# Electron‐Mediator‐Free Microfluidic Photocatalytic Coenzyme Regeneration with 100% Conversion Efficiency within 126 S

## TL;DR

A new microfluidic system regenerates coenzyme NAD(P)H efficiently without electron mediators, achieving full conversion in just over two minutes.

## Contribution

An electron-mediator-free microfluidic platform using BiOBr nanosheets for rapid and stable coenzyme regeneration.

## Key findings

- 100% NAD+ conversion to NADH within 126 seconds with 72.30% selectivity for 1,4-NADH.
- The system maintains >87% efficiency for 32 hours of continuous operation.
- The method offers a sustainable solution for biocatalysis and renewable energy applications.

## Abstract

Microfluidic systems enhance photocatalytic efficiency through superior mass/energy transfer and precise parameter control. Here, an electron‐mediator‐free microfluidic platform is designed for photocatalytic coenzyme NAD(P)H regeneration by integrating ultrathin BiOBr nanosheets. This architecture enables direct electron–proton coupling, achieving 100% NAD+ conversion within 126 s with exceptional selectivity (72.30%) for bioactive 1,4‐NADH. Continuous operation over 32 h shows no activity decay, demonstrating unparalleled stability. This study establishes a benchmark for designing integrated photocatalytic systems and highlights their broad potential for applications in biocatalysis, synthetic biology, and renewable energy.

Discover a groundbreaking mediator‐free photocatalytic method for coenzyme NAD(P)H regeneration using BiOBr nanosheets in a microfluidic chip. This study achieves 100% NAD+ to NADH conversion in 126 s, maintaining >87% efficiency over 32 h. Explore how photocatalysis and microfluidics combine to offer a sustainable, scalable solution for green chemistry.