represent a promising class of beyond‐rule‐of‐5 (bRo5) therapeutics, capable of targeting proteins traditionally considered undruggable by conventional small molecules. Macrocycles exhibit intrinsic flexibility and often display a “chameleon‐like” ability to adapt to their environment, thereby enhancing their oral bioavailability. Describing their structures and conformational changes is essential for advancing their development in the bRo5 space. In this study, we present the novel solid‐ and solution‐state structures of the macrocycle grazoprevir, determined using microcrystal electron diffraction (MicroED) and nuclear magnetic resonance (NMR) spectroscopy. A low‐energy core conformation was consistently identified throughout the conformational journey, from solid formulation to solvated states and upon complexation with the biological target, while the vinyl cyclopropyl sulfonamide side chain reoriented. The presence of a common core conformation (RMSD up to 0.273 Å) suggests that grazoprevir adopts a partial pre‐organized state, optimizing its suitability for target binding. In apolar environment, mimicking the cell membrane, intramolecular hydrogen bonding promoted the formation of compact conformations with reduced radii of gyration and solvent accessible 3D polar surface area, likely facilitating cell permeability. This work presents a comprehensive conformational trajectory of a macrocyclic chameleon and provides potential insights into understanding and characterizing cell permeability and chameleonicity of similar bRo5 therapeutics.