# A guide to selecting high-performing antibodies for Optineurin (UniProt ID: Q96CV9) for use in western blot, immunoprecipitation, and immunofluorescence

## TL;DR

This paper provides a guide to selecting reliable antibodies for studying the protein Optineurin, which is linked to neurodegenerative diseases.

## Contribution

The study evaluates eight commercial antibodies for Optineurin using standardized protocols and shares results openly to improve antibody reproducibility.

## Key findings

- Eight commercial antibodies for Optineurin were tested using standardized protocols.
- Results are shared openly to help researchers choose high-performing antibodies.
- The study is part of a collaborative initiative to improve antibody reproducibility.

## Abstract

Optineurin (OPTN) is a multifunctional cytoplasmic adaptor protein implicated in maintaining neuronal homeostasis through its roles in selective autophagy, vesicle trafficking, and regulation of inflammatory signaling. Mutations in the
OPTN gene are causally linked to several neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) and primary open-angle glaucoma. Here we have eight optineurin commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While the use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.