# Possible regulation of Arabidopsis MYB93 by both SCARECROW and MPK3

## TL;DR

This study explores how the Arabidopsis MYB93 gene is regulated by two proteins, SCARECROW and MPK3, through experiments and genetic analysis.

## Contribution

The paper provides experimental evidence that MYB93 is regulated by SCARECROW and interacts with MPK3, linking these factors to plant development.

## Key findings

- MYB93 transcript levels are elevated in the scr-3 mutant and reduced with SCR-GFP expression.
- The N-terminal domain of MYB93 interacts with MPK3 in yeast.
- mpk3-1 mutants show a lateral root phenotype similar to myb93-1 mutants.

## Abstract

The promoter of the
Arabidopsis

MYB93

(

MYB93

) transcription factor was previously identified in a large-scale screen using the
SCARECROW
(
SCR
) transcription factor. Independent high-throughput studies also identified
MYB93
as a protein-interaction partner of the MAP
kinase 3
(
MPK3
). Here, we validate and extend those observations using RT-PCR, yeast two-hybrid assays and phenotypic analysis.

MYB93

transcript levels were elevated in the
scr-3
mutant and reduced upon expression of SCR-GFP, indicating regulation by SCR. In yeast, the N-terminal domain of
MYB93
, but not the C-terminal region, interacted with
MPK3
. We also observed that
mpk3-1
mutants exhibited a lateral root phenotype similar to
myb93-1
. Together, these findings support a model in which both

MYB93

gene expression and
MYB93
protein function are modulated by
SCR
and
MPK3
, respectively.