# Splice choices at the threshold of activation: Alternative splicing fine-tunes Notch signaling in muscle stem cells

## TL;DR

The paper shows how alternative splicing regulates Notch signaling in muscle stem cells through a specific RNA-binding protein and exon inclusion.

## Contribution

The study reveals a novel RBFOX2-dependent splicing event in Numb that fine-tunes Notch signaling during muscle stem cell activation.

## Key findings

- Alternative splicing is widespread during muscle stem cell activation.
- RBFOX2-dependent splicing of Numb regulates Notch signaling.
- Exon inclusion in Numb tunes the transition from quiescence to activation.

## Abstract

Alternative splicing events have emerged as a rapid regulatory layer in gene expression. Lin et al. demonstrate that alternative splicing is widespread during muscle stem cell activation. Its functional importance is illustrated through an RNA-binding fox 1-homolog 2 (RBFOX2)-dependent splice choice in the Notch regulator Numb, showing how inclusion of a single exon can tune Notch signaling to regulate the transition from quiescence to activation.

Alternative splicing events have emerged as a rapid regulatory layer in gene expression. Lin et al. demonstrate that alternative splicing is widespread during muscle stem cell activation. Its functional importance is illustrated through an RNA-binding fox 1-homolog 2 (RBFOX2)-dependent splice choice in the Notch regulator Numb, showing how inclusion of a single exon can tune Notch signaling to regulate the transition from quiescence to activation.