# Correlation Between Neuroimaging and Neuropathologic Findings in Pediatric ECMO Patients

## TL;DR

This study finds that neuroimaging scores correlate with brain damage in children on ECMO, but some injuries are only visible with detailed lab tests.

## Contribution

The study introduces a correlation framework between neuroimaging and neuropathology in pediatric ECMO patients.

## Key findings

- Neuroimaging severity scores strongly correlate with histopathologic severity in frontal, temporal, and deep grey regions.
- Severe pathology consistently corresponds to high NIS scores, while mild pathology aligns with low scores.
- Immunohistochemistry reveals subtle injuries in regions not visible on standard imaging.

## Abstract

Neurologic complications are a leading cause of morbidity and mortality in children supported with extracorporeal membrane oxygenation (ECMO). Antemortem neuroimaging is limited, while postmortem neuropathology provides definitive but rarely available assessment. Correlating imaging with pathology may improve diagnostic accuracy and monitoring strategies.

We performed a retrospective, single-center cohort study of neonates and children (0–18 years) supported with ECMO (2009–2024) who underwent cerebral autopsy. Standardized neuropathologic sampling across six brain regions classified injuries as mild, moderate, or severe. Antemortem imaging (CT or head ultrasound [HUS]) within seven days of death was reviewed by a blinded neuroradiologist and scored using a Neurologic Injury Severity (NIS) framework. Imaging-pathology correlation was assessed in subjects with both datasets.

Fifty-four patients underwent neuropathologic evaluation; 26 had qualifying neuroimaging. Neuropathology revealed frequent ischemic and hemorrhagic injuries, most commonly in the frontal lobes, deep grey matter, and temporal lobes. Severe injury was more frequent in venoarterial ECMO and in infants/toddlers. NIS scores strongly correlated with global and regional histopathologic severity, particularly in frontal, temporal, and deep grey regions. Discordance was observed in parieto-occipital, pontine, and cerebellar regions, where immunohistochemistry (GFAP, CD68) detected subtle injuries not visible on H&E. Severe pathology consistently corresponded to NIS ≥10, while mild pathology aligned with low scores and limited involvement.

Structured imaging severity scoring, especially with CT, correlates strongly with neuropathology in pediatric ECMO decedents. Findings support NIS scoring as a surrogate for underlying pathology, while underscoring the need for refined histologic methods and adjunctive neuromonitoring to optimize neurologic surveillance.