# Protocol for establishing a mouse model of bilateral maxillary first molar extraction to study alveolar bone healing

## TL;DR

This paper provides a detailed protocol for creating a mouse model to study alveolar bone healing after tooth extraction.

## Contribution

A reproducible mouse model protocol for studying alveolar bone healing after bilateral maxillary molar extraction.

## Key findings

- Steps for minimally invasive extraction of bilateral maxillary first molars in mice are described.
- Practical strategies are provided to minimize root fracture and soft tissue injury during the procedure.

## Abstract

Alveolar bone healing following tooth extraction is a complex biological process. Here, we present a protocol for establishing a reproducible mouse model of bilateral maxillary first molar extraction to study alveolar bone healing. We describe steps for surgery, postoperative care, and tissue collection for downstream genomic, histological, and micro-computed tomography (CT) analyses. We provide practical strategies to minimize root fracture and soft tissue injury, ensuring procedural consistency and high-quality outcomes.

•Steps for minimally invasive extraction of bilateral maxillary first molars in mice•Instructions for optimizing oral access using rubber bands and cotton balls•Guidance for intact tooth removal using syringe needles and micro-toothed forceps•Procedures for collecting alveolar bone tissue for histological and micro-CT analyses

Steps for minimally invasive extraction of bilateral maxillary first molars in mice

Instructions for optimizing oral access using rubber bands and cotton balls

Guidance for intact tooth removal using syringe needles and micro-toothed forceps

Procedures for collecting alveolar bone tissue for histological and micro-CT analyses

Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Alveolar bone healing following tooth extraction is a complex biological process. Here, we present a protocol for establishing a reproducible mouse model of bilateral maxillary first molar extraction to study alveolar bone healing. We describe steps for surgery, postoperative care, and tissue collection for downstream genomic, histological, and micro-computed tomography (CT) analyses. We provide practical strategies to minimize root fracture and soft tissue injury, ensuring procedural consistency and high-quality outcomes.