# Long-term regulatory effects of high-fiber low-fat diet on gut microbiota diversity and inflammatory marker expression in DSS-induced recurrent colitis in mice

## TL;DR

A high-fiber low-fat diet helps manage colitis in mice by improving gut bacteria diversity and reducing inflammation over time.

## Contribution

The study reveals the long-term benefits of a high-fiber low-fat diet in modulating gut microbiota and inflammation during recurrent colitis.

## Key findings

- HFLF diet improved clinical symptoms and gut microbiota diversity in mice with recurrent colitis.
- The diet increased beneficial bacteria and short-chain fatty acid production.
- It modulated inflammation and barrier-related markers during recurrence-remission cycles.

## Abstract

To evaluate the long-term regulatory effects of high-fiber low-fat (HFLF) diet on gut microbiota diversity and inflammation/barrier-related markers in DSS-induced recurrent colitis mice.

A recurrent DSS model was established through multiple cycles of DSS administration. Mice were randomly assigned to HFLF diet vs. control/standard chow ± positive control groups. Multi-timepoint sampling was conducted throughout the study. 16S rRNA sequencing, SCFA quantification, and qPCR/ELISA/immunohistochemistry/Western blot analyses were performed.

HFLF diet significantly improved clinical phenotype, increased gut microbiota diversity, promoted beneficial bacterial genera, enhanced SCFA production, and modulated inflammation/barrier indicators throughout the recurrence-remission cycles.

HFLF diet provides sustained protection during recurrence-remission processes through microbiota-SCFA-inflammation axis modulation, offering potential translational applications for IBD management.