# Experimental study of microbial enhanced oil recovery in fractured porous media using the halophilic bacterium Haloferax mediterranei

**Authors:** Behnam Zarei Eslam, Rohallah Hashemi, Ali Reza Khaz’ali, Mohammadhadi Jazini, Mohammad Amin Behnam Motlagh

PMC · DOI: 10.1038/s41598-026-38949-y · Scientific Reports · 2026-02-05

## TL;DR

This study explores using the halophilic bacterium Haloferax mediterranei to improve oil recovery in fractured reservoirs, finding that optimal biomass concentration enhances recovery while excessive amounts reduce it.

## Contribution

The study introduces optimized biomass concentration for microbial enhanced oil recovery in fractured porous media using Haloferax mediterranei.

## Key findings

- Optimal biomass concentration (5.07 g/L) increased oil recovery by 23% through selective plugging.
- Higher biomass concentrations reduced oil recovery due to excessive polymer production blocking pathways.
- Permeability reductions of 53.5%, 62.5%, and 73.5% were observed for different biomass concentrations.

## Abstract

Fractured reservoirs, comprising over 20% of the world’s oil reserves, present significant challenges to enhanced oil recovery (EOR). This study investigates the potential of the halophilic bacterium Haloferax mediterranei in microbial EOR (MEOR) for improving oil recovery in fractured porous media. Experiments using a heterogeneous glass micromodel were conducted at biomass concentrations of 5.07, 6.74, and 10.14 g/L. Results showed that the optimal biomass concentration (5.07 g/L) increased oil recovery by 23% through selective plugging. Higher biomass concentrations caused excessive polymer production, blocking communication pathways between the matrix and fractures, reducing oil recovery to 11.7% and 7.8%. Permeability reductions were evaluated in two core samples, achieving average reductions of 53.5%, 62.5%, and 73.5% for the respective biomass concentrations. Using the optimal biomass concentration (5.07 g/L), oil recovery in two similar core samples increased by 14% and 12.6%. These findings underscore the importance of optimizing biomass concentration to balance permeability reduction and oil recovery efficiency. The study highlights MEOR’s potential as a cost-effective, sustainable strategy for enhancing oil recovery in fractured reservoirs. It also provides valuable insights for improving future EOR operations, contributing to global energy resource sustainability.

## Linked entities

- **Species:** Haloferax mediterranei (taxon 2252)

## Full-text entities

- **Chemicals:** polymer (MESH:D011108), oil (MESH:D009821)
- **Species:** Haloferax mediterranei (species) [taxon 2252]

## Full text

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## Figures

23 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12929581/full.md

## References

15 references — full list in the complete paper: https://tomesphere.com/paper/PMC12929581/full.md

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Source: https://tomesphere.com/paper/PMC12929581