# Distinct metabolic signatures of ploidy and cold storage in blueberry fruit revealed by integrative metabolomics and machine learning

## TL;DR

The study shows how cold storage and ploidy affect blueberry metabolism, with sugars and flavonoids being key factors.

## Contribution

The novel integration of metabolomics and machine learning reveals distinct metabolic signatures influenced by ploidy and cold storage.

## Key findings

- Cold storage increases fructose while decreasing glucose, with total sugar remaining stable.
- Flavonoid biosynthesis is the most affected pathway by cold storage.
- Terpenoids are strong predictors of sugar-related quality traits in blueberries.

## Abstract

•Cold storage and ploidy shape the blueberry metabolome independently.•Sugar shifts during storage: more fructose, less glucose, stable total sugar.•Terpenoids are strong predictors of sugar-related quality traits.•Flavonoid biosynthesis is the top pathway affected by cold storage.

Cold storage and ploidy shape the blueberry metabolome independently.

Sugar shifts during storage: more fructose, less glucose, stable total sugar.

Terpenoids are strong predictors of sugar-related quality traits.

Flavonoid biosynthesis is the top pathway affected by cold storage.