# Palmitic acid supplementation enhances milk fat synthesis and energy balance without altering intake or yield in lactating goats

## TL;DR

Adding palmitic acid to goat diets boosts milk fat and energy efficiency without affecting intake or milk yield.

## Contribution

This study provides species-specific evidence on palmitic acid's effects in dairy goats under semi-arid conditions.

## Key findings

- Milk fat concentration and yield increased significantly with palmitic acid supplementation.
- Energy-corrected milk yield rose by ~40% in PA-fed goats.
- Milk FA profile shifted toward higher C16:0 and C16:1 proportions.

## Abstract

Palmitic acid (PA) (C16: 0) is a rumen-inert long-chain fatty acid (FA) widely used in dairy cattle to increase dietary energy density and milk fat synthesis; however, its effects in dairy goats remain poorly characterized. This study evaluated whether supplementing 3% or 6% PA in the diet of mid-lactation goats could improve milk yield, composition, FA profile, and whole-animal energy balance under semi-arid Mexican production conditions.

Twenty-one multiparous crossbred goats (45.8 ± 1.2 kg; 21 ± 3 days in milk) were randomly assigned to three treatments for 6 weeks after a 2-week adaptation: (1) Control diet (without PA), (2) diet + 3% PA, and (3) diet + 6% PA on a dry-matter (DM) basis. Diets were isoenergetic and isoproteic before PA addition. Individual DM intake (DMI), milk yield, and composition were measured daily; milk FA profiles and energy balance were determined on days 0, 21, and 42. Data were analyzed using a mixed-model with repeated measures, and means were compared using the Tukey test (p ≤ 0.05).

PA inclusion did not affect DMI, body weight, or milk yield. However, milk fat concentration and yield increased significantly (p < 0.01) in both PA treatments, with the highest fat concentration observed at 6% PA. The milk FA profile shifted toward greater C16: 0 and C16: 1 proportion (p < 0.0001) and decreased short-chain (<C16) and long-chain (>C16) FA fractions. Energy-corrected milk yield rose by ~40% in PA-fed goats, and energy balance improved markedly from week 3 onward, particularly in the 3% group (p < 0.01), indicating superior dietary energy utilization without intake suppression.

Moderate PA supplementation (~3% DM) effectively enhances milk fat synthesis and energy efficiency in goats while maintaining stable intake and yield. Increasing PA beyond 3% confers minimal additional benefit and may overly saturate milk fat. These findings provide species-specific evidence that rumen-inert fat inclusion can be an efficient strategy to support metabolic status and product quality in mid-lactation goats under variable forage systems.