# An Automated Organotypic SCN Culture System Revealing Novel Insights into VIP Regulation of Circadian Rhythm

## TL;DR

A new automated system for studying brain tissue reveals how a protein called VIP helps control circadian rhythms.

## Contribution

The development of the BaSIC system and the discovery of VIP's role in rapidly resetting circadian rhythms via PER2 protein.

## Key findings

- VIP pulsing rapidly resets circadian rhythms by synchronizing phase and amplitude.
- VIP promotes a swift reduction of PER2 protein, as shown through experiments and modeling.

## Abstract

In mammals, the suprachiasmatic nucleus (SCN) functions as the primary driver of circadian rhythm. Although individual SCN neurons exhibit cell‐autonomous oscillations, intercellular communication—mediated by neural peptides such as vasoactive intestinal polypeptide (VIP)—synchronizes their rhythms. However, the precise molecular mechanism by which VIP facilitates SCN synchronization remains elusive. Here, we introduce an automated ex vivo culture system, termed ‘brain‐slice‐in‐a‐chamber’ (BaSIC), tailored for SCN slices, as well as dissociated cells culture. This apparatus automates medium exchange and ensures optimal control over temperature and humidity, ensuring a stable internal environment conducive to tissue culture. Furthermore, BaSIC enables real‐time observation of tissue responses to diverse but programmed stimuli. Using BaSIC, we demonstrate that VIP pulsing rapidly resets the circadian rhythm by synchronizing both phase and amplitude through a swift reduction of Period 2 (PER2) protein. Mathematical modeling, coupled with experimental validation, further suggests that VIP promotes the rapid reduction of PER2. Our findings, facilitated by BaSIC, provide new insights into SCN neuron synchronization, paving the way for advanced studies in chronobiology, with potential therapeutic applications for circadian disorders.

An Automated ex vivo culture system BaSIC, tailored for SCN slices and cell culture, which automates medium exchange and ensures a stable internal environment is developed. BaSIC enables real‐time observation of tissue/cell responses to diverse but programmed stimuli. Using BaSIC, we demonstrate that VIP pulsing rapidly resets the circadian rhythm through a swift reduction of Period 2 (PER2) protein.