# Ultrasonic driven resveratrol induced egg-derived amyloid-like fibrils hydrocolloids for stabilizing aqueous cyanidin-3-O-glucoside

## TL;DR

This study shows how ultrasound and resveratrol can create a stable hydrocolloid to protect and deliver the antioxidant cyanidin-3-O-glucoside more effectively.

## Contribution

A novel ultrasound-driven, resveratrol-induced hydrocolloid system for stabilizing and enhancing the bioavailability of cyanidin-3-O-glucoside.

## Key findings

- Resveratrol under ultrasound promotes fibril cross-linking and forms a 3D hydrocolloid network.
- The hydrocolloid improves the stability and bioaccessibility of cyanidin-3-O-glucoside during storage and digestion.
- The system enhances antioxidant activity and protects cells from oxidative stress.

## Abstract

This study investigated a low-frequency ultrasound-driven, resveratrol-induced hydrocolloid composed of ovalbumin and lysozyme amyloid-like fibrils for the efficient stabilization of aqueous cyanidin-3-O-glucoside. Results demonstrated that resveratrol, under ultrasonic treatment, acted as a molecular bridge, disrupting the long-range order of linear β-sheets and promoting fibril cross-linking via intramolecular hydrogen bonds. This process significantly reduced the average particle size of the fibrils, transforming the system into a three-dimensional hydrocolloid network with improved viscoelasticity. The resulting hydrocolloid with adjusted surface hydrophobicity and surface hydrophobicity effectively stabilized emulsion- hydrocolloids loaded with cyanidin-3-O-glucoside, yielding uniform droplets with enhanced stability against aggregation and oxidation during storage. The confidence interval of the Cole-Cole curve of the emulsion hydrocolloid narrated and linearized with the increase in resveratrol ratio, and the formed single relaxation network microstructure significantly enhanced the environmental stimulation stability of the emulsion hydrocolloid. In vitro digestion studies revealed that prepared emulsion-hydrocolloids significantly enhanced the bioaccessibility of cyanidin-3-O-glucoside. Furthermore, in a Caco-2 cell monolayer model, the digestively derived micelles from the emulsion-hydrocolloid notably improved the absorption rate of cyanidin-3-O-glucoside over 120 min and exhibited strong cytoprotective effects under H2O2-induced oxidative stress by effectively reducing intracellular ROS levels, increasing SOD activity, and decreasing MDA content. All treatments maintained high cell viability (>95%), indicating excellent biocompatibility. These findings suggest that the ultrasound-assisted resveratrol-induced fibril hydrocolloid is a highly promising vehicle for improving the stability, bioavailability, and bioactivity of hydrophilic polyphenols.