# Anthocyanins from fruit and vegetable waste: biosynthesis, extraction, and gut health benefits

**Authors:** Hudda Ayub, Husnat Ahmad, Syeda Hijab Zehra, Khadija Ramzan, Muhammad Adnan Arif, Naima Tariq, Maria Teresa Capucchio, Robert Mugabi, Aanchal Sharma, Gulzar Ahmad Nayik

PMC · DOI: 10.1016/j.fochx.2026.103614 · 2026-02-02

## TL;DR

This review explores how anthocyanins from fruit and vegetable waste can be extracted and used to improve gut health and create sustainable food products.

## Contribution

The paper uniquely focuses on waste-derived anthocyanins and their microbiome-modulating effects.

## Key findings

- Green extraction techniques improve anthocyanin recovery and sustainability.
- Stabilization methods enhance anthocyanin bioavailability and industrial use.
- Anthocyanins support gut health and may prevent chronic diseases.

## Abstract

Anthocyanins (ANCs), naturally abundant in fruit and vegetable waste, represent a promising class of sustainable functional ingredients with notable gut-related health benefits. This review uniquely focuses on waste-derived anthocyanins and their microbiome-modulating effects, including enhancement of intestinal barrier integrity, prebiotic-like activity, and short-chain fatty acid production. Advances in green extraction and purification techniques such as ultrasound-, microwave-, enzyme-assisted, pulsed electric field, and deep eutectic solvent-based methods have improved recovery efficiency while promoting environmental sustainability. Furthermore, stabilization strategies including acylation, protein/polysaccharide complexation, co-pigmentation, and nano/microencapsulation enhance anthocyanin bioavailability and industrial applicability. By integrating technological innovation with gut health insights, this review highlights emerging opportunities to valorize agro-industrial waste and develop next-generation nutraceutical and functional food systems.

•Anthocyanins recovered from fruit and vegetable processing waste.•Traditional and green extractions influence anthocyanin yield, purity.•Purification, quantification, and stabilization ensure anthocyanin integrity.•Applications include food colorants, functional additives, and nutraceuticals.•Anthocyanins promote gut health and protect against chronic diseases.

Anthocyanins recovered from fruit and vegetable processing waste.

Traditional and green extractions influence anthocyanin yield, purity.

Purification, quantification, and stabilization ensure anthocyanin integrity.

Applications include food colorants, functional additives, and nutraceuticals.

Anthocyanins promote gut health and protect against chronic diseases.

## Linked entities

- **Chemicals:** anthocyanins (PubChem CID 145858)

## Full-text entities

- **Genes:** IL6 (interleukin 6) [NCBI Gene 399500] {aka IL-6}, ICAM1 (intercellular adhesion molecule 1) [NCBI Gene 3383] {aka BB2, CD54, P3.58}, NFKB1 (nuclear factor kappa B subunit 1) [NCBI Gene 4790] {aka CVID12, EBP-1, KBF1, NF-kB, NF-kB1, NF-kappa-B1}, OCLN (occludin) [NCBI Gene 397236], Psen1 (presenilin 1) [NCBI Gene 19164] {aka Ad3h, PS-1, PS1, S182}, N4BP3 (NEDD4 binding protein 3) [NCBI Gene 100511119], NOX4 (NADPH oxidase 4) [NCBI Gene 50507] {aka KOX, KOX-1, RENOX}, TNF (tumor necrosis factor) [NCBI Gene 397086] {aka TNFSF2, TNFa}, zonula occludens-1 [NCBI Gene 396567], NFKBIA (NFKB inhibitor alpha) [NCBI Gene 406188] {aka ECI-6/IKBA, IKBA}, TLR4 (toll like receptor 4) [NCBI Gene 399541], Nup62 (nucleoporin 62) [NCBI Gene 18226] {aka D7Ertd649e, Nupc1, p62}
- **Diseases:** neurological disorders (MESH:D009461), purine metabolism disorders (MESH:D011686), stroke (MESH:D020521), autoimmune disorders (MESH:D001327), obesity (MESH:D009765), Alzheimer's disease (MESH:D000544), diabetes (MESH:D003920), cancer (MESH:D009369), vision impairment (MESH:D014786), acute coronary syndromes (MESH:D054058), gout (MESH:D006073), Neurodegenerative illnesses (MESH:D019636), trauma (MESH:D014947), gastrointestinal inflammation (MESH:D007249), hyperglycemia (MESH:D006943), chronic diseases (MESH:D002908), Hyperuricemia (MESH:D033461), coronary artery disease (MESH:D003324), neuronal injury (MESH:D009410), diabetes mellitus type 2 (MESH:D003924), EAE (MESH:D008661), infarction of the heart (MESH:D007238), kidney impairment (MESH:D007674), inflammatory bowel conditions (MESH:D015212), cytotoxicity (MESH:D064420), insulin resistance (MESH:D007333), heart attack (MESH:D009203), cardiovascular disease (MESH:D002318), diabetic retinopathy (MESH:D003930), colitis (MESH:D003092), carcinogenic (MESH:D011230), Co-pigmentation (MESH:D060085)
- **Chemicals:** xanthan gum (MESH:C002563), ethanol (MESH:D000431), blood glucose (MESH:D001786), galactose (MESH:D005690), malvidin-3-O-glucoside (MESH:C000706890), lauric acid (MESH:C030358), ethyl lactate (MESH:C015866), HCl (MESH:D006851), aglycones (MESH:C458179), CMC (MESH:C514968), catechol (MESH:C034221), paeoniflorin (MESH:C015423), disaccharide (MESH:D004187), phenolic acids (MESH:C017616), (-)-epicatechin (MESH:D002392), hyperoside (MESH:C021304), water (MESH:D014867), phenol (MESH:D019800), cyanidin-3-O-rutinoside (MESH:C428983), carotenoids (MESH:D002338), free radicals (MESH:D005609), kaempferol (MESH:C006552), DPPH (MESH:C004931), acetonitrile (MESH:C032159), malvidin 3-galactoside (MESH:C458399), monosaccharides (MESH:D009005), chlorophylls (MESH:D002734), konjac glucomannan (MESH:C022901), methyl salicylate (MESH:C033069), rutin (MESH:D012431), polysaccharide (MESH:D011134), lead acetate (MESH:C008261), lactic acid (MESH:D019344), pectin (MESH:D010368), uric acid (MESH:D014527), choline chloride (MESH:D002794), quercetin (MESH:D011794), acids (MESH:D000143), sugar (MESH:D000073893), chitosan (MESH:D048271), maltodextrin (MESH:C008315), allopurinol (MESH:D000493), O2 (MESH:D010100), maleic acid (MESH:C030272), glucoside (MESH:D005960), sodium alginate (MESH:D000464), chalcones (MESH:D047188), metal (MESH:D008670), flavanone (MESH:C028610), pelargonidin (MESH:C066957), chalcone (MESH:D002599), silica gel (MESH:D058428), methanol (MESH:D000432), Amberlite (MESH:C046797), glucose (MESH:D005947), CGA (MESH:D002726), cyanidin 3-glucoside (MESH:C462279), flavonoid (MESH:D005419), Dowex (MESH:C027972), beta-glucan (MESH:D047071)
- **Species:** Citrus x limon (lemon, species) [taxon 2708], Roseburia (genus) [taxon 841], Sus scrofa (pig, species) [taxon 9823], Desulfovibrio (genus) [taxon 872], Brassica oleracea (wild cabbage, species) [taxon 3712], Lactobacillus (genus) [taxon 1578], Brassica oleracea var. viridis (collards, varietas) [taxon 3713], Hibiscus sabdariffa (red-sorrel, species) [taxon 183260], Mus musculus (house mouse, species) [taxon 10090], Escherichia coli (E. coli, species) [taxon 562], Malus domestica (apple, species) [taxon 3750], Clostridium perfringens (species) [taxon 1502], Lonicera caerulea (blue honeysuckle, species) [taxon 134520], Ribes nigrum (European black currant, species) [taxon 78511], gut metagenome (species) [taxon 749906], Allium cepa (onion, species) [taxon 4679], Oryza sativa (Asian cultivated rice, species) [taxon 4530], Solanum tuberosum (potatoes, species) [taxon 4113], Enterobacteriaceae (enterobacteria, family) [taxon 543], Bifidobacterium (genus) [taxon 1678], Faecalibacterium prausnitzii (species) [taxon 853], Homo sapiens (human, species) [taxon 9606], Rattus norvegicus (brown rat, species) [taxon 10116], Bacteria Latreille et al. 1825 (Bacteria stick insect, genus) [taxon 629395], Daucus carota (carrot, species) [taxon 4039], Akkermansia muciniphila (species) [taxon 239935]
- **Cell lines:** BV-2 — Mus musculus (Mouse), Transformed cell line (CVCL_0182)

## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC12925224/full.md

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Source: https://tomesphere.com/paper/PMC12925224