# An In Silico Omics Investigation of the Lactobacillus Genus Complex for Allergenicity Mitigation

## TL;DR

This study uses genome and protein data to find Lactobacillus strains that may help reduce allergic reactions.

## Contribution

The study identifies Latilactobacillus sakei as a potential probiotic for allergenicity mitigation through in silico omics analysis.

## Key findings

- Latilactobacillus sakei was identified as a promising strain for allergenicity mitigation based on genomic and proteomic features.
- The study analyzed 161 Lactobacillus genomes and proteomes for immunosuppressive motifs and allergen epitopes.
- Bioinformatics tools revealed potential candidates for managing allergies through nutritional interventions.

## Abstract

Among the useful methods of immune response modulation, probiotics can be included. A delicate balance in the microbiome is of extreme importance for immune homeostasis, the composition of which is influenced by lifestyle and dietary habits. The aim of this study was to identify Lactobacillus strains with potential allergenicity‐mitigating properties through comprehensive in silico analysis of their genomes and proteomes. Abrogation in such a balance may lead to the development of food allergy. So far, certain components in probiotics, including immunosuppressive motifs or metabolites like 17,18‐epoxy eicosatetraenoic acid (17,18 EpETE), seem to have the potential to reduce prevalence. However, some bacterial proteins may be allergenic or may induce cross‐reactivity.

We performed an in silico study over the 161 reference genomes and proteomes of Lactobacillus. We analyzed the presence of Nucleotide immunosuppressive motifs (NISM)s, calculated the per million frequency of bases, and checked for the presence of the CYP450 enzymes/stable allergen epitopes. This was evaluated using various bioinformatics tools like Uniprot proteomes, NCBI genomes, AlgPred, PeptideCutter, EMBOSS, and MAST.

Our analysis identified Latilactobacillus sakei as a promising candidate, based on its genomic and proteomic features, for mitigating allergenicity.

Latilactobacillus sakei might be a useful candidate for nutritional allergy management and related conditions. However, this potential requires verification through further experimental studies.

This graphical abstract illustrates the most promising Lactobacillus species for reducing allergenicity through an omics approach, integrating genomics and proteomics studies. Also, provides a clear overview of candidate strains that could be effectively utilized in mitigating allergic reactions.