Label-free quantitative proteomics analysis of sea cucumber (Apostichopus japonicus) from different origins using data-independent acquisition mass spectrometry

TL;DR

This study uses advanced mass spectrometry to identify proteins that explain why sea cucumbers from Liaoning, China, have higher quality collagen.

Contribution

A novel label-free DIA workflow was developed to uncover proteomic differences linked to geographical origin in sea cucumbers.

Findings

64 differentially expressed proteins were identified, including arginase and glutamine synthetase as potential markers.

Arginine biosynthesis was highlighted as a key pathway (P = 0.00034) in Liaoning sea cucumbers.

The study links nitrogen metabolism and stress adaptation to regional quality differences in sea cucumbers.

Abstract

Sea cucumbers (Apostichopus japonicus) from Liaoning, China command premium market value due to superior collagen integrity, yet the proteomic basis of this geographical differentiation remains unclear. Current geographic proteomic studies using data-dependent acquisition (DDA) mass spectrometry face stochastic ion selection bias, poor low-abundance reproducibility and limited coverage. Here, we established a custom label-free data-independent acquisition (DIA) workflow to characterize proteomic profiles of 200 sea cucumbers (80 Liaoning, 120 non-Liaoning). Our approach identified 6278 proteins and discovered 64 differentially expressed proteins (DEPs), including arginase (A0A141R8A8) and glutamine synthetase (A0A076VCY3) as potential authentication markers. Functional annotation highlighted arginine biosynthesis (P = 0.00034) as the pivotal pathway, alongside two-component system and necroptosis, indicating nitrogen homeostasis prioritization and stress adaptation in Liaoning specimens. This study provides a novel DIA-based framework for discovering the proteomic basis of geographical differentiation, linking nitrogen metabolism to regional quality differences in marine foods and identifying potential molecular targets for future authentication efforts.

•A label-free DDA-library based DIA workflow was customized for the analysis of sea cucumber proteome.•The proteomic differences of 200 sea cucumbers from different origins were analyzed and 6278 proteins were identified.•Arginine biosynthesis pathway (P = 0.00034) drives Liaoning sea cucumber adaptation.•64 DEPs linked to nitrogen homeostasis, stress response, and environmental sensing.•Establishes a proteomic framework for marine food origin authentication.

A label-free DDA-library based DIA workflow was customized for the analysis of sea cucumber proteome.

The proteomic differences of 200 sea cucumbers from different origins were analyzed and 6278 proteins were identified.

Arginine biosynthesis pathway (P = 0.00034) drives Liaoning sea cucumber adaptation.

64 DEPs linked to nitrogen homeostasis, stress response, and environmental sensing.

Establishes a proteomic framework for marine food origin authentication.

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